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Methods Mol Biol ; 2800: 67-74, 2024.
Article in English | MEDLINE | ID: mdl-38709478

ABSTRACT

The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial distribution of numerous pathways of interest simultaneously. Additional data can also be gained by placing the identified proteins into the context of adjacent structures, stroma, and interacting partners. Here, we outline a protocol for using the recently described IBEX method on tissues. This is an open and simple cyclic immunohistochemistry approach suited to this application. We describe a simplified protocol and provide guidance on the method, using a 12-marker panel on human retina to demonstrate the approach.


Subject(s)
Immunohistochemistry , Retina , Signal Transduction , Humans , Immunohistochemistry/methods , Retina/metabolism , Retina/cytology , Biomarkers , Molecular Imaging/methods
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