1.
Proc Natl Acad Sci U S A
; 98(25): 14238-43, 2001 Dec 04.
Article
in English
| MEDLINE
| ID: mdl-11724932
ABSTRACT
Rolling circle amplification has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate that rolling circle amplification in situ can detect gene copy number and single base mutations in fixed cells with efficiencies up to 90%. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.