Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Publication year range
1.
Adv Sci (Weinh) ; 11(12): e2304519, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38227373

ABSTRACT

The regulation of gene expression by light enables the versatile, spatiotemporal manipulation of biological function in bacterial and mammalian cells. Optoribogenetics extends this principle by molecular RNA devices acting on the RNA level whose functions are controlled by the photoinduced interaction of a light-oxygen-voltage photoreceptor with cognate RNA aptamers. Here light-responsive ribozymes, denoted optozymes, which undergo light-dependent self-cleavage and thereby control gene expression are described. This approach transcends existing aptamer-ribozyme chimera strategies that predominantly rely on aptamers binding to small molecules. The optozyme method thus stands to enable the graded, non-invasive, and spatiotemporally resolved control of gene expression. Optozymes are found efficient in bacteria and mammalian cells and usher in hitherto inaccessible optoribogenetic modalities with broad applicability in synthetic and systems biology.


Subject(s)
RNA, Catalytic , RNA , Animals , Nucleotide Motifs , RNA/genetics , RNA, Catalytic/chemistry , RNA, Catalytic/genetics , RNA, Catalytic/metabolism , Bacteria/metabolism , Gene Expression , Mammals/metabolism
2.
Cell Chem Biol ; 27(3): 257-258, 2020 03 19.
Article in Albanian, English | MEDLINE | ID: mdl-32200913

ABSTRACT

In a recent issue of Cell Chemical Biology, Gray et al. (2020) report an aptamer-based method to reversibly label and isolate EGF receptor-expressing cells from heterogeneous mixtures by cell sorting approaches. Subsequent treatment using complementary oligonucleotides restores full functionality of EGF receptors, highlighting the superiority of this method to antibody-based sorting.


Subject(s)
Oligonucleotides , Cell Movement , Ligands , Protein Transport
SELECTION OF CITATIONS
SEARCH DETAIL
...