ABSTRACT
Perilymph has a total protein component that is quantitatively distinct from serum and cerebrospinal fluid (CSF). The goal of this research was to determine if perilymph contains any qualitatively unique protein constituents that will distinguish it from serum or CSF. To test this hypothesis, matched sets of perilymph, serum, and CSF were obtained from 18 guinea pigs and seven human subjects. The purity of each sample was assured by measurement of the protein concentration of each sample and comparison of this parameter to known normal values for perilymph, serum, and CSF. Each sample was then subjected to two-dimensional gel electrophoresis, separating proteins by isoelectric point in the horizontal dimension and by relative molecular weight in the vertical dimension. All gels were processed under precisely identical physical conditions by use of a diamine silver stain. A small number of perilymph proteins not found in plasma were identified in both the guinea pig and the human specimens. The finding of unique perilymph proteins may permit the development of a sensitive marker that will aid in the diagnosis of perilymph fistula.
Subject(s)
Perilymph/chemistry , Proteins/analysis , Animals , Blood Proteins/analysis , Cerebrospinal Fluid Proteins/analysis , Electrophoresis, Gel, Two-Dimensional , Guinea Pigs , Humans , Isoelectric Focusing , Molecular Weight , Neuroma, Acoustic/blood , Neuroma, Acoustic/cerebrospinal fluid , Neuroma, Acoustic/chemistry , Rosaniline Dyes , Spectrophotometry , Staining and LabelingABSTRACT
Intracranial complications of paranasal sinusitis constitute true surgical and medical emergencies. The charts of all patients (n = 649) admitted for acute or chronic sinusitis to the University of Minnesota Hospital and to the University of Michigan Medical Center during a 13-year period (1975 to 1988) were retrospectively reviewed to determine the incidence of complications. The clinical presentation, bacteriology, involved sinuses, influencing host factors, white blood cell count on presentation, length of hospitalization, and postinterventional complications are presented. Twenty-four patients with intracranial complications from paranasal sinusitis are studied for an incidence of 3.7%. Aggressive medical and semi-emergent surgical intervention are required to prevent excessive morbidity and/or mortality. Intracranial complications included subdural empyema, frontal lobe abscesses, intrahemispheric abscesses, cavernous and superior sagittal sinus thrombosis, and osteomyelitis.
Subject(s)
Brain Diseases/etiology , Osteomyelitis/etiology , Sinusitis/complications , Skull , Adolescent , Adult , Aged , Brain Abscess/diagnosis , Brain Abscess/etiology , Brain Diseases/diagnosis , Female , Humans , Length of Stay , Male , Meningitis/diagnosis , Meningitis/etiology , Middle Aged , Osteomyelitis/diagnosis , Retrospective Studies , Sinus Thrombosis, Intracranial/diagnosis , Sinus Thrombosis, Intracranial/etiology , Sinusitis/microbiologyABSTRACT
An example of a rare peripheral ameloblastic carcinoma of the maxillary tuberosity is presented. A discussion of its possible relationship to the dental lamina and malignant ameloblastoma follows.
Subject(s)
Ameloblastoma/pathology , Odontogenic Tumors/pathology , Aged , Ameloblastoma/classification , Ameloblastoma/embryology , Female , Humans , Odontogenic Tumors/classification , Odontogenic Tumors/embryologyABSTRACT
Arthrogryposis multiplex congenita (AMC) is an uncommon congenital disorder characterized by multiple fixed joint deformities and non-progressive neuromuscular dysfunction. A small fraction of these infants will present with otolaryngologic problems resulting from cranial nerve weakness, muscle dysplasia, or structural dysharmony of the head and neck. The charts of 50 patients with AMC were reviewed to determine the incidence of these findings. A summary of the literature is presented discussing the etiology, pathophysiology, diagnosis and management of this interesting clinical problem.
