ABSTRACT
The research was aimed at studying molecular genetics basis of human longevity. The genotyping of polymorphisms in genes of interleukin 6 (IL-6, 7p21, -572G>C, rs1800796), 10 (IL-10, 1q31-q32, -627C>A, rs1800792), 12, (IL-12B, 5q31.1-q33.1, -1159C>A) and tumor necrosis factor-alpha (TNF-alpha, 6p21.3, -308G>A, rs1800629) from ethnically homogeneous population (Tatars from Bashkortostan Republic) has been carried out. Distributions of allele and genotypes frequencies in different age groups including old men and long-livers have been characterized. Associations have been revealed between age and -627C>A polymorphism of IL-10 gene in men, -572G>C polymorphism of IL-6 gene and -308G>A polymorphism of TNF-alpha gene in women. As a whole the data obtained by us confirm the assumption that polymorphism of cytokine genes can influence on human lifespan.
Subject(s)
Cytokines/genetics , Longevity/genetics , Alleles , Bashkiria , Female , Genotype , Humans , Male , Polymorphism, Genetic , Sex FactorsABSTRACT
On sample of 1534 ethnic Tatars catalase (CAT, -262C/T), glutathione peroxidase 1 (GPX1, L198P) and methionine sulfoxide reductase A (MSRA, -402C/T) gene polymorphisms in connection with lifespan were studied. On each separate polymorphic marker nonlinear character of alleles and genotypes frequencies changes with age was revealed. Combinations of genotypes CAT*C/*T-GPX1 *L/*L were significant for elderly and senile age.
Subject(s)
Antioxidants/metabolism , Catalase/genetics , Glutathione Peroxidase/genetics , Longevity/genetics , Oxidoreductases/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Asian People , Child , Child, Preschool , Female , Humans , Infant , Male , Methionine Sulfoxide Reductases , Middle Aged , Young Adult , Glutathione Peroxidase GPX1ABSTRACT
Comparison in genotype and allele frequencies of people groups of younger (from 1 till 20 years), middle (21-55 years), elderly (56-74 years), senile (75-89 years) age and long-livers (90-109 years) have been performed (only 1116 person) with the purpose of analysis of molecular-genetic bases of ageing and longevity of the person. Allele variants of PON1 gene have been identified by polymerase chain reaction in a combination with restriction analysis. In the general sample of Tatars genotypes PON1*Q/*Q, PON1*Q/*R and PON1*R/*R are revealed with frequencies of 46.15, 44.35 and 9.5%, alleles PON1*Q and PON1*R are found with frequencies of 68.32 and 31.68% accordingly. Statistically significant distinctions on frequencies of genotypes and alleles between separate age groups are found. It has appeared, that frequency of PON1*R allele (28.46%) is lowered among old men in comparison with those among persons of younger age (37.42%, P = 0.009). However essentially above in group of long-livers, than in group of old men, frequencies allele PON1*R (P = 0.005) and genotype PON1*R/*R (P = 0.01).