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Article in English | MEDLINE | ID: mdl-23270884

ABSTRACT

Simple, effective and rapid approach for the green synthesis of silver nanoparticles (AgNPs) using leaf extract of Sesbania grandiflora and their in vitro antibacterial activity against selected human pathogens has been demonstrated in the study. Various instrumental techniques were adopted to characterize the synthesized AgNPs viz. UV-Vis, FTIR, XRD, TEM, EDX and AFM. Surface Plasmon spectra for AgNPs are centered at 422 nm with dark brown color. The synthesized AgNPs were found to be spherical in shape with size in the range of 10-25 nm. The presence of water soluble proteins in the leaf extract was identified by FTIR which were found to be responsible for the reduction of silver ions (Ag(+)) to AgNPs. Moreover, the synthesized AgNPs showed potent antibacterial activity against multi-drug resistant (MDR) bacteria such as Salmonella enterica and Staphylococcus aureus.


Subject(s)
Anti-Bacterial Agents/pharmacology , Green Chemistry Technology/methods , Metal Nanoparticles/chemistry , Plant Extracts/chemistry , Plant Leaves/chemistry , Sesbania/chemistry , Silver/chemistry , Bacteria/drug effects , Bacteria/growth & development , Humans , Metal Nanoparticles/ultrastructure , Microbial Sensitivity Tests , Microscopy, Atomic Force , Spectrometry, X-Ray Emission , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared , X-Ray Diffraction
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