Natural mineral spring water (hora) and surrounding soils in southwestern Ethiopia: farmers' feeding practices and their perception about its nutritional roles on animal performance.

Natural mineral water (termed hora in Afan Oromo) and surrounding soils are the most important source of mineral supplement traditionally used for livestock in many parts of Ethiopia. However, limited information exists on feeding practices and the impact of hora on animal performance. Thus, the present study aimed to assess farmers' feeding practices and perceptions about the role of hora and surrounding soils on animal performance. Data were collected from 385 households in four districts (Bedele, Dabo, Gechi and Boracha) through face-to-face interviews using a semi-structured questionnaire with the help of the KoboCollect application. Data were analyzed using SPSS version 26. The majority (72.3 %) of respondents reported hora as an important source of mineral supplement for livestock. About 78.1 % of respondents in Boracha routinely supplement their animals compared with farmers from other districts. Large ruminants were given first priority in supplementation with hora compared to other livestock. Interviewed farmers trekked their animals a distance of 6.5 ± 0.2 km to access hora twice a year during the dry season. The majority of the farmers (68.1 %) believed that hora supplementation improved animal performance, while 35.1 % noted negative impacts such as abortions, birth defects, delayed puberty, decreased conception rates and paralysis due to excessive consumption. This study underscores the perceived importance of hora as a natural source of mineral supplementation for livestock health and productivity. It emphasizes the need for improved management and conservation practices to ensure sustainable utilisation and mitigate negative impacts associated with excessive consumption. Moreover, to validate local farmers' perceptions regarding the importance of hora mineral water in animal nutrition and health, further research is needed to determine mineral composition of hora and livestock responses under controlled feeding trials.

Copper, iron, zinc and tannin concentrations throughout the digestive tract of tropical goats and sheep fed a high-fibre tannin-rich diet.

The dry season in tropical regions urges livestock to feed on nutritionally very poor diets. It has not been explored how tropical sheep-assumed grazers-and tropical goats-intermediate browsers-cope with a high-fibre tannin-rich diet. This study was designed to determine the effects of a high-fibre and tannin-rich diet on the flow of important microminerals iron (Fe), zinc (Zn) and copper (Cu) throughout the digestive tract of tropical sheep and goats. The feeding trial was set up with twelve adult male animals, six sheep with mean body weight (BW) of 30.3 ± 1.6 kg and six goats with mean BW of 26.4 ± 2.2 kg. The feed consisted of 36% leaves of Millettia ferruginea, 61% hay and 3% concentrate and was offered at 3% of BW (all on dry matter (DM) basis). The total faecal collection was carried out for 7 consecutive days. At the end of the experimental period, the animals were slaughtered to collect liver and digesta samples from the gastrointestinal tract. Feed, digesta and faecal samples underwent analysis of Fe, Zn, and Cu and total tannins (TT). Goats had significantly higher reticulum Cu concentrations expressed on DM as compared to sheep. Faecal Cu concentrations were higher for goats compared to sheep. Reticulum and colon digesta Zn levels were higher in goats than sheep. Abomasum and colon Fe levels were higher in sheep than goats when expressed on DM. These results suggest differences in feed intake, micromineral absorption, secretion and excretion between sheep and goats, pointing to a divergent mineral metabolism as an adaptation to the challenge of a dry season diet having very low nutritive value.

Incubation of selected fermentable fibres with feline faecal inoculum: correlations between in vitro fermentation characteristics and end products.

This study aimed to evaluate correlations between fermentation characteristics and end products of selected fermentable fibres (three types of fructans, citrus pectin, guar gum), incubated with faecal inocula from donor cats fed two diets, differing in fibre and protein sources and concentrations. Cumulative gas production was measured over 72 h, fermentation end products were analysed at 4, 8, 12, 24, 48 and 72 h post-incubation, and quantification of lactobacilli, bifidobacteria and bacteroides in fermentation liquids were performed at 4 and 48 h of incubation. Partial Pearson correlations, corrected for inoculum, were calculated to assess the interdependency of the fermentation characteristics of the soluble fibre substrates. Butyric and valeric acid concentrations increased with higher fermentation rates, whereas acetic acid declined. Concentrations of butyric acid (highest in fructans) and propionic acid were inversely correlated with protein fermentation end products at several time points, whereas concentrations of acetic acid (highest in citrus pectin) were positively correlated with these products at most time points. Remarkably, a lack of clear relationship between the counts of bacterial groups and their typically associated products after 4 h of incubation was observed. Data from this experiment suggest that differences in fibre fermentation rate in feline faecal inocula coincide with typical changes in the profile of bacterial fermentation products. The observed higher concentrations of propionic and butyric acid as a result of fibre fermentation could possibly have beneficial effects on intestinal health, and may be confounded with a concurrent decrease in the production of putrefactive compounds. In conclusion, supplementing guar gum or fructans to a feline diet might be more advantageous compared with citrus pectin. However, in vivo research is warranted to confirm these conclusions in domestic cats.