ABSTRACT
The effects of vaccination on avian blood parameters are poorly understood. The present study was designed to evaluate whether different strains (Ulster 2C, B1, live LaSota and inactivated LaSota) of Newcastle disease vaccines had an effect on the haematological profile of female turkeys. Seventy-five female turkeys were allocated to treatment groups according to vaccination strain. All the birds, except those in the control group, were vaccinated at 32 weeks of age and revaccinated at 40 and 48 weeks of age. Blood samples were obtained for haematological analyses and serum samples for the haemagglutination inhibition test. Haemoglobin concentration was significantly lower (p < 0.05) in vaccinated female turkeys than in the control birds 28 days after vaccination. Monocytes were significantly higher (p < 0.05) in 44-week-old female turkeys vaccinated with inactivated LaSota strain compared with the other groups. Turkeys vaccinated with the B1 strain showed significantly higher (p < 0.05) total white blood cell counts compared with the other groups vaccinated with various commercial strains of the Newcastle disease virus. In conclusion, female turkeys showed significant differences in haemoglobin concentrations, monocytes and white blood cell counts when vaccinated against Newcastle disease.
Subject(s)
Newcastle Disease/prevention & control , Turkeys/blood , Viral Vaccines/immunology , Aging , Animals , Antibodies, Viral/blood , Female , Vaccines, Inactivated/immunologyABSTRACT
The effects of vaccination on avian blood parameters are poorly understood. The present study was designed to evaluate whether different strains (Ulster 2C, B1, live LaSota and inactivated LaSota) of Newcastle disease vaccines had an effect on the haematological profile of female turkeys. Seventy-five female turkeys were allocated to treatment groups according to vaccination strain. All the birds, except those in the control group, were vaccinated at 32 weeks of age and revaccinated at 40 and 48 weeks of age. Blood samples were obtained for haematological analyses and serum samples for the haemagglutination inhibition test. Haemoglobin concentration was significantly lower (p < 0.05) in vaccinated female turkeys than in the control birds 28 days after vaccination. Monocytes were significantly higher (p < 0.05) in 44-week-old female turkeys vaccinated with inactivated LaSota strain compared with the other groups. Turkeys vaccinated with the B1 strain showed significantly higher (p < 0.05) total white blood cell counts compared with the other groups vaccinated with various commercial strains of the Newcastle disease virus. In conclusion, female turkeys showed significant differences in haemoglobin concentrations, monocytes and white blood cell counts when vaccinated against Newcastle disease.
ABSTRACT
Este experimento foi realizado para avaliar o emprego da microscopia eletrônica de varredura no estudo da reaçäo respiratória pós-vacinal em epitélio traqueal de codornas (Coturnix coturnix japonica) imunizadas contra a doença de Newcastle. Foram utilizadas 36 codornas que foram distribuídas em quatro grupos, sendo: T1 - grupo de aves controle (näo vacinado), T2 - grupo de aves vacinadas com a estirpe Ulster 2C, T3 - grupo vacinado com a estirpe B, T4 - grupo de aves vacinadas com a estirpe LaSota. Independente do grupo experimental, as aves näo apresentaram sinais clínicos detectáveis de reaçäo respiratória pós-vacinal. Entretanto, na análise de fragmentos traqueais, ao microscópio eletrônico de varredura, observou-se que as codornas vacinadas com as estirpes B e LaSota desenvolveram descamaçäo epitelial da traquéia, enquanto as aves vacinadas com a estirpe Ulster 2C näo desenvolveram tal alteraçäo, mostrando um epitélio traqueal íntegro, semelhante ao grupo controle.
Subject(s)
Coturnix , Newcastle Disease/prevention & control , Poultry Diseases/prevention & control , Viral Vaccines , Microscopy, Electron, Scanning/veterinaryABSTRACT
This study aimed to evaluate the effect of dietary ochratoxin, in the presence or absence of aluminosilicate, on the histology of the bursa of Fabricius, liver and kidneys, and on the humoral immune response of broilers vaccinated against Newcastle disease virus. The exposure of birds to 2 p.p.m. ochratoxin, in the presence or absence of aluminosilicate, reduced their humoral immune response and the number of mitotic cells in the bursa. The relative weight of the livers of the birds exposed to this toxin was increased and, microscopically, there was hepatocyte vacuolation and megalocytosis with accompanying hyperplasia of the biliary epithelium. The kidneys showed hypertrophy of the renal proximal tubular epithelium, with thickening of the glomerular basement membrane. Aluminosilicate did not ameliorate the deleterious effects of the ochratoxin.
