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1.
J Neurol Sci ; 119(1): 38-42, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8246009

ABSTRACT

In Duchenne muscular dystrophy, the progression of the disease is always severe and predictable, while in Becker dystrophy there is a wide variability (intra and inter familial) in the severity of the phenotype. We report here a family in which the proband, who is currently 15 years old, is showing a severe DMD progression, while his affected maternal uncle, aged 29, has a more benign course, compatible with BMD. No DNA deletion was detected in both patients. Dystrophin analysis through immunofluorescence and western blotting showed a negative pattern in the youngest patient and a positive one in the oldest. Apparently, this is the first report on intrafamilial variability in dystrophin abundance correlated with a difference in the severity of the phenotype.


Subject(s)
Dystrophin/metabolism , Membrane Proteins , Muscular Dystrophies/metabolism , Adolescent , Adult , Blotting, Western , Creatine Kinase/metabolism , Cytoskeletal Proteins/chemistry , Cytoskeletal Proteins/metabolism , DNA/analysis , Dystrophin/chemistry , Dystrophin/genetics , Family , Female , Fluorescent Antibody Technique , Humans , Immunohistochemistry , Male , Muscles/chemistry , Muscles/pathology , Muscular Dystrophies/genetics , Phenotype , Polymerase Chain Reaction , Utrophin
4.
J Neurol Sci ; 103(1): 65-75, 1991 May.
Article in English | MEDLINE | ID: mdl-1865235

ABSTRACT

The differential clinical diagnosis between the X-linked muscular dystrophies (DMD and BMD) and autosomal recessive limb-girdle muscular dystrophy (LGMD), which is extremely important for genetic counseling, may be very difficult. The aim of the present report is to describe clinical and laboratory findings in patients from large families, with AR inheritance, in an attempt to characterize better cases which have been diagnosed as LGMD compared with the X-linked forms. The main features analysed are: age of onset and of confinement to a wheelchair, reproductive performance, serum enzymes (CK and PK) and dystrophin assessment (through immunohistochemistry and Western blot). Twenty-two families, with 62 affected patients diagnosed as limb-girdle muscular dystrophy, were included in this report. In 19 families, the patients had a milder clinical course, while in the remaining 3, the progression of the disease was continuous and clinically similar to X-linked DMD ("DMD-like"). A high consanguinity rate was observed among the parents of the affected patients (77%). No major clinical difference was observed between the X-linked and the AR forms. However, muscle dystrophin was found qualitatively and quantitatively normal in the autosomal forms but absent or abnormal in the X-linked ones. The reproductive performance was significantly higher for male than female patients. In addition, a surprising finding was the significantly greater fitness estimated for male LGMD cases as compared with Becker patients of comparable age studied in our center. The implications of such findings are discussed.


Subject(s)
Muscular Dystrophies/diagnosis , Muscular Dystrophies/genetics , Adolescent , Adult , Brazil , Child , Child, Preschool , Creatine Kinase/blood , Diagnosis, Differential , Female , Genes, Recessive , Genetic Linkage , Humans , Male , Middle Aged , Muscles/pathology , Muscular Dystrophies/enzymology , Muscular Dystrophies/pathology , Pedigree , Pyruvate Kinase/blood , Syndrome , X Chromosome
5.
J Neurol Sci ; 102(2): 190-6, 1991 Apr.
Article in English | MEDLINE | ID: mdl-2072118

ABSTRACT

Serum creatine-kinase (CK) activities were determined in 536 patients affected with X-linked muscular dystrophy (456 with Duchenne or DMD and 80 with Becker or BMD) and serum pyruvate-kinase (PK) in 360 among them (309 DMD and 51 BMD). The aim of this investigation was to assess the variability and rate of decrease in serum activity in DMD as compared with BMD as a function of age and in DMD as a function of Vignos scale as well. In DMD, maximum CK and PK activities were found around 1-6 years old and the average rate of decline according to age was estimated as 0.18 per year and 0.27-0.29 for both enzymes as a function of Vignos scale (assessed in 291 cases). For BMD, maximum serum enzyme levels were found around 10-15 years old and the rate of decline of serum activity per year was 0.06 for CK and 0.07 for PK. If maximum levels of serum enzyme reflect active muscle degeneration and the rate of decline per year to progressive loss of muscle mass (responsible for the release of muscle enzymes to the blood stream) our observations suggest: (a) active muscle degeneration occurs, on average, 5 years later in the group of outliers and 10 years later in BMD as compared with severe DMD; (b) the rate in which muscle mass is lost is significantly greater in DMD than in BMD and therefore serum enzyme determinations may represent an important test for evaluation of therapeutic trials; (c) serum enzymes determination may represent an important preliminary test to discriminate in a proportion of young patients if they will develop a severe or milder phenotype.


Subject(s)
Creatine Kinase/blood , Muscular Dystrophies/enzymology , Pyruvate Kinase/blood , Adolescent , Adult , Age Factors , Child , Child, Preschool , Dystrophin/analysis , Humans , Infant , Muscular Dystrophies/blood , Muscular Dystrophies/classification , Predictive Value of Tests , Regression Analysis , Severity of Illness Index
6.
Am J Med Genet ; 35(1): 22-7, 1990 Jan.
Article in English | MEDLINE | ID: mdl-2301469

ABSTRACT

Seventy-five male and 50 female students from 2 special schools for mildly, moderately retarded, or borderline individuals were screened clinically and cytogenetically in order to estimate the contribution of fragile X [fra(X)] syndrome to the cause of mental retardation in Brazil. We found 6 males (8%) from 4 families and 2 unrelated females (4%) with fra(X) chromosomes. One male and one female were isolated cases. The estimated frequency of Martin-Bell [fra(X)] syndrome among mentally impaired individuals in Brazil was similar to that previously reported in other countries.


Subject(s)
Fragile X Syndrome/complications , Adolescent , Adult , Brazil , Child , Chromosome Banding , Female , Genetic Testing , Humans , Intellectual Disability/complications , Karyotyping , Male , Pedigree , Sex Chromosome Aberrations
7.
Am J Med Genet ; 31(4): 821-33, 1988 Dec.
Article in English | MEDLINE | ID: mdl-3239574

ABSTRACT

Human growth hormone (HGH) inhibition may be beneficial in Duchenne muscular dystrophy (DMD) and slow down the rate of progression of the disease. The purposes of the present investigation were: 1) to assess, through pharmacological stimuli (L-dopa test), the HGH response in untreated DMD patients, and 2) to evaluate the inhibitory effect of mazindol on HGH levels as a potential treatment for DMD. In 55 DMD patients, HGH levels were measured through the L-dopa test, and 40 received mazindol. After 1 year, there was wide variability in the individual response to mazindol. An apparent diminution in the mean HGH level was observed in the whole group of patients; this was statistically significant after 3 and 6 months but not after 9 and 12 months of treatment. The results suggest that this drug is not effective for arresting growth or inhibiting HGH secretion for a prolonged period of time.


Subject(s)
Growth Hormone/blood , Indoles/pharmacology , Mazindol/pharmacology , Muscular Dystrophies/blood , Adolescent , Child , Child, Preschool , Humans , Levodopa , Male , Mazindol/adverse effects , Mazindol/therapeutic use , Muscular Dystrophies/drug therapy
8.
Am J Med Genet ; 29(4): 845-9, 1988 Apr.
Article in English | MEDLINE | ID: mdl-3400728

ABSTRACT

We report the results of a study performed in a sample of women with the Mayer-Rokitansky-Küster (MRK) anomaly and in their first-degree relatives. Our results are compatible with a traditional model of multifactorial determination; however, we cannot exclude the hypothesis of autosomal dominant inheritance, with an intermediate degree of penetrance and a highly variable expressivity of a single mutant gene. In this sense, our data seem to support the idea expressed recently by Opitz [1987].


Subject(s)
Abnormalities, Multiple/genetics , Kidney/abnormalities , Female , Humans , Pedigree
9.
Am J Med Genet ; 29(2): 405-10, 1988 Feb.
Article in English | MEDLINE | ID: mdl-3354613

ABSTRACT

We have evaluated the relation between height and rate of clinical progression in boys with Duchenne muscular dystrophy (DMD). In all, 111 DMD patients with age ranging from 2 to 23 years (mean 8.2 +/- 3.4 years) were assessed; of these patients, 92 had their height measured. Clinical course was determined through Vignos scale of functional disability, motor ability, and timed functional tests. All patients had grossly elevated serum creatine-kinase (CK) and pyruvate-kinase (PK) levels. When height was adjusted for patients' age, a statistically significant correlation was found between height and clinical course (positive with Vignos scale and negative with motor ability), suggesting that smaller boys have a better clinical course than taller patients of comparable age. These results support our previous hypothesis and suggest that growth inhibition seems to be effective in diminishing the progression of DMD.


Subject(s)
Muscular Dystrophies/pathology , Age Factors , Body Weight , Growth Disorders/complications , Humans , Motor Activity , Muscular Dystrophies/physiopathology
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