ABSTRACT
BACKGROUND: The combination of zinc oxide (ZnO) nanoparticles (NPs) with carriers enhances the anticancer effect of nanocomposites. AIM: To explore the mechanisms of cytotoxic action of dextran-graft-polyacrylamide (D-g-PAA/ZnO) NPs against prostate cancers cell lines in vitro. MATERIALS AND METHODS: Dextran-polyacrylamide was used as a matrix for the synthesis of ZnO NPs. Prostate cancer cells LNCaP, DU-145 and PC-3 were treated with D-g-PAA/ZnO NPs. The expression of Bax, Bcl-2, p53 and Ki-67 was studied using immunocytochemical analysis. Cytomorphological changes in cells were detected after their incubation with nanocomposites for 24 h. RESULTS: The treatment with D-g-PAA/ZnO NPs caused the increase in the Bax and p53 and the decrease in Ki-67 and Bcl-2 expression. Morphological changes associated with apoptosis were registered: decrease in cell size, appearance of cytoplasmic vacuolation, condensation of chromatin, blebbing. CONCLUSIONS: Treatment with D-g-PAA/ZnO nanocomposite led to the initiation of apoptotic cell death in prostate cancer cells in vitro.
Subject(s)
Metal Nanoparticles , Prostatic Neoplasms , Zinc Oxide , Male , Humans , Zinc Oxide/pharmacology , Dextrans/metabolism , Dextrans/pharmacology , bcl-2-Associated X Protein/metabolism , bcl-2-Associated X Protein/pharmacology , Tumor Suppressor Protein p53/metabolism , Ki-67 Antigen/metabolism , Reactive Oxygen Species/metabolism , Apoptosis , Proto-Oncogene Proteins c-bcl-2 , Cell LineSubject(s)
Biopolymers/analysis , Ions/isolation & purification , Organic Chemicals/analysis , Specimen Handling/history , Spectrometry, Mass, Electrospray Ionization/history , Atmospheric Pressure , Biopolymers/chemistry , Biopolymers/history , Equipment Design/history , Equipment Failure Analysis , History, 21st Century , Ions/chemistry , Ions/history , Organic Chemicals/chemistry , Organic Chemicals/history , Solutions , Specimen Handling/instrumentation , Specimen Handling/methods , Spectrometry, Mass, Electrospray Ionization/instrumentation , Spectrometry, Mass, Electrospray Ionization/methods , TranslationsABSTRACT
The effect of the type I interferon on the development and process of experimental pyelonephritis caused by E. coli was studied on mice weighing 12 to 14 g. Interferon was administered intraperitoneally in a dose of 1000 units on days 3 and 7 of the disease. It was shown that the administration of the type I interferon to the mice with experimental pyelonephritis promoted rapid elimination of bacteria from the kidneys, prevented their penetration to the contralateral (intact) kidney, prevented marked macro- and microscopic damages in the kidneys, lowered the intensity of the inflammatory reaction, and increased the phagocytic activity of neutrophils and the number of the E-rosette-forming lymphocytes in the thymus. The data provided experimental grounding for clinical trials of interferon preparations in treatment of bacterial pyelonephritis.
Subject(s)
Escherichia coli Infections/therapy , Interferon Type I/therapeutic use , Pyelonephritis/therapy , Adjuvants, Immunologic , Animals , Escherichia coli/drug effects , Escherichia coli Infections/immunology , Escherichia coli Infections/microbiology , Kidney Pelvis/drug effects , Kidney Pelvis/immunology , Kidney Pelvis/microbiology , Mice , Phagocytosis/drug effects , Phagocytosis/immunology , Pyelonephritis/immunology , Pyelonephritis/microbiology , Rosette Formation , T-Lymphocytes/drug effects , T-Lymphocytes/immunologyABSTRACT
In experiments on mice the influence of mouse serum interferon, type I, on immune response in pyelonephritis caused by staphylococci and P. aeruginosa has been studied. The immunomodulating action of interferon and its therapeutic effectiveness have been shown to depend on the etiology of the disease. When injected intraperitoneally in a dose of 1,000 ED, interferon produces a pronounced therapeutic effect in pyelonephritis caused by P. aeruginosa and no effect in pyelonephritis of staphylococcal etiology. Type I interferon introduced in the dose used in this investigation has no influence on the killer activity of spleen lymphocytes, enhances the activity of the complement and the production of antibodies, produces a leukopenic effect and, depending on the etiology of pyelonephritis, exerts influence on the activity of dehydrogenases, the number of EAC- and E-rosette-forming cells, the oxidation metabolism of neutrophils and their phagocytic activity.
Subject(s)
Interferon Type I/therapeutic use , Pseudomonas Infections/therapy , Pyelonephritis/therapy , Staphylococcal Infections/therapy , Animals , Male , Mice , Phagocytosis/drug effects , Pseudomonas Infections/immunology , Pyelonephritis/etiology , Pyelonephritis/immunology , Species Specificity , Staphylococcal Infections/immunologyABSTRACT
Experimental investigations indicate that localized forms of staphylococcal, Pseudomonas aeruginosa and associated (Pseudomonas aeruginosa-staphylococcal) infection inhibit the lysozyme activity, cause disorders in the metabolism of P-450 cytochrome, free-radical and iron-containing liver proteins.
Subject(s)
Liver/metabolism , Muramidase/blood , Pseudomonas Infections/metabolism , Staphylococcal Infections/metabolism , Animals , Cytochrome P-450 Enzyme System/metabolism , Free Radicals , Iron/metabolism , Male , Metalloproteins/metabolism , Mice , Time FactorsSubject(s)
Cholangitis/immunology , Cholecystitis/complications , Peritonitis/immunology , Acute Disease , Aged , B-Lymphocytes/immunology , Cholangitis/etiology , Cholecystitis/immunology , Female , Humans , Immune Tolerance , Immunoglobulins/analysis , Male , Middle Aged , Peritonitis/etiology , T-Lymphocytes/immunologySubject(s)
Pseudomonas Infections/blood , Staphylococcal Infections/blood , Animals , Blood Cell Count , Mice , Time FactorsABSTRACT
The Soviet-made mass-spectrometer, model MX-6202 has a number of features that distinguish it from foreign-made devices. The experience with its operation suggests it to be a promising piece of equipment in evaluating the state of gas exchange in the organism, the one that will find wide application in the medical practice. The progress of the mass-spectrometric technique clears the way for detailed, in-depth medical and medico-biological research.
