Genetic catalepsy and ultralow dose antibodies to S-100B antigen.

We studied consumption of 20% sucrose solution by rats genetically predisposed to catalepsy (GC strain) during training. The consumption of sucrose solution by GC rats was lower in comparison to that in Wistar rats. "Potentiated" antibodies to S-100B antigen administered orally after training sessions increased the number and duration of subsequent contacts of rats with sucrose solution.

Effect of antibodies against S-100B antigen in ultralow doses on sucrose consumption during learning.

We studied the effect of potentiated antibodies against S-100B antigen on 20% sucrose consumption by Wistar rats under conditions of free-choice drinking from the bowls with sucrose and water during presentation of an acoustic pre-nociceptive or neutral signal. Peroral administration of antibodies after training sessions increased the number and duration of contacts with sucrose solution.

Improvement of Memory by Means of Ultra-Low Doses of Antibodies to S-100B Antigen.

Antigen S-100B of nervous tissue, according to the data of numerous studies, affects the mechanisms of nervous system plasticity and memory. The influence of ultralow doses of antibodies to S-100B (6C dilution, according to the homeopathic pharmacopoeia) has been studied on three learning behavioral models on Wistar rats, which were inhibitory avoidance, choosing of bowls with sucrose and feeding behavior cessation after auditory signal. For all three tasks, parameters of reproduction of the learned skills improved after per oral administration of potentiated antibodies to S-100B antigen immediately after learning. Possible mechanisms of the anti-S-100B antibodies influence on memory formation are discussed.

Effect of ultralow doses of antibodies to S-100 antigen (Proproten-100) on spatial learning in rats.

In experiments on rats we studied the effect of potentiated antibodies against S-100 antigen on training a step-down passive avoidance task and choice between drinking bowls with sucrose solution. Peroral treatment with antibodies accelerated inhibition of ineffective and punished locomotor reactions in animals.

Effects of antibodies against S-100 antigen in ultralow doses (Proproten-100) on acquisition of avoidance response in rats.

We studied the effect of potentiated antibodies against S-100 antigen on learning of avoidance responses of 2 types. Peroral administration of antibodies promoted inhibition of locomotor activity and feeding behavior, which was associated with electrical pain stimulation. Our results indicate that the preparation in ultralow doses modulates the mechanism of memory formation.

Behavioral effects of potentiated antibodies to morphine and mu-opioid receptors during withdrawal syndrome.

We studied the effects of potentiated antibodies to morphine and mu-opioid receptors on animal behavior in the forced swimming test and reaction of light avoidance after morphine withdrawal. Antibodies to morphine normalized behavioral characteristics in the forced swimming test, while antibodies to mu-opioid receptors reduced dark preference in animals.

Morphine and antibodies to mu-opiate receptors in ultralow doses: effect on oxygen consumption.

The degree of oxygen consumption in rats was determined after intraperitoneal injection of morphine in a single dose of 5 mg/kg. Some animals were injected with morphine and perorally received morphine or antibodies to mu-opiate receptors in ultralow doses obtained by the technology of potentiation. Potentiated substances significantly reduced oxygen consumption intensified after morphinization. Potentiated morphine decreased the intensity of oxygen consumption to the level observed in intact animals. Our results indicate that morphine in ultralow doses modulates the action of this compound in toxic concentrations (bipathic phenomenon) and produces the normalizing effect. Potentiated antibodies to mu-opiate receptors modulated the effect of morphine, which indicates that they are involved in the development of opium dependence.

[Changes in the content of water-soluble brain proteins-antigens during bilateral avoidance training of rats]. / Izmenenie soderzhaniia vodorastvorimykh belkov-antigenov mozga pri obuchenii krys dvustoronnemu izbeganiiu.

The levels of water-soluble brain antigens have been studied during bilateral avoidance learning in Wistar rats. With the help of cross and rocket immunoelectrophoresis the content of antigens was measured in the brain structures an hour and a week after training. It was shown that the content of one of six antigens in the auditory cortex correlated with the number of tone-shock combinations. The antigen was not found in the liver and its amount was higher in the brain stem, as compared to cerebral cortex and cerebellum. The results suggest that this antigen may be involved in the processes of memory build-up.

[Aggression and learning in a strain of rats predisposed to catalepsy]. / Agressiia i obuchenie u linii krys, predraspolozhennykh k katalepsii.

Noradrenaline content is lower in the diencephalon of rats with genetic predisposition to catalepsy as compared to control Wistar population. Besides, there is a statistically non significant tendency to a lower noradrenaline level in the striatum and hippocampus. Pain stimulus induces in cataleptic rats a smaller decrease in noradrenaline than in Wistar. The shock-induced aggression is higher in cataleptic rats than in Wistar. All these data point to a lower noradrenergic activity in rats with genetic predisposition to catalepsy. In cataleptic rats, both the development and extinction of the conditioned avoidance reaction is slower than in Wistar. The changes of noradrenergic activity and learning are similar to those described in chronic amphetamine intoxication. It is supposed that these changes in learning are caused by increased serotonergic (found earlier) and decreased noradrenergic activity.

[Effect of D,L-dopa on the content of protein antigens in rat brain structures]. / Vliianie D,L-DOFA na soderzhanie belkov-antigenov v nekotorykh strukturakh mozga krys.

Cross immunoelectrophoresis was used to study the effect of administering the noradrenaline precursor D,L-DOPA on the content of water-soluble antigens in the hypothalamus, cerebellum and frontal cortex of rats. The content of 3 out of the 10 antigens under study significantly increased in the hypothalamus and that of 2 in the cerebellum after intraperitoneal injection of D,L-DOPA. The content of these antigens was greater in the structures with a high noradrenaline level.

[Neurospecific antigen D in the postnatal ontogeny of rat brain structures]. / Neirospetsificheskii antigen D v postnatal'nom ontogeneze struktur mozga krys.

The time course of antigen D similar to well-known neurospecific proteins 14-3-2 and antigen alpha was studied during postnatal ontogenesis of brain structures of Wistar rats: Cross immunoelectrophoresis was made use of to measure the content of antigen D in the visual cortex, hippocamp and nucleus caudatus of rats aged 7, 14, 21 and 30 days and 1 year. Changes in the levels of two antigen D components, slow- and fast-migrating (SMC and FMC) were recorded to show that their content in the test structures increased most intensely from the 14th to the 21st day of postnatal development. In all the brain structures of tendency was recorded towards the increased SMC/FMC ratio during postnatal ontogenesis, that may point to an important role of FMC for bain functions in maturing and adult animals.