ABSTRACT
Hand, foot and mouth disease (HFMD) is a paediatric disease associated with enteroviruses (EVs). Among EVs, coxsackievirus A-16 (CVA-16) strain is currently in circulation and causing outbreaks in India. Neonatal mice (Institute of Cancer Research) strains were infected with CVA-16 strain isolated from HFMD patients to conduct pathological and molecular studies. Infected organs were harvested as per time points. A real-time polymerase chain reaction was used for qualitative estimation of viral RNA in organ tissues of infected mice. Skeletal muscle, brain tissue and cardiac tissues were the major target sites of CVA-16 tropism. The first-ever study was conducted on CVA-16 strains using the current approach in India.
Subject(s)
Coxsackievirus Infections/virology , Enterovirus/genetics , RNA, Viral/analysis , Real-Time Polymerase Chain Reaction , Animals , Animals, Newborn , Child, Preschool , Coxsackievirus Infections/diagnosis , Enterovirus/isolation & purification , Enterovirus/pathogenicity , Feces/virology , Humans , India , Infant , Mice , Mice, Inbred ICR , Pharynx/virology , Rectum/virologyABSTRACT
An encephalitis outbreak in 2003 in children from India was attributed to Chandipura virus. Sequence analyses of G, N, and P genes showed 95.6%-97.6% nucleotide identity with the 1965 isolate (G gene, 7-11 amino acid changes); N and P genes were highly conserved.
