ABSTRACT
The keton bodies are created generally in a liver, but this organ has no enzymatic system able to metabolize them. A dependence exists between the molar acetoacetate concentration and the molar beta-hydroxybutyrate concentration, and it can be an indicator of quantitive relations between NAD and NADH. Metabolic reserve can be estimated on the basis of the molar concentration of keton bodies in the arterial blood--of the AKBR value. Tests were carried out on specially prepared rabbit arterial blood samples. Because there is no way to measure NAD to NADH cocentrations in the liver directly a relationship between acetoacetate and beta-hydroxybutarate serves to estimate of the oxydo-reductive status of this key organ. That is why AKBR values seem to be extremely important. Vitamin E is known for it's cytoprotective influence of hepatic cells damage. In the presented paper we've challenged this hypothesis by analysing of correlation between AKBR values in examined rabbit groups.
Subject(s)
Ketone Bodies/blood , Liver Diseases/veterinary , Rabbits/blood , Animals , Carbon Tetrachloride/toxicity , Chemical and Drug Induced Liver Injury , Female , Homeostasis , Liver Diseases/blood , Liver Diseases/drug therapy , Male , Oxidation-Reduction , Vitamin E/pharmacologyABSTRACT
The aim of our research was estimation of the activity of superoxide dysmutase (SOD) and glutathione peroxidase (GPx) in rabbits' blood after administration of single dose of carbon tetrachloride (CCl4) and synthetic analogue PGE1 - Cytotec. The research was carried out on 56 Chinchilla rabbits. By means of stomach tube rabbits were administered respectively: CCl4, Cytotec, CCl4 with Cytotec, indomethacin, Cytotec with indomethacin and CCl4 with Cytotec and indomethacin simultneously. In comparison with control group almost double increase of SOD activity was stated at rabbits which were administered CCl4 and CCl4 with indomethacin. At rabbits which were administered CCl4 with Cytotec and CCl4 with Cytotec and with indomethacin, the SOD activity increased by only 50% in comparison with control group. At rabbits in whose blood the SOD activity increased, the activity of GPx decreased on average by respectively 40% and 15%. The stated changes of SOD and GPx activity in rabbits' blood which were administered prostaglandin apart from CCl4 indicates prostaglandin's indirect interference into hepatocytes antioxidative system and thus its hepatoprotective activity.