[P21-ras protein in tumors of the large intestine]. / Bialko p21-ras w rakach jelita grubego.

We carried out a prospective analysis of cytoplasmatic accumulation of p21ras protein-a Ki-ras gene product. The study was completed on the group of 80 patients with sporadic colorectal cancer. p21ras protein was detected immunohistochemically with use of NCC-001 antibody. In 64/80 cases (80%) we found p21ras protein accumulation in the tumor. We found that the protein accumulation is present more often in poorly differentiated cancers 91.3% (42/46) than in well and intermediate differentiated 64.7% (22/34) and in the right side of the colon 100% (14/14) than in left side colon 53.3% (14/24). The differences were statistically significant (p < 0.05). We found no statistically significant differences in survival time after surgery in correlation with p21ras accumulation in tumor. Our result suggest that p21ras accumulation is not a solitary prognostic factor in sporadic colorectal cancer.

[The role of nm23 protein in progression of sporadic adenocarcinoma of the large intestine]. / Udzial bialka nm23 w rozwoju sporadycznych gruczolakoraków jelita grubego.

We analysed the nm23 protein expression in the selected group of 80 patients with sporadic colorectal cancer. nm23 protein was detected in cytoplasm with use of monoclonal anti nm23 antibody. Decreased expression of nm23 protein was found in 58/80 (72.5%) colorectal cancer samples. Positive reaction was found more often in the group A and B according to Dukes, than in the C and D. The difference was statistically significant at p < 0.05, and the occurrence was 75% vs. 15.63% respectively. We also found statistically significant difference in nm23 protein expression in well differentiated (G1) and poorly (G3) and intermediate (G2) differentiated. The positive reaction was observed in 85.71% in G1 group and 12.92% in G2 and G3 together. On the base of our study we suggest a usefulness of histochemical identification of nm23 protein expression in evaluation of metastatic potential of colorectal cancer.

[P53 protein in adenocarcinoma of the large intestine]. / Bialko p53 w gruczolakorakach jelita grubego.

P53 gen mutations play significant role in neoplastic transformation of colorectal mucosa. We investigated p53 immunostaining in 80 cases of spontaneous human colorectal adenocarcinomas (with monoclonal DO7 antibody and LSAB+ kit). We found positive, nuclear p53 immunostaining in 64% of nonmucinous adenocarcinoma tissues and in 19% of mucinous adenocarcinomas tissues. P53 protein deposits were most often found in colorectal adenocarcinomas localised in rectum (66.67%) and in advanced (Dukes C, D) colorectal adenocarcinomas (59.38%) as well. There was no statistical significance between the p53 positive immunostaining and the histological differentiation of the colorectal adenocarcinomas. The overall survival of patients with tumours positive for p53 protein was significantly shorter than that of patients with colorectal cancers negative for p53 protein. We conclude that p53 immunohistochemical analysis may be treated as a supplementary prognostic marker for patients with colorectal adenocarcinoma, especially it may be useful for adjuvant therapy selection.

[Morphologic and toxicologic evaluation of response to subcutaneous para-osseous implants Medpol-1 and Medpol-2 in rats]. / Ocena morfologiczna i toksykologiczna wplywu podskórnych przykostnych wszczepów Medpol-1 i Medpol-2 na organizm szczura.

Cobalt alloys produced in Poland and meant for orthopedic implants were morphologically and toxicologically evaluated. They were implanted subcutaneously in the para-osseous region of rats' heads. The results of the experimental tests strongly suggest the advisability of a clinical research project (study) on the examined cobalt alloys: Medpol-1 and Medpol-2.