Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Virol ; 80(9): 4447-57, 2006 May.
Article in English | MEDLINE | ID: mdl-16611905

ABSTRACT

Recombinant vesicular stomatitis virus (rVSV) vectors offer an attractive approach for the induction of robust cellular and humoral immune responses directed against human pathogen target antigens. We evaluated rVSV vectors expressing full-length glycoprotein D (gD) from herpes simplex virus type 2 (HSV-2) in mice and guinea pigs for immunogenicity and protective efficacy against genital challenge with wild-type HSV-2. Robust Th1-polarized anti-gD immune responses were demonstrated in the murine model as measured by induction of gD-specific cytotoxic T lymphocytes and increased gamma interferon expression. The isotype makeup of the serum anti-gD immunoglobulin G (IgG) response was consistent with the presence of a Th1-CD4+ anti-gD response, characterized by a high IgG2a/IgG1 IgG subclass ratio. Functional anti-HSV-2 neutralizing serum antibody responses were readily demonstrated in both guinea pigs and mice that had been immunized with rVSV-gD vaccines. Furthermore, guinea pigs and mice were prophylactically protected from genital challenge with high doses of wild-type HSV-2. In addition, guinea pigs were highly protected against the establishment of latent infection as evidenced by low or absent HSV-2 genome copies in dorsal root ganglia after virus challenge. In summary, rVSV-gD vectors were successfully used to elicit potent anti-gD Th1-like cellular and humoral immune responses that were protective against HSV-2 disease in guinea pigs and mice.


Subject(s)
Herpes Simplex Virus Vaccines/immunology , Herpesvirus 2, Human/immunology , Th1 Cells/immunology , Vagina/immunology , Vagina/virology , Vesicular stomatitis Indiana virus/genetics , Viral Envelope Proteins/immunology , Animals , Antibody Formation/immunology , Female , Genetic Vectors/genetics , Glycoproteins/genetics , Glycoproteins/immunology , Glycoproteins/metabolism , Guinea Pigs , Herpes Simplex Virus Vaccines/genetics , Herpesvirus 2, Human/genetics , Herpesvirus 2, Human/metabolism , Mice , Models, Animal , Viral Envelope Proteins/genetics , Viral Envelope Proteins/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...