Especialização em Educação Popular em Saúde na Promoção de Territórios Saudáveis e Sustentáveis da Fiocruz: uma análise sob a ótica dos próprios educandos / Specialization in Popular Health Education in the Promotion of Healthy and Sustainable Territories at Fiocruz: an analysis from the students' perspective

RESUMO Este estudo objetivou compreender a experiência do curso de Especialização em Educação Popular em Saúde na Promoção de Territórios Saudáveis e Sustentáveis da Fundação Oswaldo Cruz (Fiocruz), sob a ótica dos educandos. Foi realizada uma pesquisa qualitativa por meio de entrevistas semiestrutu-radas feitas com estudantes do curso (n = 20). Os dados foram interpretados pela análise de conteúdo temática de Bardin. Os resultados compuseram três categorias temáticas: a Pedagogia da Formação, que apresenta a compreensão das estratégias formativas e metodológicas do curso; a Pedagogia do Encontro, que traz as potências que se fizeram no encontro entre as diversidades; a Pedagogia do Movimento, que levanta as implicações do curso para a transformação pessoal e profissional dos participantes. A formação apresentou-se como um potente instrumento de transformação de perspectivas e de práticas, em que o encontro atuou como via de articulação política e de cuidado à saúde.

ABSTRACT This study aimed to understand the experience of the Specialization course in Popular Health Education in the Promotion of Healthy and Sustainable Territories at Oswaldo Cruz Foundation (Fiocruz), from the student's perspective. A qualitative research was carried out through semi-structured interviews with students from the course (n = 20). Data were interpreted by Bardin's thematic content analysis. The results comprised three thematic categories: the Pedagogy of Training, which presents the understanding of the training and methodological strategies of the course; the Pedagogy of the Encounter, which brings the powers that have been made in the encounter between diversity; the Pedagogy of Movement, which raises the implications of the course for the personal and professional transformation of the participants. Training presented itself as a powerful instrument for transforming perspectives and practices, in which the meeting acted as a means of political articulation and health care.

Engineering buffer layers to improve temperature resilience of magnetic tunnel junction sensors.

Improving the thermal resilience of magnetic tunnel junctions (MTJs) broadens their applicability as sensing devices and is necessary to ensure their operation under harsh environments. In this work, we are address the impact of temperature on the degradation of the magnetic reference in field sensor stacks based on MgO-MTJs. Our study starts by simple MnIr/CoFe bilayers to gather enough insights into the role of critical morphological and magnetic parameters and their impact in the temperature dependent behavior. The exchange bias coupling field (Hex), coercive field (Hc), and blocking temperature (Tb) distribution are tuned, combining tailored growth conditions of the antiferromagnet and different buffer layer materials and stackings. This is achieved by a unique combination of ion beam deposition and magnetron sputtering, without vaccum break. Then, the work then extends beyond bilayers into more complex state-of-the-art MgO MTJ stacks as those employed in commercial sensing applications. We systematically address their characteristic fields, such as the width of the antiferromagnetic coupling plateau ΔH, and study their dependence on temperature. Although, [Ta/CuN] buffers showed higher key performance indications (e.g.Hex) at room temperature in both bilayers and MTJs, [Ta/Ru] buffers showed an overall wider ΔHup to 200 °C, more suitable to push high temperature operations. This result highlights the importance of properly design a suitable buffer layer system and addressing the complete MTJ behavior as function of temperature, to deliver the best stacking design with highest resilience to high temperature environments.

Abscisic Acid Synthesis and Signaling during the Ripening of Raspberry (Rubus idaeus 'Heritage') Fruit.

The raspberry (Rubus idaeus L.) fruit is characterized by its richness in functional molecules and high nutritional value, but the high rate of fruit softening limits its quality during postharvest. Raspberry drupelets have a particular ripening regulation, depending partially on the effect of ethylene produced from the receptacle. However, the possible role of abscisic acid (ABA) in the modulation of quality parameters during the ripening of raspberry is unclear. This study characterized the fruit quality-associated parameters and hormonal contents during fruit development in two seasons. The quality parameters showed typical changes during ripening: a drastic loss of firmness, increase in soluble solids content, loss of acidity, and turning to a red color from the large green stage to fully ripe fruit in both seasons. A significant increase in the ABA content was observed during the ripening of drupelets and receptacles, with the higher content in the receptacle of ripe and overripe stages compared to the large green stage. Moreover, identification of ABA biosynthesis-(9-cis-epoxycarotenoid dioxygenase/NCED) and ABA receptor-related genes (PYRs-like receptors) showed three genes encoding RiNCEDs and nine genes for RiPYLs. The expression level of these genes increased from the large green stage to the full-ripe stage, specifically characterized by a higher expression of RiNCED1 in the receptacle tissue. This study reports a consistent concomitant increase in the ABA content and the expression of RiNCED1, RiPYL1, and RiPYL8 during the ripening of the raspberry fruit, thus supporting the role for ABA signaling in drupelets.

The Omicron Lineages BA.1 and BA.2 (Betacoronavirus SARS-CoV-2) Have Repeatedly Entered Brazil through a Single Dispersal Hub.

Brazil currently ranks second in absolute deaths by COVID-19, even though most of its population has completed the vaccination protocol. With the introduction of Omicron in late 2021, the number of COVID-19 cases soared once again in the country. We investigated in this work how lineages BA.1 and BA.2 entered and spread in the country by sequencing 2173 new SARS-CoV-2 genomes collected between October 2021 and April 2022 and analyzing them in addition to more than 18,000 publicly available sequences with phylodynamic methods. We registered that Omicron was present in Brazil as early as 16 November 2021 and by January 2022 was already more than 99% of samples. More importantly, we detected that Omicron has been mostly imported through the state of São Paulo, which in turn dispersed the lineages to other states and regions of Brazil. This knowledge can be used to implement more efficient non-pharmaceutical interventions against the introduction of new SARS-CoV variants focused on surveillance of airports and ground transportation.

The use of health education strategies and art in rural health prevention focusing on alcohol abuse/addiction and other drugs.

INTRODUCTION: Health education is a relevant strategy to prevent alcohol and other drugs addiction. The objective of this study is to analyze health education strategies used to prevent drug abuse and addiction in rural areas. METHODS: This study is an integrative review. The study included articles indexed in Virtual Health Library, Periodicals Portal bases from CAPES, Brazilian Digital Library of Theses, PubMed, and SciELO. Relationships between health education strategies and art were sought, without obtaining satisfactory results. RESULTS: The selection of studies obtained 1173 articles. After exclusion, 21 publications were included in the sample. The predominant country of origin of articles was the USA, with 14 references. The lack of Latin American articles is highlighted. Overall, among the interventions carried out with a focus on preventing addiction to alcohol and other drugs, those that considered the specific cultural context of the studied community were relevant. Strategies adapted to the rural context must be built according to local values, beliefs, and practices. Motivational Interviewing proved to be an effective intervention for alcohol addiction harm reduction strategies. DISCUSSION: The frequency of harmful use of alcohol and other drugs in the rural population highlights the need to implement public policies focused on local communities. It is essential to adopt actions focused on health promotion. There is a need for further studies on health education strategies, including their relationships with the arts, in the context of preventing drug abuse in the rural population, in order to enable more effective interventions.

Atuação do fisioterapeuta nos Núcleos Ampliados de Saúde da Família e Atenção Básica no estado de Sergipe / The physiotherapist in the Expanded Family Health and Primary Care Centers of Sergipe

O objetivo é analisar as características socioeconômicas, de qualificação e a atuação dos fisioterapeutas nos Núcleos Ampliados de Saúde da Família e Atenção Básica em Sergipe. Trata-se de um estudo transversal com caráter quali-quantitativo, com abordagens descritiva e analítica. A amostra foi de 13 fisioterapeutas com predomínio de mulheres, brancas, nascidas em capitais de estados e com 12 ou mais anos de formadas. Com relação às ferramentas tecnológicas do Núcleo Ampliado de Saúde da Família, o apoio matricial foi a única utilizada por todos os participantes. As visitas domiciliares (61,5%) foram as atividades mais realizadas, sendo que dessas, 84,6% ocorreram em equipe e para a população idosa. As ações semanais tinham ênfase na promoção da saúde e prevenção de doenças (69,2%). Quanto ao processo de trabalho, 76,92% relataram que não havia avaliação conjunta entre o Núcleo e gestores, mesma frequência dos que relataram não haver interação entre o Núcleo e o Conselho Municipal de Saúde. Todos afirmaram que ocorria articulação entre o Núcleo e a Equipe de Saúde Família, sendo reconhecido por 53,85% que essa articulação era estabelecida de forma satisfatória. Também foi observada deficiência nos conhecimentos a respeito do Núcleo Ampliado de Saúde da Família e a carência na oferta de capacitações.

The aim is to analyze the socioeconomic characteristics, qualification and the performance of physical therapists in the Family Health and Primary Care Extended Centers in Sergipe. This is a cross-sectional study with a qualitative and quantitative character, with descriptive and analytical approaches. The sample consisted of 13 physiotherapists in which predominantly white women, born in capital and aged 12 or more years since graduation. Regarding the Family Health and Primary Care Extended Centers technological tools, matrix support was the only one used by all participants. Home visits (61.5%) were the most performed activities, of which 84.6% occurred in teams for the elderly population. The weekly actions had an emphasis on health promotion and disease prevention (69.2%). In the work process, 76.9% reported that there was no joint assessment between Family Health and Primary Care Extended Center and manager. The same frequency between Family Health Team and Primary Care Extended Center and municipal health council. Between the Family Health and Primary Care Extended Center and the Family Health Team, all physical therapists reported occurring. There was also a lack of knowledge about the Family Health and Primary Care Extended Center and a lack of training provision.

Multiple introductions and country-wide spread of DENV-2 genotype II (Cosmopolitan) in Brazil.

Dengue virus serotype 2, genotype Cosmopolitan (DENV-2-GII), is one of the most widespread DENV strains globally. In the USA, DENV-2 epidemics have been dominated by DENV-2 genotype Asian-American (DENV-2-GIII), and the first cases of DENV-2-GII were only described in 2019, in Peru, and in 2021 in Brazil. To gain new information about the circulation of DENV-2-GII in Brazil, we sequenced 237 DENV-2 confirmed cases sampled between March 2021 and March 2023 and revealed that DENV-2-GII is already present in all geographic regions of Brazil. The phylogeographic analysis inferred that DENV-2-GII was introduced at least four times in Brazil, between May 2020 and August 2022, generating multiple clades that spread throughout the country with different success. Despite multiple introductions of DENV-2-GII, analysis of the country-wide laboratory surveillance data showed that the Brazilian dengue epidemic in 2022 was dominated by DENV-1 in most states. We hypothesize that massive circulation of DENV-2-GIII in previous years in Brazil might have created a population immune barrier against symptomatic homotypic reinfections by DENV-2-GII, leading to sustained cryptic circulation in asymptomatic cases and localized outbreaks of this new genotype. In summary, our study stresses the importance of arboviral genomic surveillance to close monitoring and better understanding the potential impact of DENV-2-GII in the coming years.

Linfoma no Hodgkin difuso de células B grande como linfoma primario de ovario. Presentación decaso / Diffuse large B-cell non-Hodgkin's lymphoma as primary ovarian lymphoma. Case presentation

Fundamento: Los linfomas primarios de ovario son poco frecuentes; el 1porciento de estos se presenta en ovario y el 1.5 porciento de los tumores malignos de ovario son linfomas. Los tipos histológicos más frecuenteses el linfoma no Hodgkin difuso de células B grande y el BurKitt; el tratamiento consiste en cirugía combinada con quimioterapia.Objetivo: Reportar un caso de un linfoma no Hodgkin difuso de células B grande primario de ovario. Presentación de caso: Se presentó el caso de una paciente de 39 años de edad, con antecedentes patológicos personales de salud; la cual fue al cuerpo de guardia de ginecología por presentar dolor abdominal difuso que no se aliviaba con analgésicos. En la exploración física presentaba dolor a la palpación superficial y profunda en hipocondrio y fosa ilíaca derecha con masa tumoral palpable. Ecografía hacia proyección anexial derecha se observó una imagen de baja ecogenicidad y en la laparoscopia de urgencia se concluyó como una formación de aspecto tumoral que parecía corresponderse con ovario derecho. Se le realizó una histerectomía con doble anexectomía. El diagnóstico anatomopatológico fue un linfoma no Hodgkin primario de ovario.Conclusiones: La paciente del caso presentado tuvo una clínica oligosintomática y la confirmación de la enfermedad fue a partir de una muestra quirúrgica, lo que expresa que el diagnóstico del linfoma no Hodgkin de células B es difícil y aunque es poco frecuente siempre se debe tener en cuenta en el diagnóstico diferencial de las tumoraciones unilaterales de ovario. [AU]

Views of primary healthcare physicians on occupational health.

Introduction: The incorporation of occupational health action in healthcare units is a major public health challenge. Objectives: This study aims to the perceptions of primary health care (PHC) physicians about occupational health. Methods: This is a qualitative exploratory-descriptive study was conducted in a municipality located in the central region of the state of Rio Grande do Sul, Brazil. Data were collected through individual interviews using a semi-structured questionnaire, whose first part focused on participants' sociodemographic profile and to professional training, and the second one was specific about the researched theme. Research participants were physicians working in PHC. Professionals with an experience of less than 6 months and those absent during the data collection period due to vacation or sickness were excluded. Results: Ten physicians met inclusion and were therefore interviewed. The main results indicated that the interviewed participants agree that work is an important health condition. They also reported difficulties in taking measures to prevent and promote occupational health and lack of training on the theme. Conclusions: In general, physicians' perception about the theme is based on the implementation of measures mostly targeted at treating users' symptoms, rarely addressing aspects related to prevention or rehabilitation in daily practices. Training about occupational health is insufficient.

Comparación de vía mediastinal posterior versus vía retroesternal para el reemplazo de esófago con ascenso gástrico parcial. Motivo de un cambio de estrategia / Comparison of posterior mediastinal versus retrosternal route for esophageal replacement with partial gastric pull-up. Rationale for a change in strategy

Introducción: existen dos rutas para realizar el reemplazo de esófago (RE), la retroesternal (RRE) y la mediastinal posterior (RMP). El objetivo del estudio es comparar los pacientes que recibieron un ascenso gástrico parcial empleando estas dos rutas. Material y métodos: Se revisaron las historias clínicas de 51 pacientes con ascenso gástrico parcial, en 27 años en el Hospital Garrahan. Se utilizó la vía RRE en 25 casos y la RMP en 26. Fueron comparados los datos epidemiológicos de los grupos y las variables para valorar la dificultad del acto quirúrgico, evolución inmediata y alejada. El estudio es comparativo, retro-prospectivo y longitudinal. Resultados: las características generales de los pacientes fueron similares. Los que recibieron el ascenso gástrico por vía RMP presentaron una menor incidencia de dehiscencia (p=0,017), de enfermedad por reflujo gastroesofágico (ERGE) (p=0,001) y de dumping (p=0,0001). No hubo diferencias estadísticamente significativas entre los dos grupos al comparar la duración del procedimiento, días de internación total y en Unidad de Cuidados Intensivos (UCI), días de permanencia en asistencia respiratoria mecánica (ARM), inicio de alimentación oral y estenosis de la anastomosis. Se observó una tendencia clínicamente relevante, que no alcanzó significancia estadística en las complicaciones intraquirúrgicas y número de dilataciones postoperatorias. No hubo necrosis del ascenso. Fallecieron 2 pacientes. Conclusiones: considerando la menor incidencia de dehiscencia, ERGE y dumping reemplazados por RMP, elegimos a ésta como nuestra primera opción para el reemplazo esofágico en la infancia (AU)

Introduction: The two routes for esophageal replacement (ER) are retrosternal (RRE) and posterior mediastinal (PMR). The aim of the study was to compare patients who received a partial gastric pull-up using either of these two routes. Material and methods: The clinical records of 51 patients who underwent partial gastric pull-up over 27 years at the Garrahan Hospital were reviewed. The RRE route was used in 25 and the RMP in 26 cases. The epidemiological data of the groups and the variables to evaluate the complexity of the surgical procedure, and shortand long-term outcome were compared. A comparative, retroprospective, and longitudinal study was conducted. Results: the general characteristics of the patients were similar. Those who underwent gastric pull-up via PMR had a lower incidence of dehiscence (p=0.017), gastroesophageal reflux disease (GERD) (p=0.001), and dumping (p=0.0001). No statistically significant differences were found between the two groups when comparing the duration of the procedure, days of total hospital and intensive care unit (ICU) stay, days on mechanical ventilation (MV), initiation of oral feeding and stenosis of the anastomosis. A clinically relevant trend, which did not reach statistical significance, was observed in intraoperative complications and number of postoperative dilatations. There was no necrosis of the pull-up. Two patients died. Conclusions: considering the lower incidence of dehiscence, GERD, and dumping associated with PMR, this was our first choice for esophageal replacement in infancy (AU)

3D Printing of a Graphene-Modified Photopolymer Using Stereolithography for Biomedical Applications: A Study of the Polymerization Reaction.

Additive manufacturing is gaining importance thanks to its multiple advantages. Stereolithography (SLA) shows the highest accuracy and the lowest anisotropy, which has facilitated the emergence of new applications as dentistry or tissue engineering. However, the availability of commercial photopolymers is still limited, and there is an increasing interest in developing resins with properties adapted for these new applications. The addition of graphene-based nanomaterials (GBN) may provide interesting advantages, such as improved mechanical properties and bioactivity. However, there is a lack of knowledge regarding the effect of GBNs on the polymerization reaction. A photopolymerizable acrylic resin has been used, and the effect of the addition of 0.1wt% of graphene (G); graphene oxide (GO) and graphite nanoplatelets (GoxNP) on printability and polymerization have been investigated. It was observed that the effect depended on GBN type, functionalization and structure (e.g., number of layers, size, and morphology) due to differences in the extent of dispersion and light absorbance. The obtained results showed that GO and GoxNP did not significantly affect the printability and quality of the final structure, whilst the application of G exhibited a negative effect in terms of printability due to a reduction in the polymerization degree. GO and GoxNP-loaded resins showed a great potential to be used for manufacturing structures by SLA.

Zika-Associated Birth Defects Reported in Pregnancies with Laboratory Evidence of Confirmed or Possible Zika Virus Infection - U.S. Zika Pregnancy and Infant Registry, December 1, 2015-March 31, 2018.

Zika virus infection during pregnancy can cause serious birth defects of the brain and eyes, including intracranial calcifications, cerebral or cortical atrophy, chorioretinal abnormalities, and optic nerve abnormalities (1,2). The frequency of these Zika-associated brain and eye defects, based on data from the U.S. Zika Pregnancy and Infant Registry (USZPIR), has been previously reported in aggregate (3,4). This report describes the frequency of individual Zika-associated brain and eye defects among infants from pregnancies with laboratory evidence of confirmed or possible Zika virus infection. Among 6,799 live-born infants in USZPIR born during December 1, 2015-March 31, 2018, 4.6% had any Zika-associated birth defect; in a subgroup of pregnancies with a positive nucleic acid amplification test (NAAT) for Zika virus infection, the percentage was 6.1% of live-born infants. The brain and eye defects most frequently reported included microcephaly, corpus callosum abnormalities, intracranial calcification, abnormal cortical gyral patterns, ventriculomegaly, cerebral or cortical atrophy, chorioretinal abnormalities, and optic nerve abnormalities. Among infants with any Zika-associated birth defect, one third had more than one defect reported. Certain brain and eye defects in an infant might prompt suspicion of prenatal Zika virus infection. These findings can help target surveillance efforts to the most common brain and eye defects associated with Zika virus infection during pregnancy should a Zika virus outbreak reemerge, and might provide a signal to the reemergence of Zika virus, particularly in geographic regions without ongoing comprehensive Zika virus surveillance.

Priming with a gonadotropin-releasing hormone agonist before immature oocyte retrieval may improve maturity of oocytes and outcome in in vitro maturation (IVM) cycle: a case report.

BACKGROUND: The concept of using a gonadotropin-releasing hormone agonist (GnRH-a) instead of human chorionic gonadotropin for triggering ovulation in patients treated with an antagonist protocol for in vitro fertilization (IVF) has become a routine clinical practice. It may promote oocyte nuclear maturation, resumption of meiosis and cumulus expansion. It seems that this attempt could be beneficial in an in vitro maturation (IVM) oocyte cycle performed for polycystic ovarian syndrome as well as for other indications such as urgent fertility preservation in patients with malignancies or unusual indications. CASE PRESENTATION: We present the case of a Caucasian patient who needed fertility preservation when routine natural IVF treatment did not yield oocyte retrieval, followed by three IVM cycles, priming ovulation with a GnRH-a. In total, 12 oocytes were obtained, all matured 4.5 hours after incubation in maturation media. The fertilization rate after intracytoplasmic sperm injection was 83%. Six good-quality embryos were vitrified. CONCLUSIONS: It seems that triggering with a GnRH-a in selected cases may replace human chorionic gonadotropin in IVM of oocytes and could be highly beneficial in terms of obtaining high-grade embryos and possible pregnancy.

Development and implementation of a brief chat-based intervention to support mental health during the COVID-19 pandemic / Desenvolvimento e implementação de uma intervenção breve via chat para suporte em saúde mental durante a pandemia da COVID-19 / Desarrollo e implementación de una intervención breve vía chat para dar apoyo en salud mental durante la pandemia de COVID-19

This paper presents the development and implementation of a brief chat-based intervention for mental health support toward people suffering from the context of the COVID-19 pandemic in Brazil and Argentina. During the development phase, we proposed a protocol that comprised: 1) screening and needs assessment; 2) health education based on active listening techniques; and 3) referral to available materials and crisis services available (e.g., crisis intervention hotlines). In the implementation phase, we recruited and trained 77 volunteers who provide healthcare chat support for users under the supervision of 20 psychologists. In less than two months, we performed 1.107 sessions. We expect that the healthcare chat support might be a valuable resource during the COVID-19 pandemic, although further studies to assess its feasibility and effectiveness are needed.

Este artigo apresenta o desenvolvimento e a implementação de uma intervenção breve via chat para suporte em saúde mental voltada para pessoas em sofrimento decorrente do contexto da pandemia de COVID-19, no Brasil e na Argentina. Durante a fase de desenvolvimento, foi proposto um protocolo que inclui: 1) triagem e avaliação de necessidades; 2) educação em saúde com base em técnicas de escuta ativa e 3) encaminhamento de materiais e contatos de serviços especializados ou de urgência disponíveis (ex. linhas diretas de intervenção em crise). Na fase de implementação, foram recrutados e treinados 77 voluntários que oferecem acolhimento em saúde via chat aos usuários sob a supervisão de 20 psicólogos. Em menos de 2 meses, 1.107 intervenções foram realizadas. Espera-se que a intervenção via chat possa ser um recurso valioso durante a pandemia de COVID-19, embora estudos adicionais sejam necessários para avaliar sua viabilidade e sua efetividade.

Este artículo presenta el desarrollo e implementación de una intervención breve vía chat para dar apoyo en salud mental destinado a personas que presentan sufrimientos debido al contexto de la pandemia de COVID-19, en Brasil y Argentina. Durante la fase del desarrollo fue propuesto un protocolo que incluye: 1) detección y evaluación de necesidades; 2) educación para la salud basada en técnicas de escucha activa y 3) derivación de materiales y contactos de servicios especializados o de emergencia disponibles (ej. líneas directas de intervención en crisis). En la fase de implementación, fueron reclutados y capacitados 77 voluntarios que ofrecen contención en salud vía chat a los usuarios bajo la supervisión de 20 psicólogos. En menos de 2 meses se realizaron 1.107 intervenciones. Se espera que la intervención vía chat pueda ser un recurso valioso durante la pandemia de COVID-19, aunque se necesitan estudios adicionales para evaluar su viabilidad y efectividad.

Methyl Jasmonate Applications From Flowering to Ripe Fruit Stages of Strawberry (Fragaria × ananassa 'Camarosa') Reinforce the Fruit Antioxidant Response at Post-harvest.

Preharvest applications of methyl jasmonate (MeJA) have been shown to improve post-harvest fruit quality in strawberry fruit. However, the effectiveness of consecutive field applications at different phenological stages on the reinforcement of the antioxidant capacity remains to be analyzed. To determine the best antioxidant response of strawberry (Fragaria × ananassa 'Camarosa') fruit to different numbers and timing of MeJA applications, we performed three differential preharvest treatments (M1, M2, and M3) consisted of successive field applications of 250 µmol L-1 MeJA at flowering (M3), large green (M2 and M3), and ripe fruit stages (M1, M2, and M3). Then, we analyzed their effects on fruit quality parameters [firmness, skin color, soluble solids content/titratable acidity (SSC/TA) ratio, fruit weight at harvest, and weight loss] along with anthocyanin and proanthocyanidin (PA) accumulation; the antioxidant-related enzymatic activity of catalase (CAT), guaiacol peroxidase (POX), and ascorbate peroxidase (APX); the total flavonoid and phenolic contents, antioxidant capacity, and ascorbic acid content (AAC) during post-harvest storage (0, 24, 48, and 72 h). We also evaluated the effect on lignin, total carbon and nitrogen (%C and N), lipid peroxidation, and C and N isotopes signatures on fruits. Remarkably, the results indicated that MeJA treatment increases anthocyanin and PA contents as well as CAT activity in post-harvest storage, depending on the number of preharvest MeJA applications. Also, M3 fruit showed a higher AAC compared to control at 48 and 72 h. Noticeably, the anthocyanin content and CAT activity were more elevated in M3 treatment comparing with control at all post-harvest times. In turn, APX activity was found higher on all MeJA-treated fruit independent of the number of applications. Unlike, MeJA applications did not generate variations on fruit firmness and weight, lignin contents,% C and N, and in lipid peroxidation and water/nitrogen use efficiency according to C and N isotope discrimination. Finally, we concluded that an increasing number of MeJA applications (M3 treatment) improve anthocyanin, PA, AAC, and CAT activity that could play an essential role against reactive oxygen species, which cause stress that affects fruits during post-harvest storage.

Marjolin's Ulcer in Laron Syndrome - an Unexpected Combination: A Case Report.

Marjolin's ulcer is an atypical malignancy that develops from deep scars of chronically traumatised skin. Laron syndrome (LS) is a rare autosomal recessive growth retardation from a mutation in the growth hormone receptor (GHR) gene leading to defective GHR, growth hormone insensitivity and eventual low levels of insulin-like growth factor type 1 (IGF-1). Affected individuals present with proportionate dwarfism and other characteristic physical defects, but at the same time are conferred protection against cancer due to low serum levels of IGF-1. We report an exceptional case of Marjolin's ulcer in the foot of a female LS patient 30 years after she sustained flame burns as a 6-month-old baby. Three months before coming to us, she had a 2x3cm ulcer that turned into a rapidly enlarging fungating mass involving the leg, ankle, and foot. Histopathologic analysis of an incision biopsy showed well-differentiated squamous cell carcinoma. The extent of her lesion precluded wide excision. Below knee amputation was done. A second biopsy confirmed the histopathologic diagnosis. This is the first reported case in the literature of Marjolin's ulcer in LS which raises the possibility that IGF-1 deficiency does not completely protect against squamous cell cancer.

Priming of Defense Systems and Upregulation of MYC2 and JAZ1 Genes after Botrytis cinerea Inoculation in Methyl Jasmonate-Treated Strawberry Fruits.

Several attempts have been made to study the effects of methyl jasmonate (MeJA) on plants in the past years. However, the comparative effects of the number and phenological time of MeJA applications on the activation of defense systems is currently unknown in strawberries. In the present research, we performed three field treatments during strawberry (Fragaria× ananassa 'Camarosa') fruit development and ripening which consisted of differential MeJA applications at flowering (M3), and the large green (M2 and M3) and red ripe (M1, M2, and M3) fruit stages. We also checked changes in gene expression related to plant defense against Botrytis cinerea inoculation post-harvest. In M3 treatment, we observed an upregulation of the anthocyanin and lignin contents and the defense-related genes, encoding for chitinases, ß-1,3-glucanases and polygalacturonase-inhibiting proteins, after harvest (0 hpi), along with the jasmonate signaling-related genes FaMYC2 and FaJAZ1 at 48 h after B. cinerea inoculation (48 hpi) during postharvest storage. Although we did not find differences in gray mold incidence between the MeJA treatments and control, these results suggest that preharvest MeJA treatment from the flowering stage onwards (M3) primes defense responses mediated by the upregulation of different defense-related genes and retains the upregulation of MYC2 and JAZ1 at 48 hpi.

Sentiment Analysis in Children with Neurodevelopmental Disorders in an Ingroup/Outgroup Setting.

People punish transgressors with different intensity depending if they are members of their group or not. We explore this in a cross-sectional analytical study with paired samples in children with developmental disorders who watched two videos and expressed their opinion. In Video-1, a football-player from the participant's country scores a goal with his hand. In Video-2, a player from another country does the same against the country of the participant. Each subject watched the two videos and their answers were compared. The autism spectrum disorder (ASD) group showed negative feelings in Video 1 (M = - .1; CI 95% - .51 to .31); and in Video 2 (M = - .43; CI 95% .77 to - .09; t(8) = 1.64, p = .13), but the attention deficit hyperactivity disorder, learning disabilities, intellectual disability groups showed positive opinion in Video-1 and negative in Video-2. This suggests that children with ASD respect rules regardless of whether those who break them belong or not to their own group, possibly due to lower degrees of empathy.

Graphene Oxide and Graphene Reinforced PMMA Bone Cements: Evaluation of Thermal Properties and Biocompatibility.

The incorporation of well-dispersed graphene oxide (GO) and graphene (G) has been demonstrated as a promising solution to improve the mechanical performance of polymethyl methacrylate (PMMA) bone cements in an attempt to enhance the long-term survival of the cemented orthopaedic implants. However, to move forward with the clinical application of graphene-based PMMA bone cements, it is necessary to ensure the incorporation of graphene-based powders do not negatively affect other fundamental properties (e.g., thermal properties and biocompatibility), which may compromise the clinical success of the implant. In this study, the effect of incorporating GO and G on thermal properties, biocompatibility, and antimicrobial activity of PMMA bone cement was investigated. Differential scanning calorimetry studies demonstrated that the extent of the polymerisation reaction, heat generation, thermal conductivity, or glass transition temperature were not significantly (p > 0.05) affected by the addition of the GO or G powders. The cell viability showed no significant difference (p > 0.05) in viability when MC3-T3 cells were exposed to the surface of G- or GO-PMMA bone cements in comparison to the control. In conclusion, this study demonstrated the incorporation of GO or G powder did not significantly influence the thermal properties or biocompatibility of PMMA bone cements, potentially allowing its clinical progression.

Graphene and graphene oxide functionalisation with silanes for advanced dispersion and reinforcement of PMMA-based bone cements.

The reinforcement of PMMA bone cements using carbon based nanomaterials has demonstrated to be a potential solution to their poor mechanical properties. The achievement of an optimal dispersion of the nanoparticles within the polymeric matrix is a crucial but not easy stage in the production of high-quality reinforced materials. In this work, a useful route for the graphene (G) functionalisation, via silanisation with (3-methacryloxypropyl) trimethoxy silane (MPS), has been developed, providing a remarkable enhancement in dispersibility and mechanical properties. With the purpose to define the critical graphene surface oxidation parameters for an optimal silanisation, different routes were thoroughly analysed using infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The results showed that the silanisation significantly improved the G dispersibility: whereas the pristine G dispersion fell down within the first 24 h, the silanised G showed an adequate stability after 5 days. Additionally, this improved dispersibility produced a notable increase in the mechanical properties of the G-reinforced bone cements: in comparison with the pristine G, the compression and bending strength of silanised G increased by 12% and by 13.7% respectively and the fracture toughness by 28%. These results provide very useful information on the relevance that the characteristics of the superficial oxidation of graphene have on the effectiveness of the silanisation process, besides an interesting functionalisation procedure for advanced dispersion and reinforcement of G-PMMA bone cements.