Microbiological studies of the round goby (Neogobius melanostomus) from the Puck Bay, southern Baltic Sea.

In summer 2017 numerous dead round gobies ( Neogobius melanostomus) and individuals covered with white coating were observed in the Puck Bay. The aim of our research was to determine the microbiological composition of the round goby from the Puck Bay, focusing on the presence of pathogens. Bacteria were identified by biochemical methods and, by sequencing of 16S rRNA. The dominant bacterial species were Shewanella baltica, Pseudomonas spp. and Aeromonas spp. - opportunistic pathogens, commonly present in many fish species, which may become harmful for the organism in unfavorable conditions. It was the first trial to determine the composition of the bacterial flora of N. melanostomus from that area.

Phenotypical and genotypical characterization of Shewanella putrefaciens strains isolated from diseased freshwater fish.

Between 2007 and 2012, a variety of disease outbreaks most often characterized by skin disorders were observed among different species of freshwater fish in Poland. In most cases, the clinical signs included focally necrotized gills, necrotic skin lesions or ulcers. Internally, haemorrhages, oedematous kidney and abnormal spleen enlargement were generally noted. The disorders were accompanied by increased mortality. Most of the problems concerned cultured common carp Cyprinus carpio L. and rainbow trout Oncorhynchus mykiss (Walbaum). Fish have been examined from a number of these farms, and additionally, the wild and ornamental fish with similar clinical signs of diseases were also tested. Bacteria were isolated consistently from lesions and internal organs. They had characteristic orange-pigmented colonies which grew in pure culture or constituted 55-95% of total bacterial flora. One hundred and eighteen isolates were collected and biochemically identified as Shewanella putrefaciens group, and this was confirmed by sequencing. Challenge tests confirmed the pathogenicity of these bacteria. This is the first report characterizing and describing S. putrefaciens as a pathogen of different species of freshwater fish in Europe.

[A case of empyema after pneumonectomy caused by methicillin-resistant staphylococcus aureus infection treated successfully with local administration with vancomycin]. / Przypadek ropniaka po pneumonektomii wywolanego metycylinoopornym gronkowcem zlocistym wyleczonego stosowana miejscowo wankomycyna.

A 55-year-old man underwent left pneumonectomy due to squamous cell carcinoma. Three weeks later bronchopleural fistula and pleural empyema with MRSA infection were recognized. Treatment was based on closed pleural drainage and antibiotic therapy. Initially patient was treated with trimethoprin-sulfamethoxazole and then vancomycin intravenously but empyema was not cured completely. Therefore repeated instillation of vancomycin into the empyema cavity was applied. After 6 days of treatment, culture studies of the pleural fluid became negative and drainage tube was removed 5 days later. We suggest that local administration of vancomycin is an effective method in postpneumonectomy empyema with MRSA infection.