[The effect of the antioxidant, butylhydroxyanisole on experimental myocardial necrosis in rats. Morphologic study]. / Vplyv antioxidacnej látky butylhydroxyanizolu na experimentálnu nekrózu myokardu potkanov. Morfologické sledovanie.

The present paper aimed to investigate a possible effect of the antioxidant butylhydroxyanisole (BHA) on the extent of experimental necroses of the myocardial cells. Administration of BHA in a daily dose of 10 mg.kg-1 i. m. lasted 20 days. The isoprenaline model in a single-dose subcutaneous administration of IP 25 mg.kg-1 was used to induce experimental necrosis. Tables 1-7 show quantification and morphometry of histological and electron-microscopic images. The protective effect of BHA was manifested by influencing the extent of myocardial necroses (p < 0.01) (Table 1). In the morphometric electron-microscopic examinations, the protective influence of BHA was recorded in the parameter of cristolysis, i.e. equipment of mitochondria with cristae (Table 5), in comparison of the control group with IP and the BHA group with IP (p < 0.01). No statistically significant differences in the two above-mentioned experimental groups were found in the other compartments.

[The effect of butylhydroxyanisole, an antioxidant, on oxidative phosphorylation of myocardial mitochondria in experimental ischemia]. / Vliv antioxidacní látky butylhydroxyanizolu na oxidacní fosforylaci mitochondrií myokardu pri experimentálne vyvolané ischemii.

The present paper evaluated the effect of the antioxidatively acting agent butylhydroxyanisole (BHA) on the oxidative and energetic processes in the mitochondria of the myocardium, both normal and damaged with isoprenaline. BHA was administered for 20 days in a daily dose of 10 mg.kg-1. Experimental necrosis of the myocardium was induced by a single-dose administration of a dose of 25 mg.kg-1 s.c. The results shown in Graphs 1-7 indicate that BHA in the above-mentioned concentration did not act protectively on the metabolic processes taking place in the mitochondria during experimentally induced necroses of the myocardium.

[The effect of butylhydroxyanisole, an antioxidant, on experimental atherosclerosis in Japanese quail]. / Vplyv antioxidantu butylhydroxyanizolu na experimentálnu aterosklerózu u japonských prepelíc.

The present paper aimed to examine a possible influence of the antioxidant butylhydroxyanisole (BHA) on the extent of atherosclerotic changes in coronary arterioles, in arcus aortae, and on the influencing of the extent of myocardial necroses. The model of atherosclerosis was worked out by administering 1% cholesterol diet to Japanese quails for the period of 40 days. The above-mentioned changes were examined on histological preparations and they were evaluated morphometrically. Quantification and morphometry of atherosclerotic changes in the individual experimental groups are shown in Table 1 and statistical evaluation of findings using the t-test in Tables 2, 3 and 4. The protective effect of butylhydroxyanisole was demonstrated to be statistically significant in the examination of the extent of atheromatous changes in arterioles. In arcus aortae, the findings were neither extensive, nor statistically significant. The findings concerning the extent of necroses in the myocardium provide documentary evidence for subendothelial micronecroses in the group with BHA in contrast to blended necroses in the group with Epavit + cholesterol. The model of cholesterol diet used in Japanese quails proved to be suitable to examine the pathogenesis of atherosclerosis and its possible influencing by pharmaceuticals. The dose of butylhydroxyanisole (BHA), 20 mg.kg-1, was selected in the optimal manner with regard to possible inhibition of atherosclerotic changes.

Biochemical, structural and ultrastructural changes in the Prague hereditary hypercholesterolemic (PHHC) rats heart after the long treatment with calcium channel blockers.

Elevated plasma cholesterol concentration may be important in the initiation of heart ischemia and progression of atherogenesis. It has been shown that drugs affecting calcium entry into cells can attenuate the development of this cholesterol induced changes. The aim of this work was to study the influence of five calcium channel blockers 6 weeks treatment on some parameters of lipid metabolism, morphological and ultrastructural changes in the Prague hereditary hypercholesterolemic (PHHC) rats myocardium. The calcium antagonists were administered twice daily perorally in the following doses: nifedipine and nitrendipine 0.2 mg.kg-1, nimodipine 2.5 mg.kg-1, verapamil and diltiazem 1.0 mg.kg-1 body weight. Cholesterol diet decreased the level of free fatty acids significantly (from 5.59 +/- 0.216 to 3.83 +/- 0.371 mumol.g-1), increased the level of total cholesterol (from 28.0 +/- 1.92 to 34.0 +/- 2.90 mumol.g-1) and caused micronecrosis. Examination of myocardial ultrastructure showed a frequent occurrence of lysosomes as well as large numerous autophagic vacuoles and contracture bands of myofibrils. These effects were partly suppressed by calcium channel blockers verapamil and diltiazem, but dihydropyridines were not effective. Observed biochemical changes were in accordance with structural and ultrastructural investigations.

[The effect of verapamil on experimental atherosclerosis in rabbits]. / Vplyv verapamilu na experimentálnu aterosklerózu u králikov.

The model of atherosclerosis was elaborated by administration of cholesterol to rabbits in daily doses of 70 g per animal over a period of 3 months. The model served to study the effect of the calcium entry blocker Verapamil as to its potential capacity of affecting the extent of atherosclerotic changes. The thoracic aorta, the abdominal aorta, the right coronary artery and the apex of the myocardium were removed for histological evaluation. Atherosclerotic changes were assessed by light microscopy using the method of quantifying the thickening of the intima of vessels. The extent of atherosclerotic changes in the coronary arteries and arterioles was evaluated morphometrically. Further the presence and extent of necroses of the myocardium as well as endothelial proliferation in the capillaries of the cardiac muscle were recorded. Evaluation of the obtained results calculated as percentage of the presence of findings demonstrated a significant protective effect of Verapamil on the parameters studied.