[Immunoenzyme analysis of placenta-specific alpha 1-microglobulin in the serum of blood donors]. / Immunofermentnyi analiz platsenta-spetsificheskogo alpha 1-mikroglobulina v syvorotke krovi donorov.

An immunoenzyme assay is developed for quantitative estimation of placenta specific alpha 1-microglobulin in biological fluids. The assay is more sensitive as compared with immunodiffusion and radioimmunoassays used previously. Concentration of alpha 1-microglobulin in blood serum of men-donors was found to be 7-30 ng/ml and of women--0.50 ng/ml.

[Physico-chemical study of estrogen-binding alpha-globulin in humans]. / Fiziko-khimicheskoe izuchenie éstrogensviazyvaiushchego alpha-globulina cheloveka.

Human estrogen-binding alpha-globulin (EBG) was shown to be a thermolabile glycoprotein with molecular mass of 290,000 daltons, its isoelectric point occurred at pI 6.02-6.04. The globulin was salted out with 50-70% of ammonium sulfate. Some organic and inorganic acids precipitated EBG. DNAase and RNAase did not affect the glycoprotein but trypsin hydrolyzed its molecule. The EBG preparation obtained may be used for production of monospecific antiserum in order to study the glycoprotein functions in a body.

[Immunochemical study of estrogen-binding alpha-globulin in the tissues and blood serum of cancer patients]. / Immunokhimicheskoe izuchenie éstrogensviazyvaiushchego al'fa-globulina v tkaniakh i syvorotke krovi onkologicheskikh bol'nykh.

EBA distribution in tumour and normal tissues was investigated using immunochemical method. The antigen was found in spleen, kidney and mammary gland extracts, as well as in the extracts of the malignant stomach tissue, throat, kidney, urinary bladder, testicles, i.e. this antigen can be secreted into blood in some forms of malignant neoplasia and is, consequently, a tumour-associated antigen. Further investigations will show, if this protein can be used for the diagnosis of malignant diseases.

[Comparative immunochemical and physicochemical study of 2 alpha-glycoproteins from human kidney tumors]. / Sravnitel'noe immunokhimicheskoe i fiziko-khimicheskoe izuchenie dvukh al'fa-glikoproteidov opukholei pochki cheloveka.

Using immunochemical methods two "divergent" antigens were identified in tumors of kidney; these antigens were not found in normal kidney, but were observed in several other normal tissues. Both antigens were alpha-glycoproteins. Immunochemical analysis was carried out to find these proteins in extracts of normal tissues, in malignant tumors of kidney as well as in blood serum of patients with the kidney tumors. These proteins were-dissimilar in immunochemical properties and also in some physico-chemical characteristics; one of them was a thermostable protein and the other one was able to bind estrogens.

[Immunochemical study of the alpha-globulins in human kidney tumor tissue]. / Immunokhimicheskoe izuchenie al'fa-globulinov v opukholevoi tkani pochki cheloveka.

Four individual tissue proteins were studied both in normal and tumoral tissue of kidney (hypernephroma, Wilms tumor) using immunochemical analysis. Occurrence of an antigen, which was absent in definitive tissue of homologous origin, as well as a decrease in biosynthesis of some homologous antigens were found in malignant tissue.