ABSTRACT
In this investigation the utility of evaporative light scattering detection (ELSD) combined with HPLC-MS was demonstrated as a key component of a bioassay-guided fractionation, or "biofractionation" technique, for the evaluation of high throughput screen actives. ELSD provided on-line analyte mass information that was critical for the classification of the samples. Chemiluminescent nitrogen detection (CLND) was also evaluated for sample concentration estimation for nitrogen-containing compounds, and accurate mass LC-MS-MS analysis was employed for rapid structural confirmation and elucidation of components previously identified as active via biofractionation.
Subject(s)
Chromatography, High Pressure Liquid/methods , Mass Spectrometry/methods , Nitrogen/analysis , Biological Assay , Light , Luminescent Measurements , Scattering, RadiationABSTRACT
The products of aqueous chlorination reactions of 1-methylnaphthalene, fluorene, dibenzofuran, anthracene, phenanthrene, 1-methylphenanthrene, fluoranthene, and pyrene have been determined. The conditions employed for these reactions approximated those that might be encountered in water treatment facilities. Reactions at pH greater than 6 tended to produce oxygenated products (epoxides, phenols, quinones, etc.), and reactions at pH less than 6 tended to produce both oxygenated (quinones) and chlorinated products.