ABSTRACT
We developed a technique using nonlinear correlation of photoluminescence (PL) to characterize midwave infrared lasers by extracting the density and temperature dependence of the carrier lifetime and its exact branching into radiative and nonradiative processes. This was accomplished, without time resolving the PL recovery, through mathematical optimization. We extracted this information by using a laser source that can be operated in both continuous-wave and short pulse modes. Through fitting of the PL signal and its nonlinear correlation for both laser modes of operation, the carrier lifetime as a function of density is extracted. As a proof of principle, we investigated a midinfrared Sb based laser and showed that the radiative branching ratio drops from approximately 54% at 80 K to about 3% at room temperature, resulting from an order of magnitude increase in the nonradiative rate coupled with a factor of 2 reduction in the radiative rate. We believe that this is a very generic approach and can be extended to various luminescing material systems.
ABSTRACT
Rheumatoid arthritis and periodontitis are inflammatory diseases modulated by proinflammatory cytokines [e.g. interleukin (IL-1) 1 and tumour necrosis factor alpha], which activate local fibroblasts to do the following: (1) proliferate, (2) induce gene expression and (3) produce destructive metalloproteinases. Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor (composed of HIF-1alpha and HIF-1beta/aryl hydrocarbon receptor nuclear transporter) that is modulated by hypoxia. HIF-1 binds to and induces several genes containing an HIF-1 consensus-binding site, including vascular endothelial growth factor and several glycolytic enzymes. Through differential screening of a human synovial fibroblast cDNA library, we identified HIF-1alpha as a clone up-regulated by IL-1. The mRNA for HIF-1alpha subunit was increased 3-4-fold by Northern blot analysis after cells had been incubated for 3 h in the presence of IL-1. In addition, IL-1 increased the binding of the heterodimer HIF-1 to the HIF consensus sequence. These results suggest that HIF-1 might have a role in inflammation, possibly in attempting to re-establish homoeostasis.
Subject(s)
DNA-Binding Proteins/biosynthesis , Gingiva/metabolism , Interleukin-1/physiology , Nuclear Proteins/biosynthesis , Synovial Membrane/metabolism , Transcription Factors , Base Sequence , Cells, Cultured , DNA Primers , DNA, Complementary , DNA-Binding Proteins/genetics , Gingiva/cytology , Humans , Hypoxia-Inducible Factor 1 , Hypoxia-Inducible Factor 1, alpha Subunit , Lipopolysaccharides/pharmacology , Nuclear Proteins/genetics , RNA, Messenger/genetics , Synovial Membrane/cytology , Tumor Necrosis Factor-alpha/pharmacologyABSTRACT
High levels of interleukin 1 (IL-1) found in inflammatory diseases such as rheumatoid arthritis and periodontitis act on the local fibroblasts, resulting in an altered phenotype characterized by hyperplasia and the production of inflammatory mediators and destructive enzymes. The goal of this study was to identify genes induced as an early response to IL-1 in synovial and gingival fibroblasts which might play a regulatory role in the cascade of events leading to their activation. Using the technique of mRNA differential display, we have identified the mitogen-inducible nuclear orphan receptor (MINOR) as a gene up-regulated by IL-1 in human synovial and gingival fibroblasts. The rapid induction of both mRNA and DNA binding activity suggests that MINOR may play an important early role in regulating the response of fibroblasts to inflammation.
Subject(s)
Gingiva/drug effects , Interleukin-1/pharmacology , Receptors, Cytoplasmic and Nuclear/biosynthesis , Synovial Fluid/drug effects , Transcription Factors/biosynthesis , Blotting, Northern , Cells, Cultured , DNA-Binding Proteins , Fibroblasts/cytology , Fibroblasts/drug effects , Fibroblasts/metabolism , Gingiva/cytology , Gingiva/metabolism , Humans , Nerve Tissue Proteins , RNA/analysis , RNA, Messenger/drug effects , Receptors, Steroid , Receptors, Thyroid Hormone , Synovial Fluid/cytology , Synovial Fluid/metabolismABSTRACT
The organization, financing, and delivery of publicly funded behavioral health services are undergoing massive changes nationwide. Managed care principles and practices are being implemented widely and are being relied on increasingly to meet the challenges of containing costs and improving service effectiveness. To meet these goals, comprehensive systems are under development for measuring and reporting outcomes experienced by individuals who received services and for assessing the impact of managed care strategies on the service delivery system. This article presents an example from the Prepaid Mental Health Program in New York State. It highlights the development, implementation, and early experiences with the plan's performance management system for public sector managed behavioral health, a basis for continuous quality improvement activities and information reporting products such as report cards. Policy, administrative, and financial implications are illuminated.
Subject(s)
Managed Care Programs/organization & administration , Mental Health Services/organization & administration , Prepaid Health Plans/organization & administration , Public Health Administration/standards , Total Quality Management/organization & administration , Humans , Information Services , Managed Care Programs/standards , Mental Health Services/standards , New York , Prepaid Health Plans/standards , Program Development , Program Evaluation , Quality Indicators, Health CareABSTRACT
Rheumatoid arthritis and periodontitis are chronic inflammatory diseases associated with tissue destruction that is mediated in part by elevated levels of cytokines (e.g., interleukin-1 and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library for interleukin-1 induced genes revealed a clone identical to the gene encoding human bone morphogenetic protein-2. Northern blot analysis of human synovial fibroblast mRNA confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1. Utilizing a specific antibody, levels of bone morphogenetic protein-2 protein in conditioned medium from synovial fibroblasts were also up-regulated in the presence of interleukin-1. This is the first report of the production of bone morphogenetic protein-2 by synovial fibroblasts, and the first report of its up-regulation in response to interleukin-1. However, interleukin-1 did not induce bone morphogenetic protein-2 mRNA in human gingival fibroblasts.
Subject(s)
Bone Morphogenetic Proteins/biosynthesis , Gene Expression Regulation/drug effects , Gingiva/metabolism , Interleukin-1/pharmacology , Synovial Membrane/metabolism , Transforming Growth Factor beta , Bone Morphogenetic Protein 2 , Cells, Cultured , Culture Media, Conditioned , Fibroblasts/drug effects , Fibroblasts/metabolism , Gene Library , Humans , Osteoarthritis/metabolism , Protein Biosynthesis/drug effects , RNA, Messenger/biosynthesis , Transcription, Genetic/drug effectsABSTRACT
In Arabidopsis seedlings germinated and grown in continuous light, CAT2 mRNA abundance peaks 1 d after imbibition, consistent with the role of catalase in detoxifying H2O2 generated during the [beta]-oxidation of fatty acids stored in the seed. A second peak of CAT2 mRNA abundance, of lower amplitude than the initial peak, appears 6 d after imbibition and may be associated with the development of photosynthetic competence and induction of photorespiration. This second peak in steady-state CAT2 mRNA abundance is regulated by light and is not seen in etiolated seedlings. CAT2 mRNA accumulation is induced by exposure to high-fluence blue or far-red light but not by red light. In addition, light induction is unaffected by several mutations that block blue light-mediated inhibition of hypocotyl elongation (blu1, blu2, blu3, hy4), suggesting phytochrome involvement. When etiolated seedlings are transferred to continuous white light, CAT2 mRNA rapidly (within 30 min) accumulates. It is interesting that in these seedlings CAT2 mRNA abundance undergoes pronounced oscillations with a circadian (24 h) periodicity, indicating control by the endogenous circadian clock. No such oscillations are detected in CAT2 mRNA abundance in etiolated seedlings prior to illumination. Control of CAT2 expression by the circadian clock is also seen in 5-week-old plants grown in a light-dark cycle and transferred either to continuous dark or to continuous light; in continuous light the circadian oscillations in CAT2 mRNA abundance persist for at least five circadian cycles, indicating the robustness of this circadian rhythm.
ABSTRACT
The lignin peroxidases (LiPs) of white-rot basidiomycetes are generally thought to catalyze the oxidative cleavage of polymeric lignin in vivo. However, direct evidence for such a role has been lacking. In this investigation, 14C- and 13C-labeled synthetic lignins were oxidized with a purified isozyme of Phanerochaete chrysosporium LiP. Gel permeation chromatography of the radiolabeled polymers showed that LiP catalyzed their cleavage to give soluble lower-M(r) products. To a lesser extent, the enzyme also polymerized the lignins to give soluble higher-M(r) products. This result is attributable to the fact that purified LiP, unlike the intact fungus, provides no mechanism for the removal of lignin fragments that are susceptible to repolymerization. LiP catalysis also gave small quantities of insoluble, perhaps polymerized, lignin, but in lower yield than intact P. chrysosporium does. 13C NMR experiments with 13C-labeled polymer showed that LiP cleaved it between C alpha and C beta of the propyl side chain to give benzylic aldehydes at C alpha, in agreement with the cleavage mechanism hypothesized earlier. The data show that LiP catalysis accounts adequately for the initial steps of ligninolysis by P. chrysosporium in vivo.
Subject(s)
Lignin/metabolism , Peroxidases/metabolism , Benzyl Alcohols/metabolism , Carbon Isotopes , Carbon Radioisotopes , Lignin/chemical synthesis , Magnetic Resonance Spectroscopy , Radioisotope Dilution TechniqueABSTRACT
1H NMR spectra at 200- and 600-MHz of manganese peroxidase from Phanerochaete chrysosporium and of its cyanide derivative are reported. The spectrum of the native protein is very similar to that of other peroxidases. The assignment of the spectrum of the cyanide derivative has been performed through 1D NOE, 2D NOESY, and COSY experiments. This protein is very similar to lignin peroxidase, the only meaningful difference being the shift of H delta 2 of the proximal histidine. The spectra of the cyanide derivative of these two proteins are compared with those of horseradish peroxidase and cytochrome c peroxidase. The shift pattern of the protons of the proximal histidine is discussed relative to the structural properties which affect the Fe3+/Fe2+ redox potential.
Subject(s)
Fungi/enzymology , Magnetic Resonance Spectroscopy , Peroxidases/chemistry , Chemical Phenomena , Chemistry, Physical , Cyanides/chemistry , Cytochrome-c Peroxidase/chemistry , Horseradish Peroxidase/chemistry , Iron/chemistry , Molecular Structure , Oxidation-ReductionABSTRACT
Recombinant Phanerochaete chrysosporium lignin peroxidase isozyme H2 (pI 4.4) was produced in insect cells infected with a genetically engineered baculovirus containing a copy of the cDNA clone lambda ML-6. The recombinant enzyme was purified to near homogeneity and is capable of oxidizing veratryl alcohol, iodide, and, to a lesser extent, guaiacol. The Km of the recombinant enzyme for veratryl alcohol and H2O2 is similar to that of the fungal enzyme. The guaiacol oxidation activity or any other activity is not dependent upon Mn2+. The purified recombinant peroxidase is glycosylated with N-linked carbohydrate(s). The recombinant lignin peroxidase eluted from an anion exchange resin similar to that of native isozyme H1 rather than H2. However, the pI of the recombinant enzymes is different from both H1 and H2 isozymes. Further characterization of native isozymes H1 and H2 from the fungal cultures revealed identical N-terminus residues. This indicates that isozymes H1 and H2 differ in post-translational modification.
Subject(s)
Baculoviridae/genetics , Fungi/enzymology , Isoenzymes/biosynthesis , Peroxidases/biosynthesis , Amino Acid Sequence , Animals , Blotting, Southern , Blotting, Western , Cell Line , Cloning, Molecular , DNA/genetics , Genetic Vectors , Insecta , Isoelectric Focusing , Isoenzymes/chemistry , Isoenzymes/genetics , Molecular Sequence Data , Peroxidases/chemistry , Peroxidases/genetics , Recombinant Proteins/biosynthesis , TransfectionABSTRACT
Lignin and Mn peroxidases are two families of isozymes produced by the lignin-degrading fungus Phanerochaete chrysosporium under nutrient nitrogen or carbon limitation. We purified to homogeneity the three major Mn peroxidase isozymes, H3 (pI = 4.9), H4 (pI = 4.5), and H5 (pI = 4.2). Amino-terminal sequencing of these isozymes demonstrates that they are encoded by different genes. We also analyzed the regulation of these isozymes in carbon- and nitrogen-limited cultures and found not only that the lignin and Mn peroxidases are differentially regulated but also that differential regulation occurs within the Mn peroxidase isozyme family. The isozyme profile and the time at which each isozyme appears in secondary metabolism differ in both nitrogen- and carbon-limited cultures. Each isozyme also responded differently to the addition of a putative inducer, divalent Mn. The stability of the Mn peroxidases in carbon- and nitrogen-limited cultures was also characterized after cycloheximide addition. The Mn peroxidases are more stable in carbon-limited cultures than in nitrogen-limited cultures. They are also more stable than the lignin peroxidases. These data collectively suggest that the Mn peroxidase isozymes serve different functions in lignin biodegradation.
Subject(s)
Fungi/enzymology , Isoenzymes/metabolism , Peroxidases/metabolism , Amino Acid Sequence , Amino Acids/analysis , Culture Media/pharmacology , Enzyme Stability , Gene Expression Regulation, Fungal/drug effects , Kinetics , Manganese/pharmacology , Molecular Sequence Data , RNA, Messenger/analysis , Sequence Homology, Nucleic AcidABSTRACT
The cDNA encoding Mn peroxidase isozyme H4 from Phanerochaete chrysosporium was recombined into a baculovirus and heterologously expressed in Sf9 cells. The recombinant Mn peroxidase has the same molecular weight as the native enzyme as determined by SDS-PAGE and cross-reacts with a Mn peroxidase-specific antibody. The recombinant enzyme has a slightly lower pI than the native fungal isozyme H4 indicating some differences in post-translational modification. Phenol red, guaiacol, and vanillylacetone, substrates of the native Mn peroxidase, are oxidized by the recombinant enzyme. All of the activities are dependent on both Mn (II) and H2O2.
Subject(s)
Peroxidases/genetics , Baculoviridae/genetics , Chrysosporium/enzymology , DNA, Fungal/analysis , Electrophoresis, Polyacrylamide Gel , Gene Expression , Isoelectric Point , Isoenzymes/genetics , Molecular Weight , Peroxidases/biosynthesis , Protein Processing, Post-Translational , Recombinant Proteins/biosynthesis , Recombinant Proteins/genetics , Substrate SpecificityABSTRACT
A cDNA clone encoding a manganese-dependent peroxidase from the filamentous fungus Phanerochaete chrysosporium was isolated and characterized. The clone, lambda MP-1, was isolated by screening a lambda gt11 expression library with polyclonal antibodies raised against a purified manganese-dependent peroxidase (isozyme H4, pI 4.5). The lambda MP-1 cDNA sequence predicts a mature protein containing 358 amino acids with a molecular weight of 37,711 preceded by a leader peptide of 24 amino acid residues. The N-terminal amino acid sequence of a purified manganese-dependent peroxidase (H4) corresponds to the sequence deduced from the cDNA. Some homology (58% in nucleotide sequence and 65% in amino acid sequence) is observed between the manganese-dependent peroxidase and lignin peroxidase isozyme H8. The highest degree of similarity is observed near the enzyme active site. Residues essential for peroxidase activity, the distal and proximal histidines, can be identified in the amino acid sequence. Near these residues, homology is also observed with several other peroxidases. Northern blot analysis of poly(A)+ RNA from nitrogen-limited P. chrysosporium cultures indicates that the level of messenger RNA correlates with expression of the enzyme and its activity. This is consistent with the regulation of the enzyme being at the level of transcription.
Subject(s)
Agaricales/enzymology , DNA, Fungal/genetics , Peroxidases/genetics , Agaricales/genetics , Agaricales/growth & development , Amino Acid Sequence , Base Sequence , Genes , Genes, Fungal , Manganese/pharmacology , Molecular Sequence Data , Molecular Weight , Peroxidases/metabolism , Restriction MappingABSTRACT
Two cDNA clones encoding lignin peroxidase isozymes from Phanerochaete chrysosporium have been isolated and characterized. One of the clones, lambda ML-4, encodes isozyme H8 as does the previously reported clone lambda ML-1 [Tien, M. and Tu, C.-P.D. Nature 326 (1987) 520-523; 328, 742]. Our data are consistent with lambda ML-1 and lambda ML-4 being allelic variants. The other clone, lambda ML-5, encodes a homologous isozyme. We have also isolated the genomic clone corresponding to lambda ML-4 cDNA. Conserved residues thought to be essential for peroxidase function were identified in the predicted amino acid sequences of both cDNA clones. Northern blot analyses indicate that these isozymes are expressed during secondary metabolism, appearing on day 4 of growth and increasing on days 5 and 6.