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1.
Science ; 354(6318): 1431-1433, 2016 12 16.
Article in English | MEDLINE | ID: mdl-27980209

ABSTRACT

Ski2-Ski3-Ski8 (Ski) is a helicase complex functioning with the RNA-degrading exosome to mediate the 3'-5' messenger RNA (mRNA) decay in turnover and quality-control pathways. We report that the Ski complex directly associates with 80S ribosomes presenting a short mRNA 3' overhang. We determined the structure of an endogenous ribosome-Ski complex using cryo-electron microscopy (EM) with a local resolution of the Ski complex ranging from 4 angstroms (Å) in the core to about 10 Å for intrinsically flexible regions. Ribosome binding displaces the autoinhibitory domain of the Ski2 helicase, positioning it in an open conformation near the ribosomal mRNA entry tunnel. We observe that the mRNA 3' overhang is threaded directly from the small ribosomal subunit to the helicase channel of Ski2, primed for ongoing exosome-mediated 3'-5' degradation.


Subject(s)
DNA Helicases/ultrastructure , Exosome Multienzyme Ribonuclease Complex/ultrastructure , Nuclear Proteins/ultrastructure , RNA Stability , Ribosome Subunits, Large, Eukaryotic/ultrastructure , Saccharomyces cerevisiae Proteins/ultrastructure , Saccharomyces cerevisiae/enzymology , Cryoelectron Microscopy , Protein Conformation , RNA, Fungal/metabolism , RNA, Messenger/metabolism , RNA, Ribosomal/metabolism , Ribosome Subunits, Large, Eukaryotic/enzymology
2.
Nat Struct Mol Biol ; 23(12): 1074-1082, 2016 Dec.
Article in English | MEDLINE | ID: mdl-27775710

ABSTRACT

Nuclear export of preribosomal subunits is a key step during eukaryotic ribosome formation. To efficiently pass through the FG-repeat meshwork of the nuclear pore complex, the large pre-60S subunit requires several export factors. Here we describe the mechanism of recruitment of the Saccharomyces cerevisiae RNA-export receptor Mex67-Mtr2 to the pre-60S subunit at the proper time. Mex67-Mtr2 binds at the premature ribosomal-stalk region, which later during translation serves as a binding platform for translational GTPases on the mature ribosome. The assembly factor Mrt4, a structural homolog of cytoplasmic-stalk protein P0, masks this site, thus preventing untimely recruitment of Mex67-Mtr2 to nuclear pre-60S particles. Subsequently, Yvh1 triggers Mrt4 release in the nucleus, thereby creating a narrow time window for Mex67-Mtr2 association at this site and facilitating nuclear export of the large subunit. Thus, a spatiotemporal mark on the ribosomal stalk controls the recruitment of an RNA-export receptor to the nascent 60S subunit.


Subject(s)
Membrane Transport Proteins/metabolism , Nuclear Proteins/metabolism , Nucleocytoplasmic Transport Proteins/metabolism , RNA-Binding Proteins/metabolism , Ribosome Subunits, Large/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Active Transport, Cell Nucleus , Base Sequence , Binding Sites , Membrane Transport Proteins/chemistry , Models, Molecular , Nuclear Proteins/chemistry , Nucleocytoplasmic Transport Proteins/chemistry , Protein Binding , RNA Transport , RNA, Fungal/chemistry , RNA, Fungal/metabolism , RNA-Binding Proteins/chemistry , Ribosome Subunits, Large/chemistry , Saccharomyces cerevisiae/chemistry , Saccharomyces cerevisiae Proteins/chemistry
3.
Proc Natl Acad Sci U S A ; 113(9): E1180-9, 2016 Mar 01.
Article in English | MEDLINE | ID: mdl-26888283

ABSTRACT

According to the standard model of bacterial translation initiation, the small ribosomal 30S subunit binds to the initiation site of an mRNA with the help of three initiation factors (IF1-IF3). Here, we describe a novel type of initiation termed "70S-scanning initiation," where the 70S ribosome does not necessarily dissociate after translation of a cistron, but rather scans to the initiation site of the downstream cistron. We detailed the mechanism of 70S-scanning initiation by designing unique monocistronic and polycistronic mRNAs harboring translation reporters, and by reconstituting systems to characterize each distinct mode of initiation. Results show that 70S scanning is triggered by fMet-tRNA and does not require energy; the Shine-Dalgarno sequence is an essential recognition element of the initiation site. IF1 and IF3 requirements for the various initiation modes were assessed by the formation of productive initiation complexes leading to synthesis of active proteins. IF3 is essential and IF1 is highly stimulating for the 70S-scanning mode. The task of IF1 appears to be the prevention of untimely interference by ternary aminoacyl (aa)-tRNA•elongation factor thermo unstable (EF-Tu)•GTP complexes. Evidence indicates that at least 50% of bacterial initiation events use the 70S-scanning mode, underscoring the relative importance of this translation initiation mechanism.


Subject(s)
Protein Biosynthesis , Ribosomes/metabolism , Models, Molecular , Peptide Initiation Factors/metabolism , RNA, Messenger/metabolism
4.
Nucleic Acids Res ; 44(4): 1944-51, 2016 Feb 29.
Article in English | MEDLINE | ID: mdl-26715760

ABSTRACT

During protein synthesis, ribosomes become stalled on polyproline-containing sequences, unless they are rescued in archaea and eukaryotes by the initiation factor 5A (a/eIF-5A) and in bacteria by the homologous protein EF-P. While a structure of EF-P bound to the 70S ribosome exists, structural insight into eIF-5A on the 80S ribosome has been lacking. Here we present a cryo-electron microscopy reconstruction of eIF-5A bound to the yeast 80S ribosome at 3.9 Å resolution. The structure reveals that the unique and functionally essential post-translational hypusine modification reaches toward the peptidyltransferase center of the ribosome, where the hypusine moiety contacts A76 of the CCA-end of the P-site tRNA. These findings would support a model whereby eIF-5A stimulates peptide bond formation on polyproline-stalled ribosomes by stabilizing and orienting the CCA-end of the P-tRNA, rather than by directly contributing to the catalysis.


Subject(s)
Peptide Initiation Factors/chemistry , Protein Biosynthesis/genetics , RNA-Binding Proteins/chemistry , Ribosomes/chemistry , Cryoelectron Microscopy , Peptide Elongation Factors/chemistry , Peptide Elongation Factors/metabolism , Peptide Initiation Factors/metabolism , Peptides/genetics , Protein Processing, Post-Translational/genetics , RNA, Messenger/chemistry , RNA, Messenger/metabolism , RNA-Binding Proteins/metabolism , Ribosomes/metabolism , Saccharomyces cerevisiae/chemistry , Saccharomyces cerevisiae/genetics , Eukaryotic Translation Initiation Factor 5A
5.
Nat Commun ; 6: 8403, 2015 Sep 28.
Article in English | MEDLINE | ID: mdl-26411746

ABSTRACT

In mammalian cells, secretory and membrane proteins are translocated across or inserted into the endoplasmic reticulum (ER) membrane by the universally conserved protein-conducting channel Sec61, which has been structurally studied in isolated, detergent-solubilized states. Here we structurally and functionally characterize native, non-solubilized ribosome-Sec61 complexes on rough ER vesicles using cryo-electron tomography and ribosome profiling. Surprisingly, the 9-Å resolution subtomogram average reveals Sec61 in a laterally open conformation, even though the channel is not in the process of inserting membrane proteins into the lipid bilayer. In contrast to recent mechanistic models for polypeptide translocation and insertion, our results indicate that the laterally open conformation of Sec61 is the only conformation present in the ribosome-bound translocon complex, independent of its functional state. Consistent with earlier functional studies, our structure suggests that the ribosome alone, even without a nascent chain, is sufficient for lateral opening of Sec61 in a lipid environment.


Subject(s)
Membrane Proteins/chemistry , Animals , Dogs , Humans , Membrane Proteins/metabolism , Protein Conformation , Ribosomes/metabolism , SEC Translocation Channels
6.
Chembiochem ; 13(12): 1791-7, 2012 Aug 13.
Article in English | MEDLINE | ID: mdl-22865768

ABSTRACT

Previously we have shown that the CCA end of a P-tRNA can be crosslinked with the RPL36AL protein of the large subunit of mammalian ribosomes; it belongs to the L44e protein family present in all eukaryotic and archaeal ribosomes. Here we confirm and extend this finding and demonstrate that: 1) this crosslink is specific for a tRNA at the P/E hybrid site, as a tRNA in all other tRNA positions of pre-translocational ribosomes could not be crosslinked with a ribosomal protein, 2) the crosslink was formed most efficiently with C74 and C75 of P/E-tRNA, but could also connect the ultimate A of this tRNA with Lys53 of protein RPL36AL, 3) this protein contains seven monomethylated residues (three lysyl and three arginyl residues, as well as glutaminyl residue 51), 4) Q51 is part of a conserved GGQ motif in the L44e proteins in eukaryotic 80S ribosomes that is identical to the universally conserved motif of release factors implicated in promoting peptidyl-tRNA hydrolysis, and 5) the large number of modifications, in which some of the residues were methylated to about 50 %, might indicate that protein RPL36AL is a preferential target for regulation.


Subject(s)
Lysine/chemistry , Protein Biosynthesis , RNA, Transfer, Amino Acyl/chemistry , Ribosomal Proteins/chemistry , Ribosomes/chemistry , Amino Acid Motifs , Animals , Binding Sites , Cattle , Cross-Linking Reagents , Humans , Lysine/metabolism , Models, Molecular , Nucleic Acid Conformation , Protein Binding , RNA, Transfer, Amino Acyl/metabolism , Ribosomal Proteins/metabolism , Ribosomes/metabolism , Saccharomyces cerevisiae , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
7.
Mol Microbiol ; 86(1): 6-9, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22909071

ABSTRACT

The tmRNA/SmpB system, which is almost universal in bacteria, rescues bacterial ribosomes stalled at the end of non-stop mRNAs (mRNAs lacking a stop codon). In addition, a few bacteria, including Escherichia coli, have developed a second two-component system as reported by Chadani et al. (2012). A small protein, ArfA of 55 amino acids (formerly called YdhL), mediates binding of release factor 2 to the ribosomal A site lacking a complete mRNA codon and thereby triggers translational termination and rescue of the stalled ribosome.


Subject(s)
Escherichia coli Proteins/metabolism , Escherichia coli/enzymology , Escherichia coli/metabolism , Peptide Termination Factors/metabolism , RNA, Transfer, Amino Acyl/metabolism , RNA-Binding Proteins/metabolism , Ribosomes/metabolism
9.
PLoS Genet ; 8(7): e1002815, 2012.
Article in English | MEDLINE | ID: mdl-22829778

ABSTRACT

The YbeB (DUF143) family of uncharacterized proteins is encoded by almost all bacterial and eukaryotic genomes but not archaea. While they have been shown to be associated with ribosomes, their molecular function remains unclear. Here we show that YbeB is a ribosomal silencing factor (RsfA) in the stationary growth phase and during the transition from rich to poor media. A knock-out of the rsfA gene shows two strong phenotypes: (i) the viability of the mutant cells are sharply impaired during stationary phase (as shown by viability competition assays), and (ii) during transition from rich to poor media the mutant cells adapt slowly and show a growth block of more than 10 hours (as shown by growth competition assays). RsfA silences translation by binding to the L14 protein of the large ribosomal subunit and, as a consequence, impairs subunit joining (as shown by molecular modeling, reporter gene analysis, in vitro translation assays, and sucrose gradient analysis). This particular interaction is conserved in all species tested, including Escherichia coli, Treponema pallidum, Streptococcus pneumoniae, Synechocystis PCC 6803, as well as human mitochondria and maize chloroplasts (as demonstrated by yeast two-hybrid tests, pull-downs, and mutagenesis). RsfA is unrelated to the eukaryotic ribosomal anti-association/60S-assembly factor eIF6, which also binds to L14, and is the first such factor in bacteria and organelles. RsfA helps cells to adapt to slow-growth/stationary phase conditions by down-regulating protein synthesis, one of the most energy-consuming processes in both bacterial and eukaryotic cells.


Subject(s)
Bacteria , Eukaryota , Ribosomal Proteins/chemistry , Ribosome Subunits, Large/chemistry , Bacteria/genetics , Bacteria/growth & development , Bacteria/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Binding Sites , Conserved Sequence , Eukaryota/genetics , Eukaryota/growth & development , Eukaryota/metabolism , HeLa Cells , Humans , Mitochondria/genetics , Mitochondria/metabolism , Molecular Sequence Data , Phylogeny , Protein Binding , Protein Biosynthesis/genetics , Ribosomal Proteins/metabolism , Ribosome Subunits, Large/metabolism , Sequence Homology, Amino Acid , Transcription Factors/genetics , Transcription Factors/metabolism
10.
Nature ; 485(7399): 526-9, 2012 May 06.
Article in English | MEDLINE | ID: mdl-22622583

ABSTRACT

Bacterial ribosomes stalled at the 3' end of malfunctioning messenger RNAs can be rescued by transfer-messenger RNA (tmRNA)-mediated trans-translation. The SmpB protein forms a complex with the tmRNA, and the transfer-RNA-like domain (TLD) of the tmRNA then enters the A site of the ribosome. Subsequently, the TLD-SmpB module is translocated to the P site, a process that is facilitated by the elongation factor EF-G, and translation is switched to the mRNA-like domain (MLD) of the tmRNA. Accurate loading of the MLD into the mRNA path is an unusual initiation mechanism. Despite various snapshots of different ribosome-tmRNA complexes at low to intermediate resolution, it is unclear how the large, highly structured tmRNA is translocated and how the MLD is loaded. Here we present a cryo-electron microscopy reconstruction of a fusidic-acid-stalled ribosomal 70S-tmRNA-SmpB-EF-G complex (carrying both of the large ligands, that is, EF-G and tmRNA) at 8.3 Å resolution. This post-translocational intermediate (TI(POST)) presents the TLD-SmpB module in an intrasubunit ap/P hybrid site and a tRNA(fMet) in an intrasubunit pe/E hybrid site. Conformational changes in the ribosome and tmRNA occur in the intersubunit space and on the solvent side. The key underlying event is a unique extra-large swivel movement of the 30S head, which is crucial for both tmRNA-SmpB translocation and MLD loading, thereby coupling translocation to MLD loading. This mechanism exemplifies the versatile, dynamic nature of the ribosome, and it shows that the conformational modes of the ribosome that normally drive canonical translation can also be used in a modified form to facilitate more complex tasks in specialized non-canonical pathways.


Subject(s)
Escherichia coli/chemistry , Peptide Elongation Factor G/metabolism , Protein Biosynthesis , RNA, Bacterial/chemistry , RNA, Bacterial/metabolism , RNA-Binding Proteins/metabolism , Ribosomes/metabolism , Base Sequence , Cryoelectron Microscopy , Fusidic Acid/metabolism , Ligands , Models, Molecular , Nucleic Acid Conformation , Peptide Elongation Factor G/chemistry , Peptide Elongation Factor G/ultrastructure , Protein Binding , Protein Conformation , RNA, Bacterial/genetics , RNA, Bacterial/ultrastructure , RNA-Binding Proteins/chemistry , RNA-Binding Proteins/ultrastructure , Ribosome Subunits/chemistry , Ribosome Subunits/genetics , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Ribosomes/chemistry , Ribosomes/genetics , Ribosomes/ultrastructure
13.
Proc Natl Acad Sci U S A ; 108(8): 3199-203, 2011 Feb 22.
Article in English | MEDLINE | ID: mdl-21300907

ABSTRACT

Elongation factor 4 (EF4) is one of the most conserved proteins present in bacteria as well as in mitochondria and chloroplasts of eukaryotes. Although EF4 has the unique ability to catalyze the back-translocation reaction on posttranslocation state ribosomes, the physiological role of EF4 remains unclear. Here we demonstrate that EF4 is stored at the membrane of Escherichia coli cells and released into the cytoplasm upon conditions of high ionic strength or low temperature. Under such conditions, wild-type E. coli cells overgrow mutant cells lacking the EF4 gene within 5-10 generations. Elevated intracellular Mg(2+) concentrations or low temperature retard bacterial growth and inhibit protein synthesis, probably because of formation of aberrant elongating ribosomal states. We suggest that EF4 binds to these stuck ribosomes and remobilizes them, consistent with the EF4-dependent enhancement (fivefold) in protein synthesis observed under these unfavorable conditions. The strong selective advantage conferred by the presence of EF4 at high intracellular ionic strength or low temperatures explains the ubiquitous distribution and high conservation of EF4.


Subject(s)
Escherichia coli Proteins/metabolism , Magnesium , Protein Biosynthesis/genetics , Transcriptional Elongation Factors/metabolism , Escherichia coli/growth & development , Escherichia coli Proteins/physiology , Magnesium/pharmacology , Osmolar Concentration , Peptide Initiation Factors , Protein Transport , Ribosomes/pathology , Temperature , Transcriptional Elongation Factors/physiology
14.
Mol Biol Cell ; 22(1): 1-11, 2011 Jan 01.
Article in English | MEDLINE | ID: mdl-21118999

ABSTRACT

Nitric oxide associated-1 (NOA1) is an evolutionarily conserved guanosine triphosphate (GTP) binding protein that localizes predominantly to mitochondria in mammalian cells. On the basis of bioinformatic analysis, we predicted its possible involvement in ribosomal biogenesis, although this had not been supported by any experimental evidence. Here we determine NOA1 function through generation of knockout mice and in vitro assays. NOA1-deficient mice exhibit midgestation lethality associated with a severe developmental defect of the embryo and trophoblast. Primary embryonic fibroblasts isolated from NOA1 knockout embryos show deficient mitochondrial protein synthesis and a global defect of oxidative phosphorylation (OXPHOS). Additionally, Noa1⁻/⁻ cells are impaired in staurosporine-induced apoptosis. The analysis of mitochondrial ribosomal subunits from Noa1⁻/⁻ cells by sucrose gradient centrifugation and Western blotting showed anomalous sedimentation, consistent with a defect in mitochondrial ribosome assembly. Furthermore, in vitro experiments revealed that intrinsic NOA1 GTPase activity was stimulated by bacterial ribosomal constituents. Taken together, our data show that NOA1 is required for mitochondrial protein synthesis, likely due to its yet unidentified role in mitoribosomal biogenesis. Thus, NOA1 is required for such basal mitochondrial functions as adenosine triphosphate (ATP) synthesis and apoptosis.


Subject(s)
GTP Phosphohydrolases/metabolism , Mitochondria/metabolism , Mitochondrial Proteins/biosynthesis , Adenosine Triphosphate/biosynthesis , Animals , Apoptosis , Cells, Cultured , Embryo, Mammalian/abnormalities , Embryonic Development , Fetal Death , Fibroblasts , GTP Phosphohydrolases/genetics , Humans , In Situ Hybridization , Mice , Mice, Knockout , Oxidative Phosphorylation , Protein Biosynthesis/genetics , RNA, Small Interfering , Ribosomes/metabolism , Staurosporine/metabolism
15.
J Biol Chem ; 285(25): 19679-87, 2010 Jun 18.
Article in English | MEDLINE | ID: mdl-20410297

ABSTRACT

Nascent polypeptide-associated complex (NAC) was identified in eukaryotes as the first cytosolic factor that contacts the nascent polypeptide chain emerging from the ribosome. NAC is present as a homodimer in archaea and as a highly conserved heterodimer in eukaryotes. Mutations in NAC cause severe embryonically lethal phenotypes in mice, Drosophila melanogaster, and Caenorhabditis elegans. In the yeast Saccharomyces cerevisiae NAC is quantitatively associated with ribosomes. Here we show that NAC contacts several ribosomal proteins. The N terminus of betaNAC, however, specifically contacts near the tunnel exit ribosomal protein Rpl31, which is unique to eukaryotes and archaea. Moreover, the first 23 amino acids of betaNAC are sufficient to direct an otherwise non-associated protein to the ribosome. In contrast, alphaNAC (Egd2p) contacts Rpl17, the direct neighbor of Rpl31 at the ribosomal tunnel exit site. Rpl31 was also recently identified as a contact site for the SRP receptor and the ribosome-associated complex. Furthermore, in Escherichia coli peptide deformylase (PDF) interacts with the corresponding surface area on the eubacterial ribosome. In addition to the previously identified universal adapter site represented by Rpl25/Rpl35, we therefore refer to Rpl31/Rpl17 as a novel universal docking site for ribosome-associated factors on the eukaryotic ribosome.


Subject(s)
Peptides/chemistry , Ribosomes/chemistry , Amino Acids/chemistry , Animals , Chaperonins/chemistry , Cross-Linking Reagents/chemistry , Escherichia coli/metabolism , Humans , Mice , Mutation , Phenotype , Protein Interaction Mapping , Protein Structure, Tertiary , Ribosomal Proteins/chemistry
16.
J Mol Biol ; 396(4): 1043-52, 2010 Mar 05.
Article in English | MEDLINE | ID: mdl-20045415

ABSTRACT

EF4, although structurally similar to the translocase EF-G, promotes back-translocation of tRNAs on the ribosome and is important for bacterial growth under certain conditions. Here, using a coordinated set of in vitro kinetic measures, including changes in the puromycin reactivity of peptidyl-tRNA and in the fluorescence of labeled tRNAs and mRNA, we elucidate the kinetic mechanism of EF4-catalyzed back-translocation and determine the effects of the translocation inhibitors spectinomycin and viomycin on the process. EF4-dependent back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex via a four-step kinetic scheme (i.e., POST-->I(1)-->I(2)-->I(3)-->PRE, which is not the simple reverse of translocation). During back-translocation, movements of the tRNA core regions and of mRNA are closely coupled to one another but are sometimes decoupled from movement of the 3'-end of peptidyl-tRNA. EF4 may be thought of as performing an interrupted catalysis of back-translocation, stopping at the formation of I(3) rather than catalyzing the complete process of back-translocation culminating in PRE complex formation. The delay in polypeptide elongation resulting from transient accumulation of I(3) is likely to be important for optimizing functional protein biosynthesis.


Subject(s)
Escherichia coli Proteins/metabolism , Transcriptional Elongation Factors/metabolism , Base Sequence , Catalysis , Escherichia coli/genetics , Escherichia coli/metabolism , Escherichia coli Proteins/genetics , Kinetics , Models, Biological , Peptide Initiation Factors , Puromycin/metabolism , RNA, Bacterial/genetics , RNA, Bacterial/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Transfer, Amino Acyl/genetics , RNA, Transfer, Amino Acyl/metabolism , Ribosomes/metabolism , Spectrometry, Fluorescence , Transcriptional Elongation Factors/genetics
17.
Methods Mol Biol ; 488: 129-51, 2008.
Article in English | MEDLINE | ID: mdl-18982288

ABSTRACT

Analogs of naturally occurring substances obtained by chemical modifications are powerful tools to study intra- and intermolecular interactions. We have used the phosphorothioate technique to analyze RNA-protein interactions, here the interactions of transfer RNAs (tRNAs) with the three ribosomal binding sites. We describe preparation and purification of thioated tRNAs, formation of functional complexes of programmed ribosomes with tRNAs, and the evaluation of the observed phosphorothioate footprints on the tRNAs.


Subject(s)
Phosphates/chemistry , Protein Footprinting/methods , Proteins/chemistry , RNA/chemistry , Ribosomes/chemistry , Molecular Structure , Nucleic Acid Conformation , Protein Binding , Proteins/metabolism , RNA/metabolism , Ribosomes/metabolism
18.
Proc Natl Acad Sci U S A ; 105(31): 10715-20, 2008 Aug 05.
Article in English | MEDLINE | ID: mdl-18667704

ABSTRACT

During translation, usually only one in approximately 400 misincorporations affects the function of a nascent protein, because only chemically similar near-cognate amino acids are misincorporated in place of the cognate one. The deleterious misincorporation of a chemically dissimilar noncognate amino acid during the selection process is precluded by the presence of a tRNA at the ribosomal E-site. However, the selection of first aminoacyl-tRNA, directly after initiation, occurs without an occupied E-site, i.e., when only the P-site is filled with the initiator tRNA and thus should be highly error-prone. Here, we show how bacterial ribosomes have solved this accuracy problem: In the absence of a Shine-Dalgarno (SD) sequence, the first decoding step at the A-site after initiation is extremely error-prone, even resulting in the significant incorporation of noncognate amino acids. In contrast, when a SD sequence is present, the incorporation of noncognate amino acids is not observed. This is precisely the effect that the presence of a cognate tRNA at the E-site has during the elongation phase. These findings suggest that during the initiation phase, the SD interaction functionally compensates for the lack of codon-anticodon interaction at the E-site by reducing the misincorporation of near-cognate amino acids and prevents noncognate misincorporation.


Subject(s)
Amino Acids/metabolism , Codon/metabolism , Models, Molecular , Peptide Chain Initiation, Translational/genetics , RNA, Transfer, Amino Acyl/genetics , Ribosomes/metabolism , Amino Acids/genetics , Base Sequence , Codon/genetics , Molecular Sequence Data , RNA, Messenger/genetics
19.
Chem Biol ; 15(5): 417-9, 2008 May.
Article in English | MEDLINE | ID: mdl-18482692

ABSTRACT

In this issue of Chemistry & Biology, Lang et al. (2008) add an important step toward a molecular understanding of ribosomal peptide-bond formation: they unravel the involvement of an essential partner of the reaction, namely the 2'-OH group of the 23S rRNA nucleotide A2451.


Subject(s)
Peptides/metabolism , Ribosomal Proteins/metabolism , Models, Molecular , Peptides/chemistry , Protein Conformation , Ribosomal Proteins/chemistry
20.
J Biol Chem ; 280(16): 15849-54, 2005 Apr 22.
Article in English | MEDLINE | ID: mdl-15665334

ABSTRACT

Nascent polypeptide-associated complex (NAC) was identified in eukaryotes as the first cytosolic factor that contacts the nascent polypeptide chain emerging from the ribosome. NAC is highly conserved from yeast to humans. Mutations in NAC cause severe embryonically lethal phenotypes in mice, Drosophila, and Caenorhabditis elegans. NAC was suggested to protect the nascent chain from inappropriate early interactions with cytosolic factors. Eukaryotic NAC is a heterodimer with two subunits sharing substantial homology with each other. All sequenced archaebacterial genomes exhibit only one gene homologous to the NAC subunits. Here we present the first archaebacterial NAC homolog. It forms a homodimer, and as eukaryotic NAC it is associated with ribosomes and contacts the emerging nascent chain on the ribosome. We present the first crystal structure of a NAC protein revealing two structural features: (i) a novel unique protein fold that mediates dimerization of the complex, and (ii) a ubiquitin-associated domain that suggests a yet unidentified role for NAC in the cellular protein quality control system via the ubiquitination pathway. Based on the presented structure we propose a model for the eukaryotic heterodimeric NAC domain.


Subject(s)
Methanobacteriaceae/metabolism , Trans-Activators/chemistry , Ubiquitin/metabolism , Crystallography, X-Ray , Molecular Chaperones , Protein Structure, Tertiary , Ribosomes/metabolism , Trans-Activators/metabolism
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