ABSTRACT
Marigolds (Tagetes erecta L.) were evaluated for phytoremediation potential of cadmium (Cd) and zinc (Zn) as a function of amendment application to soil. Vermicompost (V), biodigestate (Bi), and combined V + Bi (VBi) were used as soil amendments in Zn and Cd co-contaminated soils. Application of soil amendments can alter physicochemical properties of soils, particularly pH, EC, CEC and nutrient concentrations. The VBi treatment resulted in highest percentage growth rate in biomass (52 %) for the Twenty yellow variety of marigold. Also, in the VBi treatment, leaves of Dragon yellow variety exhibited maximal accumulation of Zn and Cd. Flower extracts of Twenty yellow in the V treatment had substantial carotenoid content (71.7 mg L-1) and lowest IC50 value (43.7 mg L-1), thus indicating it had highest DPPH free radical scavenging activity. Dragon yellow exhibited highest values of ferric reducing antioxidant power (FRAP; 2066 mg L-1), total flavonoids content (TFC; 64.1 mg L-1), and total phenolics content (TPC; 50.9 mg L-1). Using X-ray fluorescence (XRF) spectroscopy, the atomic percentages of Zn and Cd in all marigold varieties and treatments showed similar patterns over flower surfaces, seeds, and flower petals in descending order. Prime yellow in the V treatment resulted in higher Zn accumulation in roots (bioconcentration factor of root value) > 1 and translocation factor value < 1, indicating an enhanced ability of the plant for phytostabilization. Application of V altered antioxidant activities and production of bioactive compounds as well as enhanced the excluder potential of Cd and Zn, particularly in the Prime yellow variety. Application of Bi contributed to increased flower numbers, suggesting that floriculturists cultivating marigolds for ornamental purposes may be able to generate revenue in terms of productivity and quality of flowers when marigolds are grown on contaminated land.
