[Immunohistochemical assay of murine liver tissue using monoclonal antibodies to NO-synthase 2 and TNF-α under tetrachloromethane toxic injury conditions].

Experimental data showing a significant role of enzyme NO-synthase type 2 and cytokine TNF-α enzymes in pathogenesis of CCl4-induced liver injury have been obtained by immunohistochemical assay. It is established that the hepatotoxic agent leads to an increase in NO-synthase 2 and TNF-α levels. The use of both studied phytoadaptogens (eleutherococcus extract and ginseng tincture) at a doses of 48.5 mg/kg and 47 mg/kg, respectively, and the reference drug karsil (100 mg/kg daily for 5 weeks) prevented to a significant degree the accumulation of enzyme NO-synthase 2 and the cytokine TNF-α, which is apparently one of the mechanisms of hepatoprotective action.