Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 11 de 11
Filter
2.
J Periodontol ; 70(1): 8-12, 1999 Jan.
Article in English | MEDLINE | ID: mdl-10052766

ABSTRACT

BACKGROUND: Class II furcation lesions are a challenging scenario for periodontal therapy and a serious threat for tooth prognosis. Guided tissue regeneration represents a viable treatment option, but some aspects remain to be clarified as to the efficacy of bone substitutes used in GTR procedures. The clinical efficacy of demineralized freeze-dried bone allografts (DFDBA) for regenerative procedures is presently under scrutiny. METHODS: This study evaluated the adjunctive clinical effects of DFDBA associated with a bioabsorbable (polylactic acid) barrier membrane in the regenerative treatment of human Class II furcation lesions as compared to the same type of barrier alone. Twelve patients with bilateral Class II furcation defects on lower first molars participated in the study. In each patient, one tooth was randomly assigned to treatment consisting of open surgical debridement followed by the placement of DFDBA (GTR+DFDBA, or test group) and a bioabsorbable barrier, while the contralateral side received the same flap surgery followed by use of the bioabsorbable membrane alone (GTR, or control group). The clinical efficacy of the 2 treatment modalities was evaluated at 6 and 12 months postoperatively. Measurements included vertical probing depth (VPD), horizontal probing depth (HPD), clinical attachment level (CAL), amount of gingival recession (GR), and change in class of clinically detectable furcation involvement (FC). RESULTS: Both treatments yielded significant improvements for all clinical parameters between baseline and 6 and 12 months (P<0.05). The comparison between test and control groups revealed significantly greater reduction in HPD (P=0.01) and higher values of GR (P=0.008) for the test group. The mean gain in HPD for test sites was 2.3 mm, while it amounted to 1.7 mm for controls. A significantly greater proportion of test sites could be classified postoperatively as Class I defects compared to controls (91% versus 50%, respectively). CONCLUSIONS: The results of this study indicate that significant improvements from baseline occurred in VPD, HPD, CAL, and clinically detectable furcation involvement after the treatment of Class II furcation lesions with a bioabsorbable membrane with or without the adjunctive use of DFDBA. In addition, the placement of DFDBA in the furcation defect under the bioabsorbable membrane resulted in a greater mean reduction of horizontal probing depth when compared to the regenerative therapy alone.


Subject(s)
Absorbable Implants , Bone Transplantation , Furcation Defects/surgery , Guided Tissue Regeneration, Periodontal/methods , Membranes, Artificial , Adult , Aged , Analysis of Variance , Decalcification Technique , Female , Freeze Drying , Humans , Lactic Acid , Male , Middle Aged , Periodontal Index , Polyesters , Polymers , Treatment Outcome
3.
J Clin Periodontol ; 24(9 Pt 1): 610-7, 1997 Sep.
Article in English | MEDLINE | ID: mdl-9378831

ABSTRACT

The aim of the present study was: (1) to assess longitudinally the occurrence of Actinobacillus actinomycetemcomitans (Aa) in young subjects wearing fixed orthodontic appliances compared to matched appliance-free controls; (2) to determine whether the presence of the micro-organism at baseline could influence the periodontal status assessed 3 years later. 70 subjects, 27 male and 43 female, aged between 12 and 20 years participated in the study: 35 subjects under orthodontic treatment with fixed appliances for at least 6 months, and 35 appliance-free individuals matched for age and gender. All subjects were free of clinically demonstrable loss of attachment. They all received oral hygiene instructions 2x during the 2 months preceding the first clinical and microbiological examination. No subgingival instrumentation was performed between baseline and the 3-year examination. Clinical parameters included gingival bleeding index (GBI), pocket probing depth (PPD) and measurements of attachment level (AL). Statistically significant differences were reported regarding frequency of detection of Aa between both groups at each examination. The %s of orthodontic subjects infected with Aa at the baseline and at the 3-year examination were 86% and 80%, respectively, while the corresponding figures for control subjects were 16.6% and 26.6%. The frequency distribution of %s of Aa in the total anaerobic subgingival flora among control subjects remained fairly stable, whereas the proportion of orthodontic subjects yielding Aa at a concentration > or = 1.0% dropped significantly from 32% at baseline to 19% at the 3-year visit. Calculations of the relative risk for increasing GBI and PPD in both groups when Aa was present at baseline, revealed that the orthodontic subjects positive for Aa had a negligible relative risk of experiencing worse periodontal conditions compared to orthodontic patients where Aa was not detected at baseline. In contrast, control subjects initially infected with Aa presented with a risk for increased GBI 6.6x higher than that for subjects without Aa. In conclusion, the present study confirmed previous cross-sectional findings reporting that young individual with an integer periodontium wearing fixed orthodontic appliances harbor Aa with a statistically significant greater frequency than appliance-free matched controls. However, although orthodontic patients exhibited more inflammation, their deteriorated clinical conditions could not be accounted for by the sole presence of Aa in their sulci. In contrast, appliance-free young subjects initially infected with Aa had a higher risk of experiencing more gingival inflammation than subjects without the bacterium during a 3-year observation period.


Subject(s)
Aggregatibacter actinomycetemcomitans/physiology , Orthodontic Appliances , Orthodontics, Corrective , Periodontium/microbiology , Actinobacillus Infections/pathology , Adolescent , Adult , Aggregatibacter actinomycetemcomitans/isolation & purification , Bacteria, Anaerobic/isolation & purification , Case-Control Studies , Child , Cross-Sectional Studies , Female , Follow-Up Studies , Gingival Hemorrhage/microbiology , Gingival Hemorrhage/pathology , Health Education, Dental , Humans , Longitudinal Studies , Male , Oral Hygiene , Patient Education as Topic , Periodontal Attachment Loss/microbiology , Periodontal Attachment Loss/pathology , Periodontal Diseases/microbiology , Periodontal Diseases/pathology , Periodontal Index , Periodontal Pocket/microbiology , Periodontal Pocket/pathology , Periodontitis/microbiology , Periodontitis/pathology , Periodontium/pathology , Risk Factors
4.
J Periodontol ; 68(1): 18-23, 1997 Jan.
Article in English | MEDLINE | ID: mdl-9029447

ABSTRACT

The aim of this study was to assess by means of an ELISA technique, the occurrence of 3 putative periodontopathogens, Porphyromonas gingivalis, Bacteroides forsythus, and Treponema denticola, in 3 clinically-defined adult periodontal conditions. Thirty systemically-healthy subjects were selected and grouped into 3 categories according to their periodontal health: 1) 10 periodontitis subjects (PS), having moderate adult chronic periodontitis; 2) 10 untreated gingivitis subjects (UGS), exhibiting no signs of periodontal destruction but presenting with clinical signs of mild gingivitis; and, 3) 10 treated gingivitis subjects (TGS), having the same clinical status as UGS, but who received a thorough prophylaxis treatment within the past 7 to 14 days prior to the baseline examination. A total of 60 samples were collected subgingivally from the six Ramfjord teeth per subject in each group and ELISA analysis was carried out to give a semiquantitative estimate of P. gingivalis. B. forsythus, and T. denticola. The immunologic detection method suggested the presence of antigens of P. gingivalis, B. forsythus, and T. denticola in subjects from each of the 3 groups. When a global analysis for the 3 disease groups was performed at one time, statistically significant differences were found among the ELISA scores of the 3 bacterial species. For example, comparisons of the ELISA scores showed that the concentrations of P. gingivalis differed significantly when comparing TGS to UGS and PS, but not when examining UGS/PS. The ELISA scores for B. forsythus were significantly different between TGS and PS. Mean concentrations of T. denticola were significantly different when comparing PS to TGS or UGS, whereas no difference was found between the latter categories. Within the limited scope of this study, the concentration of antigens detectable from putative periodontopathogens like P. gingivalis, B. forsythus, and T. denticola differed among the 3 diseased groups, with periodontitis subjects often showing the greatest level of antigens. Thus, it is reasonable to expect that, when using sensitive immunological detection methods, antigens of suspected periodontal pathogens can be found irrespective of the individual's clinical status. However, while detectable in the periodontal sites, the concentrations of these microorganisms are most likely to be above the threshold necessary to induce clinically-significant disease. Studies with larger sample size and standardized antigens are necessary to determine if the groups we found not to differ, were, in fact, different.


Subject(s)
Bacteroides/isolation & purification , Gingivitis/microbiology , Periodontitis/microbiology , Porphyromonas gingivalis/isolation & purification , Treponema/isolation & purification , Adult , Aged , Analysis of Variance , Antigens, Bacterial/analysis , Biomarkers , Chronic Disease , Colony Count, Microbial , Dental Plaque Index , Enzyme-Linked Immunosorbent Assay , Female , Humans , Male , Middle Aged , Periodontal Index , Statistics, Nonparametric
5.
Quintessence Int ; 27(6): 395-400, 1996 Jun.
Article in English | MEDLINE | ID: mdl-8941833

ABSTRACT

This study evaluated the 6-month clinical response to sustained-release tetracycline fibers used alone or with scaling and root planing in 25 adult periodontal maintenance patients. All subjects had at least one pocket > or = 4.0 mm that bled on probing and required therapy. Thirty-six teeth were treated with tetracycline fibers for 7 to 12 days; twelve of the 36 teeth also received scaling and root planing. The selection of teeth for scaling and root planing was based on the condition of the teeth. Therapeutic results were evaluated by changes in probing depth and frequency of bleeding on probing. Use of tetracycline fibers and fibers with scaling produced 1.8- and 1.7-mm reductions in probing depth, respectively, 1 month after treatment; reductions declined to 1.3 and 0.8 mm at 3 months, but rebounded to 1.5 and 1.3 mm at 6 months. The percentage of teeth exhibiting bleeding on probing decreased from 100% at baseline to 68% and 50% in the fiber and fiber plus scaling groups, respectively, at 6 months. None of the differences was statistically significant. Tetracycline fibers clearly decreased clinical signs of periodontal inflammation. Addition of scaling and root planing at the time of fiber placement further decreased, although not significantly, the degree of inflammation.


Subject(s)
Anti-Bacterial Agents/administration & dosage , Periodontal Diseases/therapy , Tetracycline/administration & dosage , Adult , Delayed-Action Preparations , Dental Scaling , Humans , Periodontal Diseases/drug therapy , Periodontal Index , Periodontal Pocket/drug therapy , Periodontal Pocket/therapy , Polyvinyls , Root Planing
6.
J Clin Periodontol ; 23(2): 112-8, 1996 Feb.
Article in English | MEDLINE | ID: mdl-8849847

ABSTRACT

The aim of the present study was to assess: (1) the occurrence of Actinobacillus actinomycetemcomitans (Aa) in subgingival plaque from young patients undergoing orthodontic treatment with fixed appliances; (2) a possible relationship between the presence of Aa and the clinical conditions; (3) a relation between the duration of orthodontic treatment and the microbiological and clinical parameters; (4) whether differences exist when taking into consideration the different type of appliances, i.e., bands or brackets. 34 subjects aged between 12 and 20 years participated in the study. Of these, 20 subjects had worn orthodontic appliances (test group), while the remaining 14 subjects served as matched control (control group). 4 to 8 sites in each patient were available for clinical and microbiological examination. Clinical parameters consisted of presence/absence of plaque and gingival bleeding index (GBI). Microbiological sampling was performed in the same sites as in the clinical examination. A statistically significant difference was present when comparing %s of GBI positive scores between teeth from the test group (57.5%) and teeth from the control group (25%). Plaque was present in 53% of test sites and 37% of control sites, but this difference was not statistically significant. Aa was detected from at least one site in 85% of test subjects and in 15% of the control subjects (p < 0.001). Among the subjects, 41% harboured Aa at a concentration between 0.1% and 1.0%, whereas another 40% yielded Aa at a concentration greater than 1.0%. Finally, a positive correlation was noted between the % of sites positive for Aa and the % of sites displaying a positive GBI score (r = 0.41; p < 0.005). No relation was found between the duration of orthodontic treatment and the microbiological or clinical parameters; neither were statistically significant differences found when we compared results from sites wearing bands or brackets. In conclusion, the present study showed that young subjects wearing orthodontic appliances harbour Aa with a remarkable frequency of detection, although plaque levels do not significantly differ from those of a matched control group.


Subject(s)
Aggregatibacter actinomycetemcomitans/isolation & purification , Orthodontic Appliances , Adolescent , Adult , Case-Control Studies , Child , Colony Count, Microbial , Cross-Sectional Studies , Dental Plaque/microbiology , Dental Plaque/pathology , Dental Plaque Index , Female , Gingival Hemorrhage/microbiology , Gingival Hemorrhage/pathology , Humans , Male , Orthodontic Appliances/classification , Orthodontic Brackets , Periodontal Index , Time Factors
7.
J Clin Periodontol ; 22(7): 578-83, 1995 Jul.
Article in English | MEDLINE | ID: mdl-7560242

ABSTRACT

Extensive data demonstrate that polymorphonuclear leukocytes (PMN) are the predominant cell type involved in periodontal disease and that gingival crevicular fluid constituents are influenced by the inflamed gingiva. The aim of the present study was to evaluate the ability of gingival crevicular washing (GCW) (a dilution of gingival crevicular fluid) from periodontal sites in different clinical conditions of modulating the PMN membrane receptors involved in motility, adhesion and phagocytosis before and after periodontal treatment. 10 patients affected by adult periodontitis (AP) were selected. From each patient, 2 test sites (TS) were chosen on the basis of a probing depth > 5 mm and attachment loss, and 2 control sites (CS) with probing depth < 3 mm without. Modifications of membrane receptor density of PMN from healthy donors incubated with GCW harvested from TS and CS was evaluated using fluorescent probes and flow cytometry. Compared to CS-GCW, TS-GCW before therapy increased the expression of the beta 2 integrin CD11b and the chemotactic receptor for the oligopeptide N-formyl methionyl leucyl phenylalanine (FMLP-R) while it reduced the expression of L-selectin. GCW collected from the same TS after the successful completion of periodontal treatment did not influence PMN receptors, indicating that the clinical improvement paralleled the disappearance of the PMN modulating capability contained in TS-GCW before therapy. In conclusion, the present data illustrate the relevant modifications occurring at PMN membrane in chronic adult periodontitis exerted by GCW obtained by a simple fluid collection technique. Thus, monitoring gingival crevicular fluid PMN activating capability may help disclose the presence of chronic periodontitis and may be useful in assessing successful treatment.


Subject(s)
Gingival Crevicular Fluid , Neutrophils/physiology , Periodontitis/therapy , Receptors, Cell Surface/genetics , Adult , CD18 Antigens/genetics , Cell Adhesion , Cell Movement , Female , Flow Cytometry , Fluorescent Dyes , Gene Expression , Gingival Crevicular Fluid/cytology , Humans , L-Selectin/genetics , Male , Middle Aged , N-Formylmethionine Leucyl-Phenylalanine/metabolism , Neutrophil Activation/genetics , Periodontal Attachment Loss/pathology , Periodontal Attachment Loss/therapy , Periodontal Pocket/pathology , Periodontal Pocket/therapy , Periodontitis/pathology , Phagocytosis , Receptors, Cell Surface/analysis , Receptors, Formyl Peptide , Receptors, Immunologic/genetics , Receptors, Peptide/genetics , Therapeutic Irrigation
8.
J Clin Periodontol ; 19(9 Pt 2): 715-22, 1992 Oct.
Article in English | MEDLINE | ID: mdl-1332988

ABSTRACT

The aim of the study was to compare the clinical and microbiological effects of topical application of a metronidazole gel and a single session of subgingival scaling in the treatment of adult periodontitis. An open, randomized controlled clinical study design was employed. Each of 24 subjects received the 2 treatments simultaneously each in 2 randomly selected quadrants of the dentition. The metronidazole, 25% gel was applied subgingivally on days 0 and 7. Scaling was carried out in one quadrant on day 0 and in one quadrant on day 7. Clinical and microbiological examinations were carried out before treatment and on days 21, 49, 91, 133, and 175 of the experimental period. The microbiological analyses included determination of total anaerobically cultivable bacteria, and relative proportions of anaerobes, aerobes, black-pigmented anaerobic Gram negative rods, Actinobacillus actinomycetemcomitans, streptococci, and spirochetes. Both treatments were effective in reducing probing pocket depth and bleeding on probing. Metronidazole tended to be a little better than scaling during the study period and the clinical effects of both treatments persisted during the whole 6 months observation period. Local metronidazole treatment induced a significant and long-lasting shift in the subgingival flora towards a composition more compatible with health and comparable to that obtained by mechanical debridement. Proportions of black pigmented anaerobes including Prevotella intermedia, and the number of spirochetes were significantly reduced after both treatments with a concomitant increase in the proportions of streptococci. While scaling resulted in a statistically significant increase in the proportion of A. actinomycetemcomitans, this was avoided after metronidazole treatment.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Bacteria/isolation & purification , Dental Scaling , Metronidazole/therapeutic use , Periodontitis/drug therapy , Periodontitis/therapy , Adult , Aged , Aggregatibacter actinomycetemcomitans/drug effects , Aggregatibacter actinomycetemcomitans/isolation & purification , Bacteria/drug effects , Bacteroides/drug effects , Bacteroides/isolation & purification , Delayed-Action Preparations , Dental Plaque/microbiology , Drug Implants , Female , Follow-Up Studies , Gels , Gingival Hemorrhage/drug therapy , Gingival Hemorrhage/microbiology , Gingival Hemorrhage/therapy , Humans , Male , Metronidazole/administration & dosage , Middle Aged , Periodontal Pocket/drug therapy , Periodontal Pocket/microbiology , Periodontal Pocket/therapy , Periodontitis/microbiology , Porphyromonas gingivalis/drug effects , Porphyromonas gingivalis/isolation & purification , Streptococcus/drug effects , Streptococcus/isolation & purification
9.
J Clin Periodontol ; 18(8): 598-604, 1991 Sep.
Article in English | MEDLINE | ID: mdl-1795057

ABSTRACT

The purpose of this study was to evaluate, on a short-term basis, the clinical and microbiological effects of a single course of scaling and root planing as compared with those obtained by flap surgery in patients with moderate to advanced periodontitis. 11 patients participated in the study. Using a split-mouth design, one quadrant of the mouth was treated with reverse bevel flap surgery, whereas the contralateral one was subjected to a single course of scaling and root planing. 2 approximal sites on single-rooted teeth with a pocket depth greater than or equal to 5 mm were monitored clinically and microbiologically for 16 weeks after active treatment. Both techniques resulted in a gain of probable attachment levels, a reduction in bleeding on probing and a reduced mean pocket depth, although 31.2% of the sites in the scaling and root planing group still had 6-7 mm deep pockets at 8 and 16 weeks after treatment. Both techniques reduced median relative proportions and frequencies of detection of black-pigmented Bacteroides species. A highly statistically significant increase (p less than 0.01) in median proportions of oral streptococci was recorded only for surgery within the 1st month post-operatively. No correlation was found between residual pocket depth and any of the microbiological parameters considered in the study, suggesting that residual pocket depth does not exert a significant influence on bacterial subgingival recolonization after therapy. The results from this study suggest that surgery can be as effective as scaling and root planing in favoring the establishment of micro-organisms compatible with periodontal health, although this effect is limited to the 1st month after therapy.


Subject(s)
Bacteria/isolation & purification , Dental Scaling , Periodontal Pocket/microbiology , Periodontitis/surgery , Periodontitis/therapy , Root Planing , Surgical Flaps , Adult , Aged , Aggregatibacter actinomycetemcomitans/isolation & purification , Bacteria, Anaerobic/isolation & purification , Bacteroides/isolation & purification , Dental Plaque/microbiology , Dental Plaque/pathology , Gingival Hemorrhage/pathology , Humans , Middle Aged , Oral Hygiene , Patient Education as Topic , Periodontal Pocket/pathology , Periodontal Pocket/surgery , Periodontal Pocket/therapy , Periodontitis/microbiology , Streptococcus/isolation & purification
10.
Oral Microbiol Immunol ; 6(3): 129-33, 1991 Jun.
Article in English | MEDLINE | ID: mdl-1945494

ABSTRACT

Recently published taxonomic studies of viridans streptococci have resulted in several changes in the nomenclature and definition of oral streptococcal species. With this background, the ecology of streptococci in the oropharyngeal cavities was reinvestigated. The results based on the examination of 1426 streptococcal isolates confirmed and extended earlier findings. Apart from mature supragingival plaque, which contained a mixture of all orally encountered streptococci, each site showed a characteristic streptococcal flora. Initial dental plaque formation is primarily associated with Streptococcus sanguis, Streptococcus mitis biovar 1 and Streptococcus oralis. Our investigation showed that S. sanguis and S. mitis biovar 1 were the most prominent streptococci, also on buccal mucosa. In contrast, S. oralis was almost exclusively found in initial dental plaque. Streptococcus gordonii, formerly part of S. sanguis, was found in small numbers on the oropharyngeal mucosa and in mature supragingival plaque. The dorsum of the tongue was dominated by S. mitis biovar 2 and Streptococcus salivarius, the latter of which was predominant also on the pharyngeal mucosa. Streptococcus anginosus was by far the most predominant streptococcus in subgingival plaque. Immunoglobulin A1 (IgA1) protease-producing streptococci were primarily isolated from initial dental plaque and from the buccal mucosa. This lends further support to the concept of IgA1 proteases being important for the ability of streptococci to evade the local immune defence during their initial colonization of certain oral surfaces.


Subject(s)
Dental Plaque/microbiology , Pharynx/microbiology , Streptococcus/isolation & purification , Adult , Dental Plaque/immunology , Ecology , Humans , Immunoglobulin A/immunology , Immunoglobulin A, Secretory/immunology , Mouth Mucosa/microbiology , Streptococcus/enzymology , Streptococcus/immunology , Tongue/microbiology
SELECTION OF CITATIONS
SEARCH DETAIL
...