The influence of parenterally administered alpha-tocopheryl acetate to sows on the vitamin E status of the sows and suckling piglets and piglets after weaning.

The main aim of the study was to test if parenteral administration of alpha-tocopheryl acetate twice before farrowing and weaning could increase the vitamin E status of the newborn piglets and piglets after weaning. In Trial I eight sows were given 1.5 g alpha-tocopheryl acetate intramuscularly 7 and 2 days before farrowing. Eight sows were untreated controls. The experimental sows had a higher vitamin E concentration in colostrum than the controls. No significant difference between the groups existed in milk at weaning. The serum vitamin E concentration in the experimental piglets 2 and 5 days after farrowing was higher than in the controls. Fifteen days after farrowing the difference between the groups had nearly disappeared. The serum vitamin E concentration in the piglets in the control group was higher during the first days after farrowing than later, and was gradually reduced until at least 2 weeks after weaning. In Trial II, eight sows were given 1.5 g of alpha-tocopheryl acetate 7 and 2 days before weaning of their piglets. They had higher vitamin E concentrations in milk and serum than untreated control sows at weaning. The increase did not, however, influence the serum vitamin E concentration of the piglets. The lowest concentration of vitamin E in serum of the piglets was reached at 45 days after farrowing. The activity of the selenium-dependent enzyme glutathione peroxidase in the serum of piglets was very low during the first week of life in both groups despite the fact that the sows' feed had been supplemented with 0.35 mg selenium/kg. This indicates that the selenium status of newborn piglets might be more critical for their health than their vitamin E status.

The influence of acidic diets on the acid-base balance of dry cows and the effect of fertilization on the mineral content of grass.

To investigate the safety and practicality of an acidic concentrate in milk fever prevention, the pH, carbon dioxide, standard bicarbonate, and base excess of whole blood and the pH in the urine were measured in three treatment groups of dry cows after 14 and 21 d of feeding an acidogenic diet (experiment 1). The dietary cation-anion differences (DCAD) of cows on treatments 1 (n = 11), 2 (n = 13), and 3 (n = 12) were +2275, -262, and -1185 meq/d, respectively. No changes in any parameters were found from the beginning to the end of the experiment in cows on treatment 1. In cows on treatment 2, a significant reduction in urine pH was observed, and in cows on treatment 3 significant decreases in all parameters except blood pH were observed. Mineral analyses of grass samples from fields fertilized with N from NH4NO3, Ca(NO3)2, or (NH4)2SO4, with S from (NH4)2SO4, and with different amounts of K from KCl or K2SO4 and of Cl from KCl revealed DCAD ranging from -14 to +726 meq/kg of dry matter (experiment 2). Fertilization with Cl increased the chloride concentration in the crop and had the largest effect on DCAD. The results indicate that the use of acidic concentrates is not a health hazard for dry cows, at least not when the DCAD is greater than about -1200 meq/d or about -140 meq/kg of dry matter.

Effect of selenate as a feed supplement to dairy cows in comparison to selenite and selenium yeast.

The main aim of this trial was to define the possible differences between selenite and selenate in their ability to increase the selenium (Se) concentration of milk, in comparison with organic Se. Dairy cows (n = 42) were fed a basal diet containing .10 to .12 mg Se/kg DM for 5 mo and were then divided into four groups of 10 or 11, as similar as possible in age and stage of lactation. During the next 84 d, the cows in three of the groups were supplemented with 3 mg of Se daily, whereas the cows in one control group remained unsupplemented. The Se supplement was given as sodium selenite, sodium selenate, or a Se yeast product. The total Se concentration of the diets varied with the cows' stage of lactation and was for the supplemented groups .24 to .31 mg/kg DM, but remained between .10 and .12 mg/kg in the control group. At the end of the trial, the mean whole blood Se concentrations in the selenite, selenate, yeast, and control groups were 138, 141, 165, and 104 microg/L, respectively. The Se concentration in plasma apparently reached a plateau level within 4 wk, at approximately 75 microg/L in the selenite group, 80 microg/L in the selenate group, and 90 microg/L in the yeast group. In the control group the mean concentration in plasma remained at approximately 50 microg/L. The increase of the activity of glutathione peroxidase (GSH-Px) in the erythrocytes was significantly higher in the supplemented groups than in the control group. The mean concentrations of Se in milk in the selenite, selenate, and yeast groups were 16.4, 16.4, and 31.2 microg/L, respectively, whereas the concentration remained at approximately 14 microg/L in the control group. The milk Se concentration reached a plateau within 1 wk after the start of Se supplementation. Dietary supplementation with selenite and selenate, thus, had only a limited effect on the Se concentration in milk, and there was no significant difference between the two inorganic compounds in any variable measured. Organic Se was much more effective than inorganic Se in increasing the concentration of Se in milk.

The influence of dietary selenium as selenium yeast or sodium selenite on the concentration of selenium in the milk of Suckler cows and on the selenium status of their calves.

The aim of this trial was to determine whether the selenium status of suckling calves could be improved by supplementing their dams' diet with organic Se instead of sodium selenite. A herd of 103 Hereford cows, which were on grass paddocks all year round, was divided into two groups. Both groups had free access to a mineral supplement that contained 30 mg of Se/kg; for one group the source of the Se was a Se yeast product, and for the other group the source was sodium selenite. The basal feed contained .02 mg of Se/kg DM. During the trial, the mean daily consumption of the mineral supplement was approximately 110 g/cow. The calving season started in the middle of March and ended in the middle of May. Blood samples were taken from 11 cows and their calves in the yeast group and from nine in the selenite group at the end of April and again at the beginning of June, and milk samples were taken at the same times. At both samplings, the concentration of Se in whole blood and the activity of glutathione peroxidase (GSH-Px) in the erythrocytes of the cows and calves in the yeast group were higher than in the samples from the animals in the selenite group. The same pattern was seen for plasma, except for the cows at the first sampling. The mean concentrations of Se in whole blood from calves in the yeast and selenite groups were 130 and 84 microg/L, respectively, and plasma concentrations were 48 and 34 microg/ L, respectively. Mean Se concentration in the milk from the yeast group (17.3 microg/L) was higher than that in milk from the selenite group (12.7 microg/L). There were significant correlations (r = .59 to .68) between the concentrations of Se in the cow's milk or cow's whole blood compared with Se concentrations in the calves whole blood and plasma or with the erythrocyte GSH-Px activity of the calves. The Se status of the calves in the selenite group was considered to be marginal, but the status of the calves in the yeast group was considered to be adequate. Supplementation of the suckler cows' diet with organic Se in the form of Se yeast rather than sodium selenite improved the Se status of their calves when the Se was mixed into a mineral supplement containing 30 mg of Se/kg. In practice, such supplementation would probably eliminate the risk of nutritional muscular degeneration in suckling calves.

Selenite and selenium yeast as feed supplements to growing fattening pigs.

Twenty-four cross-bred fattening pigs weighing, on average, 24 kg were divided into three equal groups and fed a basic diet containing 0.1 mg selenium/kg. The diet of group A was supplemented with 0.3 mg selenium/kg derived from selenium yeast, group B with 0.1 mg selenium/kg from the same source, and group C with 0.3 mg/kg from sodium selenite. The pigs were slaughtered after 103 days. There was no significant difference between the mean whole blood selenium concentration of the pigs supplemented with selenium yeast neither after five nor after nine weeks of supplementation (group A, 201 and 213 micrograms/litre; group B 192 and 201 micrograms/litre, respectively), but the group supplemented with selenite had significantly lower concentrations both after five and nine weeks (158 and 159 micrograms/litre, respectively). There were no significant differences between the mean activity of glutathione peroxidase (GSH-Px; in the whole blood of the three groups (group A, 348 and 327 mukat*/litre, group B, 342 and 302 mukat/litre; group C, 332 and 300 mukat/litre, after five and nine weeks, respectively). The concentration of selenium in the liver of the pigs supplemented with organic selenium was significantly higher than in the pigs supplemented with selenite (group A, 0.54 mg/kg wet weight; group B, 0.54 mg/kg; group C, 0.40 mg/kg). No deposits of lipofuscin or ceroid pigments were observed in the histological preparations of liver and heart from any of the pigs.

A comparative study of the effectiveness of calcium propionate and calcium chloride for the prevention of parturient paresis in dairy cows.

The efficacy of calcium propionate for the prevention of parturient paresis (milk fever) was compared with that of calcium chloride using 194 cows that had experienced milk fever during the previous calving. The cows were mainly of the Swedish Red and White and Swedish Friesian breeds and were divided randomly into an experimental group (n = 99) and a control group (n = 95). The cows in the experimental group received up to six boluses of 20 g of calcium as calcium propionate between 36 h before and 24 h after calving; the cows in the control group received up to four doses of 54 g of calcium as a commercially available oily solution of calcium chloride during the same period. Incidence of milk fever was recorded as the percentage of cows that were treated by a veterinarian because they showed clinical signs of the disease and had a blood calcium concentration less than 8.0 mg/dl. Twenty-five (25.3%) cows in the experimental group and 22 cows (23.2%) in the control group developed milk fever. The incidence of milk fever for cows in both groups was significantly lower than the 36.0% found in 713 cows that had experienced milk fever during their previous calving but received no prophylactic treatment. Therefore, calcium propionate was considered to have had a significant preventive effect, comparable with that of calcium chloride.

Effects of chromium supplementation on the growth and carcass quality of bulls fed a grain-based diet during the finishing period.

Two groups of 12 young bulls, as similar as possible with respect to age and weight, were fed a basic diet of hay and concentrates ad libitum for 170 days. The concentrate fed to one of the groups was supplemented with 0.20 mg of organic chromium per kg DM from a fodder yeast product so that the concentration of chromium in the total dry matter of their diet was 0.90 mg/kg, compared with 0.72 mg/kg in the diet of the control group. No significant differences were observed between the two groups of animals in terms of either their daily weight gain or any of the parameters of carcass quality examined. It is therefore concluded that in Sweden there is no reason to add chromium routinely to the diet of intensively fed, growing bulls.

Selenite and selenium yeast as feed supplements for dairy cows.

The availability of inorganic and organic forms of selenium to dairy cows was studied by giving 25 cows supplementary selenium for 9 months either as sodium selenite or as a selenium-containing yeast product. Group I (eight cows) received 3.0 mg selenium as sodium selenite daily, group II (nine cows) received 3.0 mg selenium as the selenium yeast product, and group III (eight cows) received 0.75 mg selenium as the selenium yeast product. The total selenium contents of the ration were 0.26-0.32 mg/kg feed dry matter for groups I and II, and 0.16-0.18 mg/kg for group III. The supplement of 0.75 mg selenium daily from the yeast product maintained the selenium concentrations of whole blood and milk at the same levels as 3.0 mg selenium as sodium selenite, and 3.0 mg selenium from the yeast product increased the selenium concentration of whole blood by approximately equal to 40% and that of milk by approximately equal to 100%. The activity of glutathione peroxidase in erythrocytes of the group given selenite was not significantly different from that in either of the groups given the yeast product. The concentrations of selenium in the tissues of two cows from each group were marginal to adequate, and there was a trend for the concentrations to be higher in the tissues of the cows supplemented with the yeast product.

Variations with breed, age, season, yield, stage of lactation and herd in the concentration of urea in bulk milk and individual cow's milk.

The concentration of urea in the milk of 510 dairy cows in 10 herds was determined at regular intervals for a year. The herds contained approximately equal numbers of Swedish Red and White, and Swedish Holstein cows. The mean +/- sd concentration in the samples from individual cows was 5.32 +/- 1.13 mmol/l, and the mean concentration in bulk milk was 5.39 +/- 0.96 mmol/l. These values indicated that on average the herds were fed too much protein relative to their intake of energy throughout the year. Herd factors had a strong influence on the milk urea concentration. The concentration was lower during the first month of lactation than later in the lactation, and lower when the cows were housed during the winter than when they were grazing. There was a weak positive relationship between the daily milk yield and urea concentration, particularly during late lactation, but there was no relationship with either breed or age. Bulk milk urea was a reliable guide to the average urea concentration of a herd.

Eimeria alabamensis infection as a cause of diarrhoea in calves at pasture.

Large numbers of oocysts of Eimeria alabamensis have been found in the faeces of calves suffering from diarrhoea shortly after being turned out to pasture. To investigate the source and clinical significance of this coccidial infection, the numbers of oocysts excreted, the consistency of the faeces and the growth rates of four groups of 12 calves were compared. Group I calves were kept indoors and their diet was unchanged, Group II calves were turned out onto a previously ungrazed pasture, Group III calves were turned out onto a permanent pasture and Group IV calves were kept indoors and fed cut grass from a previously ungrazed field. Eight days after the animals were turned out there was an almost 1000-fold increase in the numbers of oocysts in the faeces of Group III calves, the dominant species being E. alabamensis, but there were only minor fluctuations in the numbers of oocysts excreted by the other groups. It was therefore concluded that the source of the infection was oocysts that had overwintered on the permanent pasture. Most of the calves in Group III developed watery diarrhoea 5 days after turnout, but there was only a slight softening of the faeces of the calves in Groups II and IV at about the same time. The faeces of the calves in Group I was of firm consistency throughout the trial. The calves in Group III lost 18 kg during the 24 day period following turnout, whereas the calves in the other groups gained between 6 and 18 kg.

The influence of the dietary balance between energy and protein on milk urea concentration. Experimental trials assessed by two different protein evaluation systems.

Twenty-three dairy cows were fed rations with different proportions of energy and digestible crude protein (DCP). When the ration was balanced for energy and DCP according to Swedish standard the cows' milk urea concentration was 4.66-4.92 mmol/l (95% CI of mean). With increasing intakes of DCP, fed together with standard levels of energy, the mean milk urea concentration increased in proportion to the surplus of DCP. In contrast, the concentration of urea decreased when the cows were overfed with energy at the same time as they were underfed with protein. When the rations were recalculated in accordance with the AAT/PBV system for dietary protein evaluation the 95% CI for the mean milk urea concentration of the cows receiving a balanced ration was 3.76-4.56 mmol/l. The concentration of urea was dependent primarily on the PBV. When the 2 protein evaluation systems were compared there was a strong correlation between PBV and DCP. Ammonia was the only constituent of the rumen whose concentration was strongly correlated with the milk urea concentration. Taken together with earlier data the present results suggest that a milk urea concentration between 4.0 and 5.5 mmol/l should be regarded as normal at least when cows are fed conventional feedstuffs.

The relationships between seasonal variations in the concentration of urea in bulk milk and the production and fertility of dairy herds.

The concentration of urea was measured in bulk milk samples taken on seven occasions during a period of 13 months from 1,658-1,815 of the 2,100 suppliers of milk to a dairy. The mean concentration of urea was significantly higher when the cows were grazing than when they were housed, but there were no significant variations within either of these periods. In herds with very low milk urea concentrations while they were housed (< 2.4 mmol/l) the mean increase during the grazing season was approximately 2.0 mmol/l, whereas in herds with very high urea concentrations while they were housed (> 6.0 mmol/l) there was a slight decrease in urea concentration during the grazing season. The annual milk yield of herds with low urea concentrations was significantly lower than the yield of herds with intermediate or high concentrations. The interval between calving and first insemination was significantly longer in herds with low milk urea concentrations, but these herds also had a higher 56-day non-return rate.

Excretion of Eimeria oocysts in calves during their first three weeks after turn-out to pasture.

The numbers of Eimeria oocysts per gram (opg) and the dry matter content of 449 faecal samples taken from 54 calves in 8 herds in south west Sweden were determined during the last 2 weeks before and the first 3 weeks after the animals were turned out to pasture. While they were housed only between 0 and 580 opg were found and in 2 of the herds the numbers of oocysts remained low after turn-out. In the other 6 herds the numbers of oocysts increased after 8 to 10 days and reached a peak of between 1080 and 80,803 opg 9 to 18 days after turn-out. By 21 to 24 days after turn-out the opg-values had declined to their initial levels. Eimeria alabamensis accounted for most of the increase, but small numbers of oocysts of E. auburnensis, E. bovis, E. bukidnonensis, E. cylindrica, E. ellipsoidalis, E. pellita, E. subspherica, E. wyomingensis and E. zuernii were also observed. The interval between turn-out and the start of the increase in excretion of oocysts corresponded closely to the prepatent period of E. alabamensis and overwintered oocysts were therefore the most likely source of the infection. In 6 of the herds the dry matter content of the faeces of the calves decreased after turn-out and 56% of the calves had clinical diarrhoea. Although it cannot be excluded that change of diet may have contributed to these symptoms, E. alabamensis infection is suggested as a potential cause of diarrhoea and loss of condition in calves in Sweden during their first weeks on pasture.

The effect of additional feeding on the fertility of high-yielding dairy cows.

One hundred and ninety three pairs of cows were selected from 38 herds, and the numbers of each pair were assigned at random to experimental and control groups. Each control cow was fed according to its yield with the feedstuffs normally used by the herd. Each experimental cow was fed in the same way and, in addition, received 500 g of a feed additive twice daily from the 40th day of lactation until first insemination or to the 75th day of lactation if no visible oestrus had been observed between days 40 and 75. One kg of the feed additive contained glucogenic substances (glycerol and Ca-propionate) equivalent to 1.90 moles of glucose, 13.6 MJ metabolizable energy, and 180 g digestible crude protein. There were no significant differences between the groups with respect to their mean daily milk yield, their plasma glucose concentrations at first insemination, or the interval between calving and first insemination. However, the experimental cows had a significantly shorter interval between calving and last insemination (mean difference 11.0 days). The pregnancy rate was significantly higher among cows with high than among cows with low plasma glucose concentrations. There was also a tendency towards a reduced pregnancy rate among cows with either high (greater than 7.0 mmol/litre) or low (less than 4.0 mmol/litre) milk urea concentrations.

The relationships between the fertility of dairy cows and clinical and biochemical measurements, with special reference to plasma glucose and milk acetone.

Blood and milk samples were taken at first insemination in 352 dairy cows from 18 herds for charting the relation between clinico-chemical parameters and fertility rate. Neither total protein, albumin, globulin, AST, bilirubin, bile acids, calcium, magnesium and phosphorus in blood nor urea in milk was significantly related to the rate of pregnancy. On the other hand, there was a significant difference for plasma glucose between cows that became pregnant and those that did not. Cows with low plasma glucose concentrations at first insemination also had low values four and seven weeks after calving, indicating that it is primarily cows with chronically low blood glucose which are likely to have reduced fertility. At first insemination there was no increase in the concentration of acetone in milk of cows with reduced fertility. However, they had had increased milk acetone concentrations three to five weeks after calving. It therefore seems possible to evaluate the risk of reduced fertility by measuring either blood glucose or milk acetone some weeks after calving. Owing to the difficulties associated with the sampling and laboratory techniques for glucose, analyses of milk acetone are more suitable under field conditions.

Effect of vitamin E supplementation on weight gain, immune competence, and disease incidence in barley-fed beef cattle.

The aim of the study was to investigate whether vitamin E supplements in larger amounts than recommended could reduce incidence of disease, improve immune competence, and increase rate of weight gain of conventionally barley-fed beef cattle. Mean daily intake of vitamin E by individual calves in the experimental group was 200 mg during the first 2 mo, 400 mg during the next 2 mo, and 600 mg during the rest of the period. Corresponding daily intakes of vitamin E for the control group were 50, 100, and 150 mg. Mean plasma vitamin E of the experimental group increased from .49 mg/L at the start of the trial to 2.03 mg/L at the end, but that of the control group was lower at the end (.36 mg/L) than at the beginning (.53 mg/L). No significant differences were observed between the groups concerning incidence of disease or magnitude of lymphocyte stimulation. The results indicated that there was a surprisingly poor biological availability of the dietary vitamin. Therefore, a comparison in reality was made between calves with inadequate and normal vitamin E status. The differences in daily BW gain and time to reach slaughter weight thus probably were effects of the low vitamin E status rather than positive effects of additional vitamin in the diet.

Vitamin E status in newborn lambs with special reference to the effect of dl-alpha-tocopheryl acetate supplementation in late gestation.

Pregnant ewes were supplemented with dl-alpha-tocopheryl acetate, either as a single intramuscular dose (500 mg two weeks before lambing) or perorally (150 mg daily during 3-4 weeks before lambing). Ewes without such a supplementation were controls. The vitamin E supplemented ewes had nearly twice as high vitamin E (alpha-tocopherol) concentrations as the unsupplemented control ewes at lambing both in serum and in colostrum. The vitamin E concentration in colostrum was 5-11 higher than in milk 1 week after lambing. Both supplementations somewhat increased the vitamin E serum concentration of the newborn lambs, but the increase was negligible in comparison with the effect produced by the consumption of colostrum. All lambs had very low serum concentrations at birth. The lambs from the supplemented ewes had significantly higher serum values than the control lambs 24 h after birth. The ewes had somewhat higher selenium status at birth than their offsprings when evaluated by glutathione peroxidase (GSH-Px) in the erythrocytes. It seems reasonable that nutritional muscular degeneration may arise in newborn lambs with a normal selenium status if their vitamin E status is critical, either because of an inadequate consumption of colostrum or because of a vitamin E deficient diet during pregnancy with a low vitamin concentration of colostrum as a consequence.