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1.
Ann R Coll Surg Engl ; 2024 Feb 16.
Article in English | MEDLINE | ID: mdl-38362753

ABSTRACT

INTRODUCTION: The utilisation of laparoscopic appendicectomy (LA) in children remains contentious despite the well-recognised advantages of laparoscopic surgery. The purpose of this study was to compare intraoperative and postoperative outcomes in LA and open appendicectomy (OA) when performed by adult general surgeons outside specialist paediatric practice in younger children. METHODS: A retrospective review of all patients under the age of 13 who underwent LA for suspected appendicitis over a two-year period was conducted. These were case-matched with an equivalent number of patients who underwent OA during the same period. Intraoperative and postoperative outcomes were compared. RESULTS: Fifty-one patients underwent LA during the study period. Patient demographics were statistically equivalent with the OA cohort. A statistically significant longer median operating time (58 vs 49min) was noted in the LA group, but intraoperative outcomes were otherwise comparable. LA, when compared with OA, was associated with a significant improvement in postoperative length of stay (2 vs 3 days, p < 0.001), postoperative complication rate (0% vs 6%, p = 0.01), negative appendicectomy rate (3.9% vs 17.6%, p < 0.001) and 30-day readmission rate (0% vs 5.9%, p = 0.03). No patients in the LA group required conversion to open surgery. CONCLUSION: LA can be safely delivered by adult general surgeons to younger paediatric populations outside the setting of paediatric specialist practice, with statistically significant improvements in postoperative outcomes noted when compared with OA. These findings are of importance in the current healthcare context where adult general surgeons continue to perform the majority of paediatric appendicectomies.

2.
Br J Surg ; 108(2): 128-137, 2021 03 12.
Article in English | MEDLINE | ID: mdl-33711141

ABSTRACT

BACKGROUND: Mixed results are reported on clinical and cancer outcomes in laparoscopic rectal cancer surgery (LRCS) compared with robotic rectal cancer surgery (RRCS). However, more favourable functional outcomes are reported following RRCS. This study compared urinary and sexual function following RRCS and LRCS in male and female patients. METHODS: A systematic review and meta-analysis of urinary and sexual function after RRCS and LRCS was performed following PRISMA and MOOSE guidelines, and registered prospectively with PROSPERO (ID:CRD42020164285). The functional outcome reporting tools most commonly included: the International Prostate Symptom Score (IPSS), International Index of Erectile Function (IIEF) and Female Sexual Function Index (FSFI). Mean scores and changes in mean scores from baseline were analysed using RevMan version 5.3. RESULTS: Ten studies were included reporting on 1286 patients. Some 672 patients underwent LRCS, of whom 380 (56.5 per cent) were men and 116 (17.3 per cent) were women (gender not specified in 176 patients, 26.2 per cent). A total of 614 patients underwent RRCS, of whom 356 (58.0 per cent) were men and 83 (13.5 per cent) were women (gender not specified in 175 patients, 28.5 per cent). Regarding urinary function in men at 6 months after surgery, IPSS scores were significantly better in the RRCS group than in the LRCS group (mean difference (MD) -1.36, 95 per cent c.i. -2.31 to -0.40; P = 0.005), a trend that persisted at 12 months (MD -1.08, -1.85 to -0.30; P = 0.007). ΔIIEF scores significantly favoured RRCS at 6 months [MD -3.11 (95%CI -5.77, -0.44) P <0.021] and 12 months [MD -2.76 (95%CI -3.63, -1.88) P <0.001] post-operatively. Mixed urinary and sexual function outcomes were reported for women. CONCLUSION: This meta-analysis identified more favourable urinary and erectile function in men who undergo robotic compared with conventional laparoscopic surgery for rectal cancer. Outcomes in women did not identify a consistently more favourable outcome in either group. As robotic rectal cancer surgery may offer more favourable functional outcomes it should be considered and discussed with patients.


Subject(s)
Female Urogenital Diseases/etiology , Laparoscopy/adverse effects , Male Urogenital Diseases/etiology , Rectal Neoplasms/surgery , Robotic Surgical Procedures/adverse effects , Erectile Dysfunction/etiology , Female , Humans , Laparoscopy/methods , Male , Robotic Surgical Procedures/methods , Urination Disorders/etiology
3.
BJS Open ; 4(6): 1256-1265, 2020 Dec 02.
Article in English | MEDLINE | ID: mdl-33047514

ABSTRACT

BACKGROUND: Improved diagnostic biomarkers are required for acute appendicitis. The circulating fibrocyte percentage (CFP) is increased in inflammatory states, but has not been studied in acute appendicitis. This study aimed to determine CFP in acute appendicitis and compare diagnostic accuracy with standard serological biomarkers. METHODS: A prospective cohort study was carried out between June 2015 and February 2016 at University Hospital Limerick. The CFP was determined by dual-staining peripheral venous samples for CD45 and collagen I using fluorescence-activated cell sorting, and correlated with histopathological diagnoses. The accuracy of CFP in determining histological acute appendicitis was characterized and compared with the white cell count, C-reactive protein concentration, neutrophil count, lymphocyte count and neutrophil : lymphocyte ratio. RESULTS: Of 95 adults recruited, 15 were healthy individuals and 80 had suspected appendicitis at presentation. Forty-six of these 80 patients had an appendicectomy, of whom 34 had histologically confirmed appendicitis. The CFP was statistically higher in patients with pathologically proven acute appendicitis than in healthy controls (median 6·1 (i.q.r. 1·6-11·6) versus 2·3 (0·9-3·4) per cent respectively; P = 0·008). The diagnostic accuracy of CFP, as determined using the area under the receiver operating characteristic (ROC) curve, was similar to that of standard biomarkers. In multinomial regression analysis, only raised CFP was retained as an independent prognostic determinant of acute appendicitis (odds ratio 1·57, 95 per cent c.i. 1·05 to 2·33; P = 0·027). CONCLUSION: The CFP is increased in histologically confirmed acute appendicitis and is as accurate as standard serological biomarkers in terms of diagnosis.

5.
Tech Coloproctol ; 24(7): 747-755, 2020 07.
Article in English | MEDLINE | ID: mdl-32394102

ABSTRACT

Minimally invasive laparoscopic low or ultra-low anterior resection may present as a complex, technically difficult challenge to even the most experienced of colorectal surgeons. This is because, within the narrow confines of the pelvis, there is usually limited visibility, and difficult manoeuvrability of rigid laparoscopic instrumentation with resulting poor access. The utilisation of robotic technology makes sense within the narrow confines of the pelvis. Several studies including recent meta-analyses of randomized controlled trials and propensity-score-matched cohorts have shown reduced rates of conversion to open. Some studies have also shown benefits including improved short-term outcomes and oncological benefits. However, robotic ultra-low anterior resection has a steep learning curve and many of the benefits of robotic surgery have not been fully realised, because the majority of surgeons are in the early phase of the learning curve. This 'How I do It' article provides a detailed description of the important technical points that may help in maximising success in performing robotically assisted laparoscopic ultra-low anterior resection.


Subject(s)
Laparoscopy , Proctectomy , Rectal Neoplasms , Robotic Surgical Procedures , Robotics , Humans , Rectal Neoplasms/surgery
6.
Tech Coloproctol ; 21(11): 863-868, 2017 Nov.
Article in English | MEDLINE | ID: mdl-29149428

ABSTRACT

BACKGROUND: Ileostomy reversal is associated with surgical site infection (SSI) rates as high as 37%. Recent literature suggests that employing a purse-string approximation (PSA) of the reversal wound reduces this rate of SSI. Thus we wished to perform a randomised controlled trial to compare SSI rates in purse-string versus linear closure (PLC) wounds following ileostomy reversal. METHODS: A randomised, controlled trial was conducted at University Hospital Limerick. Sixty-one patients undergoing ileostomy reversal were included. Thirty-four patients were randomised to PSA and 27 patients to linear closure. The primary endpoint was incidence of SSI and secondary endpoints measured were quality of life and satisfaction with cosmesis. Statistical analysis was performed on a per protocol basis using SPSS version 22.0. RESULTS: Three patients in the PSA group developed an SSI compared to 8 in the PLC group at 30 days (8 vs 30%, p = 0.03). The mean time to SSI diagnosis was faster in the PSA group (3 vs 12.3 days, p = 0.08). Patients who developed SSI experienced a longer mean length of stay (6.8 vs 11.4 days, p = 0.012). On multivariate analysis, PLC was the only predictive factor of SSI formation (p < 0.001). There was no difference in patient satisfaction between the two study groups (p = 0.14). CONCLUSIONS: PSA of wounds following ileostomy reversal significantly reduces SSI formation compared to linear approximation without any effect on patient satisfaction.


Subject(s)
Ileostomy , Patient Satisfaction , Surgical Wound Infection/etiology , Suture Techniques/adverse effects , Aged , Female , Humans , Length of Stay , Male , Middle Aged , Quality of Life , Surgical Wound Infection/diagnosis , Time Factors
7.
Tech Coloproctol ; 21(9): 721-727, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28929257

ABSTRACT

BACKGROUND: Minimally invasive surgery is associated with several patient-related benefits, including reduced length of hospital stay and reduced blood loss. Robotic-assisted surgery offers many advantages when compared with standard laparoscopic procedures, including a stable three-dimensional binocular camera platform, motion smoothing and motion scaling, improved dexterity and ergonomics. There are limited data on the effectiveness of the dual-console DaVinci Xi platform for teaching resident surgeons. The goal of this study was to examine preliminary outcomes following the introduction of a dual-console robotic platform in our institution. METHODS: A retrospective review of our prospectively maintained patient database was performed. The first ten dual-console resident-performed procedures in colorectal surgery were compared with matched cases performed on a single console by the trainer. Patient demographics, operative times and patient outcomes were compared. RESULTS: Twenty patients were included in this study. There was no significant difference in console time (p = 0.46) or total operative time (p = 0.52) when residents and trainers were compared. Patient outcomes were equivalent, with no difference in length of stay, morbidity or mortality. CONCLUSIONS: The DaVinci Xi dual-console platform is a safe and effective platform for training junior surgeons. The dual-console system has the potential to alter surgical training pathways.


Subject(s)
Colorectal Surgery/education , Digestive System Surgical Procedures/education , Medical Staff, Hospital/education , Robotic Surgical Procedures/education , Adult , Aged , Aged, 80 and over , Databases, Factual , Female , Humans , Length of Stay , Male , Middle Aged , Operative Time , Prospective Studies , Retrospective Studies , Robotic Surgical Procedures/methods , Treatment Outcome
8.
Colorectal Dis ; 19(8): e312-e315, 2017 Aug.
Article in English | MEDLINE | ID: mdl-28649762

ABSTRACT

AIM: Abdominal salvage surgery for a failed ileal pouch-anal anastomosis (5) is safe and feasible in experienced hands. When salvaging an ileal pouch or creating a new J, S or W pouch may not be feasible, construction of an H-pouch may be the final option. This study reports a single colorectal surgeon's experience on H-pouch anal anastomosis in patients referred with a failed ileal pouch. METHOD: Patients undergoing transabdominal salvage surgery with H-pouch formation for a failed pouch from February 2012 to May 2016 were evaluated. RESULTS: Five patients were identified with a mean age of 46 (22-63) years. The pathological diagnosis was mucosal ulcerative colitis in all patients. Three patients had an initial traditional two-stage J-pouch creation and two patients had an initial three-stage approach. The median time to redo pouch surgery after the index IPAA creation was 99 (11-158) months. One patient required excision of the pouch and two patients had a complication within 30 days of surgery. CONCLUSION: The H-pouch is a good alternative for a failed IPAA when another type of reservoir is not an option.


Subject(s)
Colonic Pouches/adverse effects , Postoperative Complications/surgery , Proctocolectomy, Restorative/methods , Reoperation/methods , Salvage Therapy/methods , Adult , Colitis, Ulcerative/surgery , Feasibility Studies , Female , Humans , Male , Middle Aged , Postoperative Complications/etiology , Proctocolectomy, Restorative/adverse effects , Retrospective Studies , Treatment Failure , Young Adult
10.
Tech Coloproctol ; 18(7): 653-60, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24500724

ABSTRACT

BACKGROUND: The aim of the present study was to develop a unique anatomic replica of the mesocolon using digital graphical software in order to provide an educational template for mesosigmoidectomy. METHODS: The colon and mesocolon were fully mobilized from ileocecal to mesorectal levels in a cadaver. Both colon and mesocolon provided a template from which to generate a three dimensional replica in ZBrush. The model was deformed in ZBrush, to compare and contrast current and classic interpretations of mesosigmoidal topography. An animation was developed in which the replica was deformed to mimic operative mobilization. Contiguous shape changes were captured in two-and-a-half-dimensional (2.5D) screen snapshots. This was repeated for medial to lateral and lateral to medial mobilization of the mesosigmoid. RESULTS: Topographic differences between classic and current appraisals of mesocolic anatomy were evident in 2.5D format. Using the model generated, contiguous shape changes during mesosigmoidal mobilization (i.e., between the left mesocolon, mobile/apposed mesosigmoid, and mesorectum) were replicated in animation format. By extracting and compiling 2.5D screen grabs a pictorial chronology of mobilization was developed. CONCLUSIONS: Recent advances in mesocolic topography can be captured and rendered using advanced digital sculpting software with high-end graphics capabilities. This approach permits a depiction of contiguous changes in mesosigmoidal topography during mesosigmoidal mobilization. A compilation of images in either animation or screen grab format obviates the interpolation of shape changes required using standard educational approaches.


Subject(s)
Imaging, Three-Dimensional , Mesocolon/surgery , Software , Surgery, Computer-Assisted/methods , Cadaver , Computer Simulation , Humans , Models, Anatomic , Sensitivity and Specificity
11.
BJOG ; 120(10): 1240-7; discussion 1246, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23782995

ABSTRACT

OBJECTIVE: To compare early home biofeedback physiotherapy with pelvic floor exercises (PFEs) for the initial management of women sustaining a primary third-degree tear. DESIGN: Single centre, randomised trial. SETTING: National Maternity Hospital, Dublin, Ireland. POPULATION: A total of 120 women sustaining a primary third-degree tear. METHODS: Women were randomised in a one to three ratio: 30 to early postpartum home biofeedback physiotherapy and 90 to PFEs. MAIN OUTCOME MEASURES: Differences in anorectal manometry results, Cleveland Clinic continence scores and Rockwood faecal incontinence quality of life scale scores after 3 months of postpartum treatment. RESULTS: The mean anal resting pressure was 39 ± 13 mmHg in the early biofeedback physiotherapy group and 43 ± 17 mmHg in the PFE group. The mean anal squeeze pressure was 64 ± 17 mmHg in the biofeedback group and 62 ± 23 mmHg in the PFE group. There was no significant difference in anal resting and squeeze pressure values between the groups (P = 0.123 and P = 0.68, respectively). There were no differences in symptom score and quality of life measurements between the groups. CONCLUSIONS: This study demonstrates no added value in using early home biofeedback physiotherapy in the management of women sustaining third-degree tears. Poor compliance may have contributed because women found it difficult to designate time to using biofeedback.


Subject(s)
Anal Canal/injuries , Biofeedback, Psychology , Delivery, Obstetric/adverse effects , Exercise Therapy/methods , Lacerations/therapy , Perineum/injuries , Anal Canal/physiology , Episiotomy , Fecal Incontinence/prevention & control , Female , Humans , Lacerations/etiology , Manometry , Parturition , Pelvic Floor/physiology , Postpartum Period , Quality of Life , Surveys and Questionnaires
12.
Tech Coloproctol ; 15(4): 451-4, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21984050

ABSTRACT

BACKGROUND: Ileal pouch-anal anastomosis (IPAA) following proctocolectomy is the preferred option for patients with medically refractory ulcerative colitis, indeterminate colitis, and familial adenomatous polyposis. However, it remains a procedure associated with morbidity and mortality. Pelvic sepsis, pouch fistulae, and anastomotic dehiscence predispose to pouch failure. We report our experience with an adaptation for the formation of the stapled ileal J pouch using the GIA™ 100 stapling device (Covidien, Mansfield, Massachusetts, USA). When creating the J pouch, we remove the bevelled plastic protector from the thin fork of the stapling device, allowing the staple line to be completed to the tip of the stapled efferent limb of the pouch, thereby minimizing potential blind ending in the efferent limb and injury to the transverse staple line. METHODS: Patients undergoing elective IPAA at our institution over a 5-year period using this adapted stapling technique for creation of the ileal J pouch were reviewed. Data were collected from a prospectively maintained inflammatory bowel disease database, theater records, and patient chart review. RESULTS: Forty-one patients underwent IPAA using this technique at our institution during the study period. Postoperative morbidity was encountered in 11 of 41 patients including pelvic sepsis, pouch fistulae, anastomotic stricture, or leak. There was no morbidity observed related to a blind efferent limb or transverse staple line disruption. No mortality was observed in this series. CONCLUSION: Maximizing the length of the efferent fork of the GIA stapling device can reduce the length of redundant efferent J limb of the ileal J pouch. This may reduce the incidence of torsion, volvulus, distension, fistulae/sinuses, and pelvic sepsis/anastomotic leak following IPAA.


Subject(s)
Colonic Pouches , Inflammatory Bowel Diseases/surgery , Proctocolectomy, Restorative/methods , Quality of Life , Surgical Stapling/instrumentation , Anal Canal/surgery , Equipment Design , Female , Follow-Up Studies , Humans , Ireland/epidemiology , Male , Postoperative Complications/epidemiology , Prospective Studies , Time Factors , Treatment Outcome
13.
Br J Surg ; 98(8): 1155-9, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21618494

ABSTRACT

BACKGROUND: An experimental model of neuropathic faecal incontinence has recently been established. This study aimed to quantify and compare the effect of crush and compression injury on first-order sensory neurones of the inferior rectal nerve (IRN) using a nuclear marker of axonal injury, activating transcription factor (ATF) 3. METHODS: Eighteen Wistar rats were allocated to three groups: an unoperated control group, an IRN crush group (positive control) and a retrouterine balloon compression group. Five days after surgery, all animals were anaesthetized and perfused with fixative, and S1 dorsal root ganglia (DRG) were harvested. The tissue was sampled and neuronal nuclear ATF-3 expression calculated. RESULTS: Estimated total S1 DRG ATF-3 nuclear labelling was higher in the nerve crush (median (interquartile range) 171 (60-824) cells) and balloon compression (59 (20-274) cells) groups, compared with that in the unoperated control group (9 (3-24) cells) (P = 0.001 and P = 0.008 respectively). In all groups, most neurones displaying the marker of injury were of the C-fibre class. CONCLUSION: This study confirmed the presence of axonal injury in a pelvic compression model of obstetric injury. C-fibre afferent pathways appeared to be most vulnerable. Neuromodulation may function through augmentation of residual C-fibre pathways.


Subject(s)
Activating Transcription Factor 3/metabolism , Fecal Incontinence/etiology , Ganglia, Spinal/injuries , Animals , Biomarkers/metabolism , Catheterization , Female , Ganglia, Spinal/metabolism , Nerve Crush , Rats , Rats, Wistar
15.
Colorectal Dis ; 12(7 Online): e153-7, 2010 Jul.
Article in English | MEDLINE | ID: mdl-19674025

ABSTRACT

BACKGROUND: Pudendal nerve injury during childbirth may result in external anal sphincter (EAS) atrophy. Recently, balloon compression of the pelvic side wall has been shown to result in EAS atrophy in an experimental model. The aim of this study was to determine whether other sphincters of continence are similarly affected. METHOD: Sixteen adult female virgin Wistar rats (eight controls) were studied 4 weeks after surgery. Anal and urethral canals were dissected, snap frozen and sectioned using a cryostat (100 microm thickness). Masses of EAS, internal anal sphincter (IAS) and external urethral sphincter (EUS) were calculated stereologically and stained with succinate dehydrogenase histochemistry to differentiate striated from smooth muscle. Sphincter length was determined and total sphincter mass calculated. Data were analysed with an unpaired Student's t-test. RESULTS: Atrophy of EAS (30.9 +/- 1.7 to 21.3 +/- 1.7 mg/ kg, P = 0.001), IAS (98.1 +/- 11.3 to 67.1 +/- 5.0 mg/kg, P = 0.01) and EUS (9.6 +/- 0.98 to 7.4 +/- 0.8 mg/kg, P = 0.05) was found 4 weeks after the injury. CONCLUSION: In an experimental model of obstetric pudendal nerve injury, significant atrophy of striated and smooth muscle sphincters of continence occurs and may contribute to altered continence following vaginal childbirth.


Subject(s)
Anal Canal/pathology , Defecation/physiology , Fecal Incontinence/pathology , Anal Canal/innervation , Anal Canal/physiopathology , Animals , Atrophy , Disease Models, Animal , Fecal Incontinence/etiology , Fecal Incontinence/physiopathology , Female , Muscle, Smooth/innervation , Muscle, Smooth/pathology , Peripheral Nerve Injuries , Rats , Rats, Wistar , Severity of Illness Index
16.
J Urol ; 150(3): 1010-3, 1993 Sep.
Article in English | MEDLINE | ID: mdl-8345578

ABSTRACT

The effect of exogenous atrial natriuretic peptide (ANP) on urine excretion and glomerular filtration rate (GFR) during acute renal allograft rejection was evaluated in a canine model. Eight animals underwent simultaneous allotransplantation and unilateral native nephrectomy. No preoperative or postoperative immunosuppressive therapy was given. Acute renal function studies were performed on the allografts and companion, native kidneys following surgical exposure and mobilization on the third postoperative day. At reexploration, the allografts were found to be grossly enlarged (138 +/- 10 gm.) and contained moderate-to-marked perivascular interstitial infiltration. Glomerular filtration rate, determined by measurement of urinary inulin clearance, was significantly reduced from prenephrectomy baseline values (19 +/- 4 ml. per minute versus 32 +/- 5 ml. per minute, p < .05). During a 30 minute, intravenous ANP infusion, allograft urine flow rates increased from 1.4 +/- 0.5 ml. per minute to 3.3 +/- 0.4 ml. per minute (p < .01), and GFR increased from 19 +/- 4 ml. per minute to 24 +/- 4 ml. per minute (p < .05). During ANP infusion, mean arterial pressure declined from 136 +/- 7 mm. Hg to 116 +/- 7 mm. Hg (p < .05), and the hematocrit remained unchanged. These observations are consistent with previously described, ameliorative effects of ANP in other models of acute ischemic renal injury and provide an experimental basis for more extended studies examining the potential usefulness of ANP as adjunctive therapy in the treatment of acute renal allograft rejection.


Subject(s)
Atrial Natriuretic Factor/pharmacology , Glomerular Filtration Rate/drug effects , Graft Rejection/physiopathology , Kidney Transplantation/physiology , Urodynamics/drug effects , Acute Disease , Animals , Dogs , Graft Rejection/pathology
17.
Psychiatry Res ; 36(2): 157-67, 1991 Feb.
Article in English | MEDLINE | ID: mdl-2017530

ABSTRACT

Thirty depressed psychiatric inpatients, including 18 with a diagnosis of major depression, and 25 hospital staff controls were compared with respect to cellular immune function--that is, mitogen responsiveness to concanavalin A (con A), phytohemagglutinin (PHA), and pokeweed mitogen (PWM); natural killer cell (NK) activity; and T cell subsets, including helper/inducer T cells (CD4) and suppressor/cytotoxic cells (CD8). Only physically healthy subjects, who had not used psychoactive medications (except for low dose benzodiazepines) or other medications known to affect the immune system for at least 14 days, were included. Paired comparisons of the immune measures of patients with a DSM-III diagnosis of major depression (n = 18) with their controls demonstrated a statistically significant reduction of the patients' con A response. In addition, the patients with major depression had significantly lower con A and PHA responses than the combined patients with other forms of depression (atypical, dysthymic, or atypical bipolar). There was no indication that severity of depression, dexamethasone suppression test status, benzodiazepine use, or age accounted for the differences in immune function. A possibly important, unexpected finding was that antihistamine use was associated with lower immune function.


Subject(s)
Depressive Disorder/immunology , Hospitalization , Lymphocyte Activation , Adult , Aged , Depressive Disorder/diagnosis , Depressive Disorder/psychology , Dexamethasone , Female , Humans , Hydrocortisone/blood , Killer Cells, Natural/immunology , Male , Middle Aged , Mitogens , Psychiatric Status Rating Scales , Severity of Illness Index , T-Lymphocytes/immunology
18.
J Bacteriol ; 168(2): 785-90, 1986 Nov.
Article in English | MEDLINE | ID: mdl-3782025

ABSTRACT

We constructed a genetic map of the fla-che region of the Rhizobium meliloti chromosome using cotransduction with bacteriophage phi M12. Several other chromosomal markers located in the general area are included in the map. We isolated plasmids carrying wild-type DNA inserts that complement the mapped mutations from a genomic library carried in the broad-host-range vector pLAFR1. The complementation data obtained from the clones confirmed the contransduction map and clarified the exact order of several of the behavioral genes. A restriction map of this area was developed by using the cloned DNA. One of the five individual EcoRI fragments subcloned from the original clones complemented two of the behavioral mutations.


Subject(s)
Genes, Bacterial , Rhizobium/genetics , Cell Movement , Chemotaxis , Chromosome Mapping , Chromosomes, Bacterial , Cloning, Molecular , Flagella , Genetic Complementation Test , Mutation , Rhizobium/physiology , Rhizobium/ultrastructure , Transduction, Genetic
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