Clubfoot: Congenital Talipes Equinovarus.

Congenital talipes equinovarus (CTEV), also known as clubfoot, is a common musculoskeletal entity that affects one to two per 1000 live births worldwide. Imaging modalities including radiography, US, and MRI have emerged as valuable tools for the diagnosis, treatment, and monitoring of CTEV. The deformity is characterized by midfoot cavus, forefoot adductus, and hindfoot varus and equinus. The Ponseti method of manipulation and serial casting is the standard treatment of CTEV. Radiography shows the anatomy, position, and relationships of the different bones of the foot. US allows accurate assessment of cartilaginous and bony structures, in addition to its inherent advantages such as absence of ionizing radiation exposure. One of the indications for US is to monitor the response to Ponseti method treatment. MRI enables visualization of bones, cartilage, and soft tissues and allows multiplanar evaluation of deformities, providing a comprehensive imaging analysis of CTEV. An integrated approach that combines clinical examination and imaging findings is essential for effective management of CTEV. The authors provide a comprehensive overview of CTEV with a review of imaging modalities to help evaluate CTEV, focusing on radiography, US, and MRI. Using this article as a guide, radiologists involved in the assessment and treatment of CTEV can contribute to the management of the condition. ©RSNA, 2024 Supplemental material is available for this article.

Fungal Musculoskeletal Infections: Comprehensive Approach to Proper Diagnosis.

Fungal musculoskeletal infections often have subacute or indolent manifestations, making it difficult to distinguish them from other diseases and infections, given that they are relatively uncommon. Fungal infections occur by hematogenous spread, direct inoculation, or contiguous extension and may be related to different risk factors, including immunosuppression and occupational activity. The infection can manifest in isolation in the musculoskeletal system or as part of a systemic process. The fungi may be endemic to certain regions or may be found throughout the world, and this can help to narrow the diagnosis of the etiologic agent. Infections such as candidiasis, cryptococcosis, aspergillosis, and mucormycosis are often related to immunosuppression. On the other hand, histoplasmosis, paracoccidioidomycosis, coccidioidomycosis, and blastomycosis can occur in healthy patients in geographic areas where these infections are endemic. Furthermore, infections can be classified on the basis of the site of infection in the body. Some subcutaneous infections that can have osteoarticular involvement include mycetoma, sporotrichosis, and phaeohyphomycosis. Different fungi affect specific bones and joints with greater prevalence. Imaging has a critical role in the evaluation of these diseases. Imaging findings include nonspecific features such as osteomyelitis and arthritis, with bone destruction, osseous erosion, mixed lytic and sclerotic lesions, and joint space narrowing. Multifocal osteomyelitis and chronic arthritis with joint effusion and synovial thickening may also occur. Although imaging findings are often nonspecific, some fungal infections may show findings that aid in narrowing the differential diagnosis, especially when they are associated with the patient's clinical condition and history, the site of osteoarticular involvement, and the geographic location. ©RSNA, 2024.

Acute administration of a dopamine D2/D3 receptor agonist alters behavioral and neural parameters in adult zebrafish.

The dopaminergic neurotransmitter system is implicated in several brain functions and behavioral processes. Alterations in it are associated with the pathogenesis of several human neurological disorders. Pharmacological agents that interact with the dopaminergic system allow the investigation of dopamine-mediated cellular and molecular responses and may elucidate the biological bases of such disorders. Zebrafish, a translationally relevant biomedical research organism, has been successfully employed in prior psychopharmacology studies. Here, we evaluated the effects of quinpirole (dopamine D2/D3 receptor agonist) in adult zebrafish on behavioral parameters, brain-derived neurotrophic factor (BDNF) and neurotransmitter levels. Zebrafish received intraperitoneal injections of 0.5, 1.0, or 2.0 mg/kg quinpirole or saline (control group) twice with an inter-injection interval of 48 h. All tests were performed 24 h after the second injection. After this acute quinpirole administration, zebrafish exhibited decreased locomotor activity, increased anxiety-like behaviors and memory impairment. However, quinpirole did not affect social and aggressive behavior. Quinpirole-treated fish exhibited stereotypic swimming, characterized by repetitive behavior followed by immobile episodes. Moreover, quinpirole treatment also decreased the number of BDNF-immunoreactive cells in the zebrafish brain. Analysis of neurotransmitter levels demonstrated a significant increase in glutamate and a decrease in serotonin, while no alterations were observed in dopamine. These findings demonstrate that dopaminergic signaling altered by quinpirole administration results in significant behavioral and neuroplastic changes in the central nervous system of zebrafish. Thus, we conclude that the use of quinpirole administration in adult zebrafish may be an appropriate tool for the analysis of mechanisms underlying neurological disorders related to the dopaminergic system.

Work as Meaning Inventory: Psychometric Properties and Additional Evidence of the Brazilian Version / Work as Meaning Inventory: Propriedades Psicométricas e Evidências Adicionais da Versão Brasileira / Inventario del Trabajo como Significado: Propiedades Psicométricas y Evidencias Adicionales de la Versión Brasileña

Abstract Meaningful work promotes the professional development of individuals and organizations. This study aimed to translate, adapt, and present additional evidence of the validity of the Work as Meaning Inventory - WAMI for the Brazilian population. It addressed 2,111 workers (67% women) aged between 18 and 77. The results indicate that the inventory's Brazilian version has a linguistic structure appropriate to the Brazilian Portuguese and satisfactory goodness of fit indices for the one-factor structure, in addition to invariance across sexes. Additionally, evidence of external convergent validity was found with life satisfaction and job satisfaction, which was negative for exhaustion. The conclusion is that meaningful work benefits the well being of workers and organizations. Thus, we recommend that this adapted version be used in the Brazilian context, as it meets the psychometric requirements for adapting instruments across cultures.

Resumo Trabalho significativo promove o desenvolvimento profissional dos indivíduos e organizações. Este estudo teve por objetivo traduzir, adaptar e apresentar evidências adicionais de validade do Work as Meaning Inventory - WAMI para a população brasileira. Participaram do estudo 2.111 trabalhadores (67% mulheres) com idades entre 18 a 77 anos. Os resultados indicaram que a versão brasileira do inventário apresentou estrutura linguística ao idioma português do Brasil adequada, melhores índices de ajuste na estrutura unifatorial e invariância por sexo. Evidências externas de validade convergentes com satisfação com a vida, satisfação laboral e negativa com exaustão incrementam informações do WAMI. Conclui-se que o trabalho significativo traz benefícios ao bem-estar dos trabalhadores e organizações. Por fim, recomenda-se o uso desta versão adaptada para o contexto brasileiro, por atender aos rigores psicométricos de adaptação de instrumentos entre culturas.

Resumen El trabajo significativo promueve el desarrollo profesional de las personas y las organizaciones. Este estudio tuvo como objetivo traducir, adaptar y presentar evidencias adicionales de validez del Work as Meaning Inventory - WAMI para la población brasileña. El estudio incluyó a 2111 trabajadores (67% mujeres) con edades entre 18 y 77 años. Los resultados de validez externa convergente con la satisfacción con la indicaron que la versión brasileña del inventario presentó estructura lingüística adecuada al portugués brasileño, mejores índices de ajuste en la estructura unifactorial e invariancia por sexo. La evidencia vida, la satisfacción laboral y negativa con el agotamiento aumentan la información del WAMI. Se concluye que el trabajo significativo trae beneficios para el bienestar de los trabajadores y las organizaciones. Finalmente, se recomienda el uso de esta versión adaptada para el contexto brasileño, ya que cumple con los rigores psicométricos de la adaptación de instrumentos entre culturas.

Long-lasting behavioral effects of quinpirole exposure on zebrafish.

The human brain matures into a complex structure, and to reach its complete development, connections must occur along exact paths. If at any stage, the processes are altered, interrupted, or inhibited, the consequences can be permanent. Dopaminergic signaling participates in the control of physiological functions and behavioral processes, and alterations in this signaling pathway are related to the pathogenesis of several neurological disorders. For this reason, the use of pharmacological agents able to interact with the dopaminergic signaling may elucidate the biological bases of such disorders. We investigated the long-lasting behavioral effects on adult zebrafish after quinpirole (a dopamine D2/D3 receptor agonist) exposure during early life stages of development (24 h exposure at 5 days post-fertilization, dpf) to better understand the mechanisms underlying neurological disorders related to the dopaminergic system. Quinpirole exposure at the early life stages of zebrafish led to late behavioral alterations. When evaluated at 120 dpf, zebrafish presented increased anxiety-like behaviors. At the open tank test, fish remained longer at the bottom of the tank, indicating anxiety-like behavior. Furthermore, quinpirole-treated fish exhibited increased absolute turn angle, likely an indication of elevated erratic movements and a sign of increased fear or anxiety. Quinpirole-treated fish also showed altered swimming patterns, characterized by stereotypic swimming. During the open tank test, exposed zebrafish swims from corner to corner in a repetitive manner at the bottom of the tank. Moreover, quinpirole exposure led to memory impairment compared to control fish. However, quinpirole administration had no effects on social and aggressive behavior. These findings demonstrate that dopaminergic signaling altered by quinpirole administration in the early life stages of development led to late alterations in behavioral parameters of adult zebrafish.

Structural and Evolutionary Analyses of PR-4 SUGARWINs Points to a Different Pattern of Protein Function.

SUGARWINs are PR-4 proteins associated with sugarcane defense against phytopathogens. Their expression is induced in response to damage by Diatraea saccharalis larvae. These proteins play an important role in plant defense, in particular against fungal pathogens, such as Colletothricum falcatum (Went) and Fusarium verticillioides. The pathogenesis-related protein-4 (PR-4) family is a group of proteins equipped with a BARWIN domain, which may be associated with a chitin-binding domain also known as the hevein-like domain. Several PR-4 proteins exhibit both chitinase and RNase activity, with the latter being associated with the presence of two histidine residues H11 and H113 (BARWIN) [H44 and H146, SUGARWINs] in the BARWIN-like domain. In sugarcane, similar to other PR-4 proteins, SUGARWIN1 exhibits ribonuclease, chitosanase and chitinase activities, whereas SUGARWIN2 only exhibits chitosanase activity. In order to decipher the structural determinants involved in this diverse range of enzyme specificities, we determined the 3-D structure of SUGARWIN2, at 1.55Å by X-ray diffraction. This is the first structure of a PR-4 protein where the first histidine has been replaced by asparagine and was subsequently used to build a homology model for SUGARWIN1. Molecular dynamics simulations of both proteins revealed the presence of a flexible loop only in SUGARWIN1 and we postulate that this, together with the presence of the catalytic histidine at position 42, renders it competent as a ribonuclease. The more electropositive surface potential of SUGARWIN1 would also be expected to favor complex formation with RNA. A phylogenetic analysis of PR-4 proteins obtained from 106 Embryophyta genomes showed that both catalytic histidines are widespread among them with few replacements in these amino acid positions during the gene family evolutionary history. We observe that the H11 replacement by N11 is also present in two other sugarcane PR-4 proteins: SUGARWIN3 and SUGARWIN4. We propose that RNase activity was present in the first Embryophyta PR-4 proteins but was recently lost in members of this family during the course of evolution.

Large-Scale Deployment and Establishment of Wolbachia Into the Aedes aegypti Population in Rio de Janeiro, Brazil.

Traditional methods of vector control have proven insufficient to reduce the alarming incidence of dengue, Zika, and chikungunya in endemic countries. The bacterium symbiont Wolbachia has emerged as an efficient pathogen-blocking and self-dispersing agent that reduces the vectorial potential of Aedes aegypti populations and potentially impairs arboviral disease transmission. In this work, we report the results of a large-scale Wolbachia intervention in Ilha do Governador, Rio de Janeiro, Brazil. wMel-infected adults were released across residential areas between August 2017 and March 2020. Over 131 weeks, including release and post-release phases, we monitored the wMel prevalence in field specimens and analyzed introgression profiles of two assigned intervention areas, RJ1 and RJ2. Our results revealed that wMel successfully invaded both areas, reaching overall infection rates of 50-70% in RJ1 and 30-60% in RJ2 by the end of the monitoring period. At the neighborhood-level, wMel introgression was heterogeneous in both RJ1 and RJ2, with some profiles sustaining a consistent increase in infection rates and others failing to elicit the same. Correlation analysis revealed a weak overall association between RJ1 and RJ2 (r = 0.2849, p = 0.0236), and an association at a higher degree when comparing different deployment strategies, vehicle or backpack-assisted, within RJ1 (r = 0.4676, p < 0.0001) or RJ2 (r = 0.6263, p < 0.0001). The frequency knockdown resistance (kdr) alleles in wMel-infected specimens from both areas were consistently high over this study. Altogether, these findings corroborate that wMel can be successfully deployed at large-scale as part of vector control intervention strategies and provide the basis for imminent disease impact studies in Southeastern Brazil.

Effectiveness of Wolbachia-infected mosquito deployments in reducing the incidence of dengue and other Aedes-borne diseases in Niterói, Brazil: A quasi-experimental study.

BACKGROUND: The introduction of the bacterium Wolbachia (wMel strain) into Aedes aegypti mosquitoes reduces their capacity to transmit dengue and other arboviruses. Evidence of a reduction in dengue case incidence following field releases of wMel-infected Ae. aegypti has been reported previously from a cluster randomised controlled trial in Indonesia, and quasi-experimental studies in Indonesia and northern Australia. METHODOLOGY/PRINCIPAL FINDINGS: Following pilot releases in 2015-2016 and a period of intensive community engagement, deployments of adult wMel-infected Ae. aegypti mosquitoes were conducted in Niterói, Brazil during 2017-2019. Deployments were phased across four release zones, with a total area of 83 km2 and a residential population of approximately 373,000. A quasi-experimental design was used to evaluate the effectiveness of wMel deployments in reducing dengue, chikungunya and Zika incidence. An untreated control zone was pre-defined, which was comparable to the intervention area in historical dengue trends. The wMel intervention effect was estimated by controlled interrupted time series analysis of monthly dengue, chikungunya and Zika case notifications to the public health surveillance system before, during and after releases, from release zones and the control zone. Three years after commencement of releases, wMel introgression into local Ae. aegypti populations was heterogeneous throughout Niterói, reaching a high prevalence (>80%) in the earliest release zone, and more moderate levels (prevalence 40-70%) elsewhere. Despite this spatial heterogeneity in entomological outcomes, the wMel intervention was associated with a 69% reduction in dengue incidence (95% confidence interval 54%, 79%), a 56% reduction in chikungunya incidence (95%CI 16%, 77%) and a 37% reduction in Zika incidence (95%CI 1%, 60%), in the aggregate release area compared with the pre-defined control area. This significant intervention effect on dengue was replicated across all four release zones, and in three of four zones for chikungunya, though not in individual release zones for Zika. CONCLUSIONS/SIGNIFICANCE: We demonstrate that wMel Wolbachia can be successfully introgressed into Ae. aegypti populations in a large and complex urban setting, and that a significant public health benefit from reduced incidence of Aedes-borne disease accrues even where the prevalence of wMel in local mosquito populations is moderate and spatially heterogeneous. These findings are consistent with the results of randomised and non-randomised field trials in Indonesia and northern Australia, and are supportive of the Wolbachia biocontrol method as a multivalent intervention against dengue, chikungunya and Zika.

Efeitos da meditação sobre os sintomas da ansiedade: uma revisão sistemática / Effects of meditation on anxiety symptoms: a systematic review

INTRODUÇÃO: A Ansiedade é um estado subjetivo de medo, apreensão ou tensão em relação a algo desconhecido ou estranho que pode acarretar desconforto psicológico e/ou físico caso ocorra de forma exacerbada e que, segundo a Organização Mundial da Saúde, afeta significativamente a população brasileira. É importante lidar com este transtorno com o objetivo de diminuir os danos físicos e psicológicos ele causa nos indivíduos; para isso, as estratégias de tratamento mais utilizadas são a intervenção medicamentosa e tratamentos não farmacológicos como a terapia, atividade física e a meditação. OBJETIVO: Estudar os efeitos da meditação no tratamento da ansiedade. METODOLOGIA: Trata-se de uma revisão sistemática de estudos intervencionais. As bases MEDLINE (Pubmed) e SCIELO foram utilizadas para a coleta de artigos em inglês e português, publicados entre os anos de 2010 e 2020. RESULTADOS: Oito ensaios clínicos randomizados foram selecionados. Os estudos sugerem uma associação positiva entre a prática da meditação e a melhora dos sintomas físicos e psicológicos dos pacientes portadores de transtorno de ansiedade. CONCLUSÃO: De modo geral, a literatura científica evidencia os efeitos benéficos da meditação no tratamento da ansiedade, embora se façam necessárias mais pesquisas sobre o tema.

INTRODUCTION: Anxiety is a subjective state of fear, apprehension, or tension in relation to something unknown or strange that can cause psychological and / or physical discomfort if it occurs in an exacerbated way and, according to the World Health Organization, that represents significant bad effects to the Brazilian population. It is important to deal with this disorder to reduce the physical and psychological damage it causes to individuals; for this, the most used treatment strategies are drug intervention and non-pharmacological treatments such as therapy, physical activity, and meditation. OBJECTIVE: To study the effects of meditation in the treatment of anxiety. METHODOLOGY: This is a systematic review of interventional studies. The MEDLINE (Pubmed) and SCIELO databases were used to collect articles in English and Portuguese, published between 2010 and 2020. RESULTS: Eight randomized clinical trials were selected. Studies suggest a positive association between meditation practice and improved physical and psychological symptoms of patients with anxiety disorder. CONCLUSION: In general, the scientific literature highlights the beneficial effects of meditation in treating anxiety, although more research on the topic is needed.

SARS-CoV-2 mutations in Brazil: from genomics to putative clinical conditions.

Due to the high rate of transmissibility, Brazil became the new COVID-19 outbreak epicenter and, since then, is being monitored to understand how SARS-CoV-2 mutates and spreads. We combined genomic and structural analysis to evaluate genomes isolated from different regions of Brazil and show that the most prevalent mutations were located in the S, N, ORF3a and ORF6 genes, which are involved in different stages of viral life cycle and its interaction with the host cells. Structural analysis brought to light the positions of these mutations on protein structures, contributing towards studies of selective structure-based drug discovery and vaccine development.

Reduced competence to arboviruses following the sustainable invasion of Wolbachia into native Aedes aegypti from Southeastern Brazil.

Field release of Wolbachia-infected Aedes aegypti has emerged as a promising solution to manage the transmission of dengue, Zika and chikungunya in endemic areas across the globe. Through an efficient self-dispersing mechanism, and the ability to induce virus-blocking properties, Wolbachia offers an unmatched potential to gradually modify wild Ae. aegypti populations turning them unsuitable disease vectors. Here we describe a proof-of-concept field trial carried out in a small community of Niterói, greater Rio de Janeiro, Brazil. Following the release of Wolbachia-infected eggs, we report here a successful invasion and long-term establishment of the bacterium across the territory, as denoted by stable high-infection indexes (> 80%). We have also demonstrated that refractoriness to dengue and Zika viruses, either thorough oral-feeding or intra-thoracic saliva challenging assays, was maintained over the adaptation to the natural environment of Southeastern Brazil. These findings further support Wolbachia's ability to invade local Ae. aegypti populations and impair disease transmission, and will pave the way for future epidemiological and economic impact assessments.

Feeding status alters exploratory and anxiety-like behaviors in zebrafish larvae exposed to quinpirole.

The dysfunction of dopaminergic signaling is associated with several neurological disorders. The use of pharmacological agents that interact with this signaling system may be employed to understand mechanisms underlying such disorders. Nutritional status can impact dopamine reuptake, receptor affinity, transporter activity, and the effects of drugs that bind to dopamine receptors or interact with dopaminergic system. Here we evaluated the effects of quinpirole (a dopamine D2/D3 receptor agonist) exposure on fed and non-fed zebrafish larvae. Zebrafish larvae (6 days post-fertilization, dpf) were exposed to quinpirole (5.5, 16.7, and 50.0 µM) or water (control group) for one hour. To evaluate the effect of feeding status on quinpirole exposure, the experiments were performed on fed and non-fed animals, a between subject experimental design. Both fed and non-fed quinpirole treated larvae exhibited increased erratic movements compared to controls in an open tank exploration task. No alterations were observed on the main parameters of exploratory behavior and swim activity for non-fed larvae treated with quinpirole compared to controls. However, fed animals exposed to quinpirole exhibited increased locomotor activity, anxiety-like behavior, and repetitive circular movements when compared to controls and non-fed exposed animals. In addition, we observed quinpirole exposure to have no effects on morphological parameters and heartbeat, but to impair optomotor responses in both fed and non-fed larvae compared to control. We also found quinpirole effects to interact with feeding status, as quinpirole-treated fed larvae improved while quinpirole treated non-fed larvae impaired their avoidance reaction towards an aversive stimulus. These results indicate that the behavioral effects of quinpirole exposure depended upon feeding status. They showed that consumption of food, a naturally rewarding stimulus known to engage the dopaminergic system, made this neurotransmitter system more susceptible to quinpirole's effects.

Reproducibility on science: Challenges and advances in Brazilian alcohol surveys.

BACKGROUND: Reproducibility in Science is challenging and may be hard to achieve in alcohol research. Previous general population surveys in Brazil have estimated the prevalence of alcohol dependence to be around 10%. We aim to estimate alcohol use and dependence using different methods and definitions. METHODS: The 3rd Brazilian Household Survey on Substance Use (BHSU-3) was a nationwide, probability sample survey that interviewed 16,273 individuals. DSM-IV-TR criteria were used to determine alcohol dependence. In the BHSU-2 (covering only Brazil's 108 largest municipalities), alcohol dependence was defined as fulfilling 2/6 DSM-III criteria. Using the BHSU-3 data, alcohol use was estimated at: [1] the national level, [2] BHSU-2 municipalities, taking into consideration the sample design, and [3] BHSU-2 municipalities, ignoring the sample design. Alcohol dependence was calculated using: BHSU-3 and BHSU-2 definitions, two denominators ([A] population and [B] 12-month drinkers), and [1], [2], [3]. RESULTS: Lifetime alcohol use ranged from 66.4% (95%CI:64.8-68.0 [1]) to 70.1% ([95%CI:69.1-71.0], [3]). The estimated population presenting with alcohol dependence ranged from N = 2.3 million (BHSU-3 definition, [1]) to N = 4.3 million (BHSU-2 definition, [1]). In the first case, the prevalence among the general population [A] and drinkers [B] was 1.5% (95%CI:1.2-1.8) and 3.5% (95%CI:2.8-4.2), respectively. In second case, prevalence was 2.8% (95%CI:2.4-3.3, [A]) and 6.6% (95%CI:5.6-7.6, [B]). CONCLUSIONS: Prevalence of alcohol dependence may vary as much as 4.3 times, analyzing the same dataset in different ways. Brazilian research on alcohol is funded by governmental research and policy-making agencies, providing subsidies for alcohol policy in the country. It is crucial that sufficient methodological information is provided in order to guarantee reproducibility and consistency over time.

Feeding Behavior of Mangalarga Marchador Weanlings Fed Sorghum Silage Versus Grass Hay.

Mangalarga Marchador foals in Minas Gerais, Brazil, are usually weaned during the dry season, when there is a scarcity of forage. Although sorghum silage has been used to feed weanlings, the effect on their feeding behavior in comparison with grass hay has not been reported. Twelve Mangalarga Marchador weanlings were randomly assigned to two groups of six animals: SS: sorghum silage; VH: Vaquero hay. Both groups received commercial concentrate in the proportion of 1.2 kg.100 kg-1 of the live weight and forage ad libitum. The animals were filmed for 48 hours, being timed: forage intake time, water intake time (WIT), concentrate intake time, and leisure time (LT). Tukey's test was used to compare the parameters between SS and VH groups. Forage intake time and WIT were higher (P < .05) in VH group because the hay had low moisture content, stimulating water intake. Feeding behavior of weanlings kept in individual stalls and consuming sorghum silage was different than those consuming grass hay, providing less forage intake time and longer LT spent in comparison with VH.

Factorial structure of the Frugality Scale: exploratory evidence / Estrutura fatorial da Escala de Frugalidade: evidências exploratórias / La estructura factorial de la Escala de Frugalidad: evidencia exploratorios

This article aims to promote an investigation of the psychometric properties of Frugality Scale adapted to Brazilian Portuguese. Frugality is characterized by using and consuming goods in a resourceful way, fully exploring its durability as well. To this end, 626 people responded to an online form that contained the frugality scale. Procedures such as non-graphical solutions to the scree plot, exploratory graphical analysis and a Schmid-Leiman factor solution point to evidence that the scale's structure is not one-dimensional but two-dimensional. Finally, modeling strategies also indicate that a bifactor solution can be applied. (AU)

Este artigo tem por objetivo promover uma investigação das propriedades psicométricas da Escala de Frugalidade (Lastovicka et al., 1999) adaptada para o português brasileiro. Frugalidade é caracterizada pelo controle em consumir e pelo uso pleno de recursos. Com esse objetivo, 626 pessoas responderam a um formulário on-line que continha a Escala de Frugalidade. Foram utilizados quatro procedimentos com o objetivo de investigar a estrutura da escala de frugalidade por um framework exploratório. Procedimentos como as soluções não gráficas para o scree plot, análise gráfica exploratória e a solução fatorial Schmid-Leiman apontam para a possibilidade da escala não ser unidimensional, mas bidimensional. As estratégias de modelagem da estrutura da escala apontam que um modelo bifator pode ser aplicado. (AU)

Este artículo tiene como objetivo promover una investigación de las propiedades psicométricas de la Escala de Frugalidad (Lastovicka et al.,1999) adaptada al portugués de Brasil. La frugalidad se caracteriza por el control en consumir y la plena utilización de los recursos. Con este objetivo, 626 personas respondieron a un formulario online que contenía la Escala de Frugalidad. Se utilizaron cuatro procedimientos con el objetivo de investigar la estructura de la Escala de Frugalidad por un framework exploratorio. Los procedimientos como las soluciones no gráficas para el scree plot, el análisis gráfico exploratorio y la solución factorial Schmid-Leiman apuntan hacia la posibilidad de que la escala no sea unidimensional, sino bidimensional. Las estrategias de modelado de la estructura de la escala señalan que un modelo bifactor puede ser aplicado. (AU)

[Analysis of the geographical distribution of cases of leprosy. Rio de Janeiro, 2001-2012]. / Análise da distribuição geográfica dos casos de hanseníase. Rio de Janeiro, 2001 a 2012.

Studies have demonstrated that the geographical distribution of leprosy is related to different socioeconomic factors. This article aims to study the geographical distribution of leprosy in the state of Rio de Janeiro. The cases of leprosy reported in the 2001-2012 period were mapped according to municipality. Epidemiological and socioeconomic indicators were calculated. The ArcMap program was used for the construction of maps and Earth View to calculate the Bayesian rate. It was observed that leprosy is presented in hyper-endemic levels especially in the metropolitan area. However, there is also a reduction of the detection rate in the most recent study period. In municipalities in the metropolitan region and the north western region detection in children under 15 is high, indicating an active transmission situation. In municipalities in the south-central region and especially in the coastal region, there was a high proportion of cases diagnosed with level II disability, reflecting late diagnosis. There was no linear correlation between socioeconomic indicators and leprosy rate. These results contribute to the analysis of the geographical distribution of leprosy, important for the identification of areas for resource allocation, aiming to control and eliminate the disease.

Análise da distribuição geográfica dos casos de hanseníase. Rio de Janeiro, 2001 a 2012 / Analysis of the geographical distribution of cases of leprosy. Rio de Janeiro, 2001-2012

Resumo Trabalhos demonstraram que a distribuição geográfica da hanseníase está relacionada a diferentes fatores socioeconômicos. O objetivo deste artigo é estudar a distribuição geográfica da hanseníase no estado do Rio de Janeiro. Os casos de hanseníase notificados no período 2001-2012 foram mapeados segundo município. Foram calculados indicadores epidemiológicos e socioeconômicos. Utilizou-se o programa ArcMap para a construção dos mapas e o Terra View para o cálculo de taxa bayesiana. Observou-se que a hanseníase apresenta-se em níveis hiperendêmicos, especialmente na região metropolitana. No entanto, observa-se também uma redução do coeficiente de detecção no período mais recente do estudo. Em municípios da região metropolitana e da região noroeste a detecção em menores de 15 anos é elevada, indicando situação de transmissão ativa. Em municípios da região centro-sul e especialmente na baixada litorânea, observou-se elevada proporção de casos diagnosticados com grau II de incapacidade, refletindo alto índice de diagnóstico tardio. Não foi observada correlação linear entre os indicadores socioeconômicos e a detecção da hanseníase. Esses resultados contribuem para a análise da distribuição geográfica da hanseníase, importante para a identificação de áreas para alocação de recursos, visando controle e eliminação da doença.

Abstract Studies have demonstrated that the geographical distribution of leprosy is related to different socioeconomic factors. This article aims to study the geographical distribution of leprosy in the state of Rio de Janeiro. The cases of leprosy reported in the 2001-2012 period were mapped according to municipality. Epidemiological and socioeconomic indicators were calculated. The ArcMap program was used for the construction of maps and Earth View to calculate the Bayesian rate. It was observed that leprosy is presented in hyper-endemic levels especially in the metropolitan area. However, there is also a reduction of the detection rate in the most recent study period. In municipalities in the metropolitan region and the north western region detection in children under 15 is high, indicating an active transmission situation. In municipalities in the south-central region and especially in the coastal region, there was a high proportion of cases diagnosed with level II disability, reflecting late diagnosis. There was no linear correlation between socioeconomic indicators and leprosy rate. These results contribute to the analysis of the geographical distribution of leprosy, important for the identification of areas for resource allocation, aiming to control and eliminate the disease.

Mapping of the environmental contamination of Toxoplasma gondii by georeferencing isolates from chickens in an endemic area in Southeast Rio de Janeiro State, Brazil.

The environmental contamination of Toxoplasma gondii in an endemic area in Brazil was mapped by georeferencing isolates from chickens in farms in the Southeast of the state of Rio de Janeiro. Tissue samples obtained from 153 adult chickens were analyzed by the mouse bioassay for T. gondii infection. These animals were reared free-range on 51 farms in the municipalities of Rio Bonito and Maricá. The ArcGIS kernel density estimator based on the frequency of T. gondii-positive chickens was used to map the environmental contamination with this parasite. A questionnaire was applied to obtain data on the presence and management of cats and the type of water consumed. Of the farms studied, 64.7% were found to be located in areas of low to medium presence of T. gondii, 27.5% in areas with a high or very high contamination level and 7.8% in non-contaminated areas. Additionally, 70.6% kept cats, 66.7% were near water sources and 45.0% were in or near dense vegetation. Humans used untreated water for drinking on 41.2% of the farms, while all animals were given untreated water. The intensity of environmental T. gondii contamination was significantly higher on farms situated at a distance >500 m from water sources (P=0.007) and near (≤500 m) dense vegetation (P=0.003). Taken together, the results indicate a high probability of T. gondii infection of humans and animals living on the farms studied. The kernel density estimator obtained based on the frequency of chickens testing positive for T. gondii in the mouse bioassay was useful to map environmental contamination with this parasite.