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1.
Auton Autacoid Pharmacol ; 34(3-4): 27-34, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25702702

ABSTRACT

Preclinical and clinical studies show that gastrointestinal (GI) inflammation can evoke sensory changes occasionally far from the original inflammatory site. Animal models of colitis with either trinitrobenzenesulphonic acid (TNBS) or mustard oil (MO) produce distinct patterns of somatic and visceral sensory changes. We evaluated the effects of four doses of i.v. vincristine 150 µg kg(-1) (total of 600 µg kg(-1) ) treatment on the somatic (thermal nociceptive threshold) and colonic (morphological) changes induced by TNBS or MO in rats. TNBS and MO groups were further submitted to vincristine or saline pretreatments. TNBS induced somatic hypersensitivity, while MO induced somatic hyposensitivity (P < 0.05) when compared to the saline and ethanol control groups. Vincristine per se induced somatic hypersensitivity (P < 0.05). This effect was enhanced by TNBS and reversed by MO treatments. Although vincristine increased the colitis area (colonic weight length(-1) ratio) and the Morris' score in TNBS-treated rats, it did not alter the colitis area and even lowered the Morris' score in MO-treated rats. Compared to the saline (control) group, vincristine did not alter the colonic microscopic pattern. However, such lesions scores are higher (P < 0.05) in colitis groups induced by TNBS and MO, pretreated or not with vincristine. In conclusion, the somatic changes induced by different models of experimental colitis are diverse and modulated differently by vincristine.


Subject(s)
Colitis/drug therapy , Colitis/pathology , Colon/drug effects , Colon/pathology , Pain Threshold/drug effects , Vincristine/pharmacology , Vincristine/therapeutic use , Animals , Antineoplastic Agents, Phytogenic/pharmacology , Antineoplastic Agents, Phytogenic/therapeutic use , Disease Models, Animal , Drug Interactions , Male , Mustard Plant , Plant Oils , Rats , Severity of Illness Index , Trinitrobenzenesulfonic Acid
2.
Braz. j. med. biol. res ; 42(6): 567-573, June 2009. graf, tab
Article in English | LILACS | ID: lil-512767

ABSTRACT

We evaluated the effects of vincristine on the gastrointestinal (GI) motility of awake rats and correlated them with the course of vincristine-induced peripheral neuropathy. Vincristine or saline was injected into the tail vein of male Wistar rats (180-250 g) on alternate days: 50 µg/kg (5 doses, N = 10), 100 µg/kg (2, 3, 4 and 5 doses, N = 49) or 150 µg/kg (1, 2, or 5 doses, N = 37). Weight and stool output were measured daily for each animal. One day after completing the vincristine treatment, the animals were fasted for 24 h, gavage-fed with a test meal and sacrificed 10 min later to measure gastric emptying (GE), GI transit and colon weight. Sensory peripheral neuropathy was evaluated by hot plate testing. Chronic vincristine treatments with total cumulative doses of at least 250 µg/kg significantly decreased GE by 31-59 percent and GI transit by 55-93 percent. The effect of 5 doses of vincristine (150 µg/kg) on GE did not persist for more than 1 week. Colon weight increased after 2 and 5 doses of vincristine (150 µg/kg). Fecal output decreased up to 48 h after the fifth dose of vincristine (150 µg/kg). Vincristine decreased the heat pain threshold 1 day after 5 doses of 50-100 µg/kg or after 3-5 doses of 150 µg/kg. This effect lasted for at least 2 weeks after the fifth dose. Chronic intravenous vincristine treatment delayed GE and GI transit of liquid. This effect correlated with the peak increase in colon weight but not with the pain threshold changes.


Subject(s)
Animals , Male , Rats , Antineoplastic Agents, Phytogenic/pharmacology , Autonomic Nervous System Diseases/chemically induced , Gastric Emptying/drug effects , Gastrointestinal Transit/drug effects , Vincristine/pharmacology , Antineoplastic Agents, Phytogenic/administration & dosage , Dose-Response Relationship, Drug , Organ Size/drug effects , Pain Measurement/drug effects , Rats, Wistar , Time Factors , Vincristine/administration & dosage
3.
Braz J Med Biol Res ; 42(6): 567-73, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19448908

ABSTRACT

We evaluated the effects of vincristine on the gastrointestinal (GI) motility of awake rats and correlated them with the course of vincristine-induced peripheral neuropathy. Vincristine or saline was injected into the tail vein of male Wistar rats (180-250 g) on alternate days: 50 microg/kg (5 doses, N = 10), 100 microg/kg (2, 3, 4 and 5 doses, N = 49) or 150 microg/kg (1, 2, or 5 doses, N = 37). Weight and stool output were measured daily for each animal. One day after completing the vincristine treatment, the animals were fasted for 24 h, gavage-fed with a test meal and sacrificed 10 min later to measure gastric emptying (GE), GI transit and colon weight. Sensory peripheral neuropathy was evaluated by hot plate testing. Chronic vincristine treatments with total cumulative doses of at least 250 microg/kg significantly decreased GE by 31-59% and GI transit by 55-93%. The effect of 5 doses of vincristine (150 microg/kg) on GE did not persist for more than 1 week. Colon weight increased after 2 and 5 doses of vincristine (150 microg/kg). Fecal output decreased up to 48 h after the fifth dose of vincristine (150 microg/kg). Vincristine decreased the heat pain threshold 1 day after 5 doses of 50-100 microg/kg or after 3-5 doses of 150 microg/kg. This effect lasted for at least 2 weeks after the fifth dose. Chronic intravenous vincristine treatment delayed GE and GI transit of liquid. This effect correlated with the peak increase in colon weight but not with the pain threshold changes.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Autonomic Nervous System Diseases/chemically induced , Gastric Emptying/drug effects , Gastrointestinal Transit/drug effects , Vincristine/pharmacology , Animals , Antineoplastic Agents, Phytogenic/administration & dosage , Dose-Response Relationship, Drug , Male , Organ Size/drug effects , Pain Measurement/drug effects , Rats , Rats, Wistar , Time Factors , Vincristine/administration & dosage
4.
Rev Inst Med Trop Sao Paulo ; 42(3): 137-9, 2000.
Article in English | MEDLINE | ID: mdl-10887372

ABSTRACT

A total of 40 strains of the B. fragilis group was isolated from clinical specimens in two hospital centers in Fortaleza from 1993 to 1997. The most frequently isolated species was Bacteroides fragilis (19 strains) and most isolates came from intra-abdominal and wound infections. The susceptibility profile was traced for cefoxitin, cefoperazone and ticarcillin-clavulanate by using the agar dilution reference method. All isolates were susceptible to ticarcillin-clavulanate (128/2 microg/ml). Resistance rates of 15 and 70% were detected to cefoxitin (64 microg/ml) and cefoperazone (64 microg/ml), respectively. Such regional results permit a better orientation in choosing this group of antibiotics for prophylaxis and therapy especially in relation to cefoxitin, which is frequently used in the hospital centers studied.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteroides fragilis/drug effects , Cefoperazone/pharmacology , Cefoxitin/pharmacology , Clavulanic Acid/pharmacology , Ticarcillin/pharmacology , beta-Lactam Resistance
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