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1.
Braz Oral Res ; 32: e25, 2018 Mar 15.
Article in English | MEDLINE | ID: mdl-29561951

ABSTRACT

The objective of this study was to evaluate the in vitro effects, including surface morphological characteristics and chemical elemental properties, of different mouthwash formulations on enamel and dental restorative materials, simulating up to 6 months of daily use. Human enamel samples, hydroxyapatite, composite resin, and ceramic surfaces were exposed to 3 different mouthwashes according to label directions - Listerine® Cool Mint®, Listerine® Total Care, and Listerine® Whitening - versus control (hydroalcohol solution) to simulate daily use for up to 6 months. The samples were analyzed using scanning electron microscopy (SEM), infrared spectrophotometry (µ-Fourier transform infrared microscopy), energy-dispersive X-ray (EDX) spectroscopy, and color analysis before and after exposure. No relevant changes were observed in the morphological characteristics of the surfaces using SEM techniques. The physical and chemical aspects of the enamel surfaces were evaluated using mid-infrared spectroscopy, and EDX fluorescence was used to evaluate the elemental aspects of each surface. There was no variation in the relative concentrations of calcium and phosphorus in enamel, silicon and barium in composite resin, and silicon and aluminum in the ceramic material before and after treatment. No relevant changes were detected in the biochemical and color properties of any specimen, except with Listerine® Whitening mouthwash, which demonstrated a whitening effect on enamel surfaces. Long-term exposure to low pH, alcohol-containing, and peroxide-containing mouthwash formulations caused no ultra-structural or chemical elemental changes in human enamel or dental restorative materials in vitro.


Subject(s)
Ceramics , Composite Resins , Dental Enamel/drug effects , Durapatite , Ethanol/chemistry , Ethanol/pharmacology , Mouthwashes/chemistry , Mouthwashes/pharmacology , Salicylates , Terpenes , Color , Colorimetry , Drug Combinations , Humans , Hydrogen Peroxide/chemistry , Immersion , Materials Testing , Microscopy, Electron, Scanning , Oils, Volatile/chemistry , Reference Values , Reproducibility of Results , Spectrometry, X-Ray Emission , Spectroscopy, Fourier Transform Infrared , Surface Properties/drug effects , Time Factors
2.
Braz. oral res. (Online) ; 32: e25, 2018. tab, graf
Article in English | LILACS | ID: biblio-889489

ABSTRACT

Abstract The objective of this study was to evaluate the in vitro effects, including surface morphological characteristics and chemical elemental properties, of different mouthwash formulations on enamel and dental restorative materials, simulating up to 6 months of daily use. Human enamel samples, hydroxyapatite, composite resin, and ceramic surfaces were exposed to 3 different mouthwashes according to label directions — Listerine® Cool Mint®, Listerine® Total Care, and Listerine® Whitening — versus control (hydroalcohol solution) to simulate daily use for up to 6 months. The samples were analyzed using scanning electron microscopy (SEM), infrared spectrophotometry (µ-Fourier transform infrared microscopy), energy-dispersive X-ray (EDX) spectroscopy, and color analysis before and after exposure. No relevant changes were observed in the morphological characteristics of the surfaces using SEM techniques. The physical and chemical aspects of the enamel surfaces were evaluated using mid-infrared spectroscopy, and EDX fluorescence was used to evaluate the elemental aspects of each surface. There was no variation in the relative concentrations of calcium and phosphorus in enamel, silicon and barium in composite resin, and silicon and aluminum in the ceramic material before and after treatment. No relevant changes were detected in the biochemical and color properties of any specimen, except with Listerine® Whitening mouthwash, which demonstrated a whitening effect on enamel surfaces. Long-term exposure to low pH, alcohol-containing, and peroxide-containing mouthwash formulations caused no ultra-structural or chemical elemental changes in human enamel or dental restorative materials in vitro.


Subject(s)
Humans , Ceramics , Composite Resins , Dental Enamel/drug effects , Durapatite , Ethanol/chemistry , Ethanol/pharmacology , Mouthwashes/chemistry , Mouthwashes/pharmacology , Salicylates , Terpenes , Color , Colorimetry , Drug Combinations , Hydrogen Peroxide/chemistry , Immersion , Materials Testing , Microscopy, Electron, Scanning , Oils, Volatile/chemistry , Reference Values , Reproducibility of Results , Spectrometry, X-Ray Emission , Spectroscopy, Fourier Transform Infrared , Surface Properties/drug effects , Time Factors
3.
Cien Saude Colet ; 20(1): 85-94, 2015 Jan.
Article in Portuguese | MEDLINE | ID: mdl-25650601

ABSTRACT

The scope of this study is to identify the prevalence of access to information about how to prevent oral problems among schoolchildren in the public school network, as well as the factors associated with such access. This is a cross-sectional and analytical study conducted among 12-year-old schoolchildren in a Brazilian municipality with a large population. The examinations were performed by 24 trained dentists and calibrated with the aid of 24 recorders. Data collection occurred in 36 public schools selected from the 89 public schools of the city. Descriptive, univariate and multiple analyses were conducted. Of the 2510 schoolchildren included in the study, 2211 reported having received information about how to prevent oral problems. Access to such information was greater among those who used private dental services; and lower among those who used the service for treatment, who evaluated the service as regular or bad/awful. The latter use toothbrush only or toothbrush and tongue scrubbing as a means of oral hygiene and who reported not being satisfied with the appearance of their teeth. The conclusion drawn is that the majority of schoolchildren had access to information about how to prevent oral problems, though access was associated with the characteristics of health services, health behavior and outcomes.


Subject(s)
Access to Information , Mouth Diseases/prevention & control , Brazil , Child , Cross-Sectional Studies , Female , Humans , Male , Public Sector , Schools
4.
Ciênc. Saúde Colet. (Impr.) ; 20(1): 85-94, jan. 2015. tab
Article in Portuguese | LILACS | ID: lil-733143

ABSTRACT

O presente estudo propõe-se a identificar a prevalência do acesso a informações sobre como evitar problemas bucais entre escolares da rede pública de ensino, assim como os fatores associados a este acesso. Trata-se de um estudo transversal e analítico conduzido entre escolares de 12 anos de idade de um município brasileiro de grande porte populacional. Os exames foram realizados por 24 cirurgiões-dentistas treinados e calibrados com auxilio de 24 anotadores. A coleta de dados ocorreu em 36 escolas sorteadas das 89 escolas públicas do município. Foram conduzidas análises descritivas, univariadas e múltiplas. Dos 2510 escolares incluídos no estudo, 2211 relataram já ter recebido informações sobre como evitar problemas bucais. O acesso a tais informações foi maior entre os que utilizaram serviços odontológicos privado/convênio; e menor entre aqueles que utilizaram o serviço para tratamento, os que avaliaram o serviço como regular ou ruim/péssimo, os que utilizam como meio de higiene bucal somente escova dente/escova dente e higienização a língua e os que relataram não estarem satisfeitos com a aparência de seus dentes. Conclui-se que a maioria dos escolares teve acesso a informações sobre como evitar problemas bucais, o qual esteve associado a características dos serviços de saúde, comportamentos e desfechos de saúde.


The scope of this study is to identify the prevalence of access to information about how to prevent oral problems among schoolchildren in the public school network, as well as the factors associated with such access. This is a cross-sectional and analytical study conducted among 12-year-old schoolchildren in a Brazilian municipality with a large population. The examinations were performed by 24 trained dentists and calibrated with the aid of 24 recorders. Data collection occurred in 36 public schools selected from the 89 public schools of the city. Descriptive, univariate and multiple analyses were conducted. Of the 2510 schoolchildren included in the study, 2211 reported having received information about how to prevent oral problems. Access to such information was greater among those who used private dental services; and lower among those who used the service for treatment, who evaluated the service as regular or bad/awful. The latter use toothbrush only or toothbrush and tongue scrubbing as a means of oral hygiene and who reported not being satisfied with the appearance of their teeth. The conclusion drawn is that the majority of schoolchildren had access to information about how to prevent oral problems, though access was associated with the characteristics of health services, health behavior and outcomes.


Subject(s)
Humans , Animals , Mice , Autoimmune Diseases/immunology , Autoimmunity/immunology , Mast Cells/immunology , Multiple Sclerosis/immunology , Central Nervous System/immunology , Dendritic Cells/immunology , Self Tolerance , T-Lymphocytes/immunology
5.
J Craniofac Surg ; 23(3): 702-5, 2012 May.
Article in English | MEDLINE | ID: mdl-22565877

ABSTRACT

The use of high-power lasers has facilitated and improved human papillomavirus (HPV) treatment protocols and has also become very popular in recent years. This application has been more frequently used in hospitals, especially in gynecology. The present study aimed to evaluate the effects of high-power diode laser to remove oral lesions caused by HPV and the consequent effects on virus load following the wound tissue healing process compared with one of the most conventional surgical techniques involving electrocautery. Surgeries were performed on 5 patients who had 2 distinct lesions caused by HPV. All patients were submitted to both electrocautery and high-power diode laser. Following a 20-day period, when the area was healed, sample material was collected through curettage for virus load quantitative analysis.Observation verified the presence of virus in all the samples; however, surgeries performed with the laser also revealed a significant reduction in virus load per cell compared with those performed with electrocautery. The ease when handling the diode laser, because of the flexibility of its fibers and precision of its energy delivery system, provides high-accuracy surgery, which facilitates the treatment of large and/or multifocal lesions. The use of high-power diode laser is more effective in treatment protocols of lesions caused by HPV.


Subject(s)
Electrocoagulation , Laser Therapy/methods , Lasers, Semiconductor/therapeutic use , Mouth Diseases/surgery , Mouth Diseases/virology , Papillomavirus Infections/surgery , Adult , Female , Humans , Laser Therapy/instrumentation , Male , Middle Aged , Polymerase Chain Reaction , Statistics, Nonparametric , Treatment Outcome , Viral Load , Wound Healing
7.
Lasers Med Sci ; 25(3): 391-5, 2010 May.
Article in English | MEDLINE | ID: mdl-19585079

ABSTRACT

The ability of Staphylococcus aureus to develop multidrug resistance is well documented, and the antibiotic resistance showed by an increasing number of bacteria has shown the need for alternative therapies to treat infections, photodynamic therapy (PDT) being a potential candidate. The aim of this study was to determine the effect of photodynamic therapy as a light-based bactericidal modality to eliminate Staphylococcus aureus. The study investigated a technique based on a combination of light and a photosensitizer that is capable of producing oxidative species to induce a cytotoxic effect. A Staphylococcus aureus suspension was exposed to a light emitting diode (LED) emitting at 628 nm, 14.6 mW/cm(2), and energy density of 20 J/cm(2), 40 J/cm(2), or 60 J/cm(2) in the presence of different porphyrin concentrations (Photogem). Three drug concentrations were employed: 12 microl/ml, 25 microl/ml, and 50 microl/ml. The treatment response was evaluated by the number of bacterial colony forming units (CFU) after light exposure. The results indicated that exposure to 60 J/cm(2) eliminated 100% (10 log(10) scales) of bacteria, on average. The best PDT response rate to eliminate Staphylococcus aureus was achieved with exposure to LED light in combination with the photosensitizer at concentrations ranging from 25 microl/ml to 50 microl/ml. These data suggest that PDT has the potential to eliminate Staphylococcus aureus in suspension and indicates the necessary drug concentration and light fluency.


Subject(s)
Hematoporphyrins/pharmacology , Photochemotherapy , Photosensitizing Agents/pharmacology , Staphylococcus aureus/drug effects , Colony Count, Microbial , Humans , In Vitro Techniques , Lasers, Semiconductor , Staphylococcal Infections/drug therapy , Staphylococcus aureus/radiation effects
9.
Photomed Laser Surg ; 27(6): 937-41, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19715431

ABSTRACT

OBJECTIVE: The aim of this in vitro study was to evaluate the effects of diode laser irradiation on human dental enamel treated by bleaching and acidulated phosphate fluoride gels (APF) according to microhardness analysis. BACKGROUND DATA: The high interest of patients for aesthetics in cosmetic dentistry has led to new bleaching materials and the development of new techniques. MATERIALS AND METHODS: Sixty embedded, flattened human third molar enamel fragments were randomly assigned to three groups (n=20). Group 1 received topical application of 1.23% APF photoactivated using a diode laser. Group 2 received three applications of 35% hydrogen peroxide for 5 min and was photoactivated with laser for 30 s in each application. Group 3 treated the same as group 2 plus a 1.23% APF application for 1 min after bleaching followed by 30 s of laser irradiation. Microhardness analyses were performed before and after all the treatments. RESULTS: Analysis of variance followed by Tukey and Student statistical t-tests (p<0.05) showed significantly higher microhardness values for group 1 after treatment. No significant differences were shown before and after treatments for groups 2 and 3. CONCLUSION: No change was observed in enamel microhardness after treatment with hydrogen peroxide gel photoactivated using diode laser with or without APF. There was an increase in microhardness when enamel was treated only with APF photoactivated using a laser.


Subject(s)
Acidulated Phosphate Fluoride/pharmacology , Dental Enamel/drug effects , Dental Enamel/radiation effects , Lasers, Semiconductor , Tooth Bleaching/methods , Analysis of Variance , Hardness , Humans , Hydrogen Peroxide/pharmacology , Molar , Surface Properties
10.
Rev. odonto ciênc ; 22(55): 36-41, jan.-mar. 2007. ilus, tab, graf
Article in Portuguese | LILACS, BBO - Dentistry | ID: lil-462895

ABSTRACT

O objetivo desta pesquisa foi o de avaliar o comportamento clínico do laser Nd:YAG em comparação ao de um selante com carga, durante o período de 12 meses, observando; a pigmentação do sulco, incidência de cárie e fratura ou perda do selante. Foram selecionados 62 primeiros e segundos premolares hígidos de pacientes na faixa etária de 12 a 15 anos, de uma escola da rede pública de São José dos Campos. Nos 31 primeiros premolares foi aplicado o laser Nd:YAG, Pulse Máster 600 IQ em toda a extensão do sulco oclusal por 30 segundos utilizando 60 mJ, 10 HZ, 0,6W. Nos 31 segundos premolares foi realizado o selamento dos sulcos oclusais com o selante Fluorshield. Após 6 meses observou-se que dos casos selados com Fluorshield 19,35% apresentaram perda e/ou fratura do selante e em 16,66% os sulcos apresentaram cárie incipiente. Já nos casos em que foi aplicado o laser Nd:YAG 6,45% apresentaram início de lesão de cárie. Com 12 meses de avaliação tanto com selante Fluorshield como com o laser Nd:YAG observou-se o aparecimento de sulcos pigmentados em 12, 90% dos casos e uma incidência de 9,67% de lesões incipientes de cárie. Utilizando-se o selante Fluorshield houve queda do material em 25,80% e fratura em 3,22% dos casos. Concluímos que após 12 meses de avaliação o laser Nd:YAG e o selante Fluorshield apresentaram comportamento clínico semelhante, diante das mesmas condições bucais, sendo que a porcentagem de aparecimento de lesão inicial de cárie foi igual para os dois métodos de prevenção utilizados.


Subject(s)
Humans , Male , Female , Child , Adolescent , Lasers/therapeutic use , Preventive Dentistry , Pit and Fissure Sealants
11.
Rev. Assoc. Paul. Cir. Dent ; 56(5): 387-set.-out. 2002. ilus
Article in Portuguese | LILACS, BBO - Dentistry | ID: biblio-872361

ABSTRACT

A estética na odontologia está cada vez mais em evidência. Os pacientes estão mais exigentes com relação à estética das restaurações. O desejo por "dentes mais brancos" está motivando a procura de profissionais para a realização do clareamento dental


Subject(s)
Lasers , Tooth Bleaching , Esthetics, Dental
14.
Rev. odontol. UNESP ; 24(1): 61-8, jan.-jun. 1995. tab
Article in Portuguese | LILACS, BBO - Dentistry | ID: lil-160013

ABSTRACT

A finalidade desta pesquisa foi avaliar a efetividade de dois adesivos (ARMD sem primer e Prisma Bond 3 com primer) nos selamento de restauraçöes em cavidades de Classe II, MOD, com parede gengival em cemento e, ainda, com e sem o pré-tratamento da dentina, utilizando a soluçäo de ácido maléico a 25 por cento. Após a realizaçäo das restauraçöes, os dentes foram avaliados quanto à intensidade de uniäo à estrutura dental por infiltraçäo marginal, pela penetraçäo do corante Rodamina, durante a ciclagem térmica. Pelos resultados obtidos verificamos que o pré-tratamento da dentina com a soluçäo de ácido maléico pouco melhorou a eficiência do adesivo em diminuir a infiltraçäo marginal, näo apresentando diferença estatisticamente significante. Os melhores resultados foram obtidos nos grupos que receberam adesivos com primer, nos quais a infiltraçäo marginal foi estatisticamente nula


Subject(s)
Humans , Adult , Adhesives , Composite Resins/therapeutic use , Dental Materials/therapeutic use , Dental Leakage/therapy , Dental Materials , Dental Restoration, Permanent/methods
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