ABSTRACT
The genus Arthrospira comprises a group of filamentous multicellular cyanobacteria and can be used for animal feed and human food. Solid state fermentation or cultivation (SSF) involves the use of a culture medium composed of solid material with given moisture content. No studies have been published about the cultivation of microalgae or cyanobacteria on solid medium. Furthermore, although sugar-cane bagasse is used as source of energy in alcohol distilleries in Brazil, the excess could be a support to photosynthetic microorganism growth. The experimental design methodology was used to evaluate the protein production by Arthrospira platensis under SSF using sugarcane bagasse as support, taking into account the moisture content of the medium, light intensity and inoculum concentration. Moisture was found to have a strong influence on the performance of the process. The best conditions were: moisture of 98.8%; inoculum concentration of 0.15 g biomass·kg wet culture medium-1 and light intensity of 6.0 klx.
ABSTRACT
This paper presents a method to estimate the biomass of Spirulina cultivated on solid medium with sugarcane bagasse as a support, in view of the difficulty in determining biomass concentrations in bioprocesses, particularly those conducted in semi-solid or solid media. The genus Spirulina of the family Oscillatoriaceae comprises the group of multicellular filamentous cyanobacteria (blue-green microalgae). Spirulina is used as fish feed in aquaculture, as a food supplement, a source of vitamins, pigments, antioxidants and fatty acids. Therefore, its growth parameters are extremely important in studies of the development and optimization of bioprocesses. For studies of biomass growth, Spirulina platensis was cultured on solid medium using sugarcane bagasse as a support. The biomass thus produced was estimated by determining the protein content of the material grown during the process, based on the ratio of dry weight to protein content obtained in the surface growth experiments. The protein content of the biomass grown in Erlenmeyer flasks on surface medium was examined daily to check the influence of culture time on the protein content of the biomass. The biomass showed an average protein content of 42.2%. This methodology enabled the concentration of biomass adhering to the sugarcane bagasse to be estimated from the indirect measurement of the protein content associated with cell growth.
Subject(s)
Bacterial Proteins/analysis , Biomass , Culture Media/chemistry , Microbiological Techniques/methods , Spirulina/chemistry , Spirulina/growth & development , Cellulose , SaccharumABSTRACT
This paper presents a method to estimate the biomass of Spirulina cultivated on solid medium with sugarcane bagasse as a support, in view of the difficulty in determining biomass concentrations in bioprocesses, particularly those conducted in semi-solid or solid media. The genus Spirulina of the family Oscillatoriaceae comprises the group of multicellular filamentous cyanobacteria (blue-green microalgae). Spirulina is used as fish feed in aquaculture, as a food supplement, a source of vitamins, pigments, antioxidants and fatty acids. Therefore, its growth parameters are extremely important in studies of the development and optimization of bioprocesses. For studies of biomass growth, Spirulina platensis was cultured on solid medium using sugarcane bagasse as a support. The biomass thus produced was estimated by determining the protein content of the material grown during the process, based on the ratio of dry weight to protein content obtained in the surface growth experiments. The protein content of the biomass grown in Erlenmeyer flasks on surface medium was examined daily to check the influence of culture time on the protein content of the biomass. The biomass showed an average protein content of 42.2%. This methodology enabled the concentration of biomass adhering to the sugarcane bagasse to be estimated from the indirect measurement of the protein content associated with cell growth.
Subject(s)
Biomass , Bacterial Proteins/analysis , Culture Media/chemistry , Microbiological Techniques/methods , Spirulina/chemistry , Spirulina/growth & development , Cellulose , SaccharumABSTRACT
A sociedade tem atualmente dado cada vez mais importância à alimentação, como forma de prevenção de doenças e fonte de energia saudável. Não se deve esquecer, no entanto, a higiene na manipulação dos alimentos, o que torna imprescindível a aplicação de boas práticas nas cozinhas, através da avaliação e orientação das pessoas quanto às principais medidas de higiene a serem aplicadas. Visando isso, o presente trabalho fez a análise de sete cozinhas de escolas da rede pública de ensino, utilizando como metodologia a pesquisa em livros e periódicos que tratam do assunto e a visita às cozinhas das escolas selecionadas, aplicando-se um check-list para avaliar as condições higiênico-sanitárias das mesmas. O levantamento e a análise dos dados levam à reafirmação do papel do nutricionista nas cozinhas.
Subject(s)
School Feeding/standards , Food Hygiene , Good Manipulation PracticesABSTRACT
O presente trabalho refere-se ao desenvolvimento de um processo de produção de Spirulina platensis através do cultivo em estado sólido, utilizando-se bagaço de cana-de-açúcar como suporte para o crescimento celular. Foram realizados experimentos, primeiramente em frascos de Erlenmeyer, de acordo com um planejamento experimental que levou em conta três variáveis: umidade inicial do meio de cultivo (U, variando de 92,3 a 95,8 por cento), iluminância (I, variando de 2,3 a 9,7Klux) e concentração de inóculo (C, variando de 0,02 a 0,32g biomassa.Kgîû de meio de cultura úmido). Foi desenvolvido um reator tipo bandejas para a produção de S. platensis, estudando a influência da altura da camada do meio de cultivo...
