ABSTRACT
Based upon synthetic and biochemical results, a novel and potent tacrine analogue and heterobivalent analogues of tacrine, were designed. The role played by the amino groups of homo- and heterobivalent ligands in the interaction with the peripheral and catalytic sites of AChE and BuChE were investigated. The syntheses of these materials together with the results of AChE/BuChE inhibition assays are detailed.
Subject(s)
Acetylcholinesterase/metabolism , Butyrylcholinesterase/metabolism , Tacrine/metabolism , Acetylcholinesterase/chemistry , Butyrylcholinesterase/chemistry , Catalytic Domain , Ligands , Tacrine/chemistryABSTRACT
A large series of 2-aryl(heteroaryl)-2,5-dihydropyrazolo[4,3-c]quinolin-3(3H)-ones (PQ, 106 compounds), carrying appropriate substituents at the quinoline and N2-phenyl rings, were designed, prepared and tested as central benzodiazepine receptor ligands. Compounds with an affinity significantly higher than the parent compound CGS-8216 were obtained, the most active ligand showing a pIC50 = 10.35. Hansch and comparative molecular field analyses gave coherent results suggesting the main structural requirements of high receptor binding affinity. The possible formation of a three-centred hydrogen bond (HB) at the HB donor site H2, as a key interaction for high receptor binding affinity, was assessed by the calculation and comparison of the molecular electrostatic potentials of a series of selected ligands.
Subject(s)
Quantitative Structure-Activity Relationship , Receptors, GABA-A/metabolism , Animals , Binding, Competitive , Cerebral Cortex/metabolism , GABA Agonists/chemical synthesis , GABA Agonists/chemistry , GABA Agonists/metabolism , GABA Antagonists/chemical synthesis , GABA Antagonists/chemistry , GABA Antagonists/metabolism , Inhibitory Concentration 50 , Ligands , Magnetic Resonance Spectroscopy , Male , Models, Molecular , Protein Binding , Pyrazoles/metabolism , Quinolones/chemical synthesis , Quinolones/chemistry , Quinolones/metabolism , Rats , Rats, Sprague-Dawley , Receptors, GABA-A/chemistry , Static ElectricityABSTRACT
The synthesis of a series of 1,2,4-triazolo[4,3-a]quinoline derivatives is described; their structures were assigned by 1H NMR and analytical data. The new compounds were tested in vivo for their antiinflammatory and analgesic activities, as well as for their ulcerogenic action. Some of the tested triazoles showed an analgesic activity in the acetic acid writhing test and antiinflammatory properties on carrageenan paw edema assay.
Subject(s)
Analgesics/chemical synthesis , Anti-Inflammatory Agents/chemical synthesis , Isoquinolines/pharmacology , Triazoles/pharmacology , Analgesics/pharmacology , Analgesics/toxicity , Animals , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/toxicity , Edema/drug therapy , Edema/prevention & control , Female , Isoquinolines/chemical synthesis , Isoquinolines/toxicity , Magnetic Resonance Spectroscopy , Male , Mice , Molecular Structure , Pain/drug therapy , Pain/prevention & control , Structure-Activity Relationship , Triazoles/chemical synthesis , Triazoles/toxicityABSTRACT
A large series of 2-aryl(heteroaryl)-2,5-dihydropyrazolo[4,3-c]quinolin- 3-(3H)-ones, carrying appropriate substituents at the quinoline and N2-phenyl rings, were prepared and tested as central benzodiazepine receptor ligands. Results from structure-affinity relationship studies were in full agreement with previously proposed pharmacophore models and, in addition, quantitative structure-activity analysis gave further significant insight into the main molecular determinants of high benzodiazepine receptor affinity. The intrinsic activity of some active ligands was also determined and preliminary discussed.
Subject(s)
GABA Agonists/chemistry , GABA Antagonists/chemistry , Pyrazoles/chemistry , Quinolones/chemistry , Receptors, GABA-A/chemistry , Animals , Anti-Anxiety Agents/metabolism , Cerebral Cortex/metabolism , Culture Techniques , Flunitrazepam/metabolism , GABA Agonists/metabolism , GABA Antagonists/metabolism , Ligands , Magnetic Resonance Spectroscopy , Male , Pyrazoles/metabolism , Quinolones/metabolism , Rats , Rats, Sprague-Dawley , Spectrophotometry, Infrared , Structure-Activity RelationshipABSTRACT
A number of pyruvic acid and methylpyruvate alpha-(N)-heterocyclic hydrazones has been synthesized. Bis-heterocyclic hydrazones were obtained from reaction with pyruvic carboxaldehyde. Some complexes of Ni(II) were prepared and characterized as neutral complexes. All these compounds have been evaluated for cytotoxicity against P388 and HL-60 leukemia.
Subject(s)
Antineoplastic Agents/chemical synthesis , Chelating Agents/chemical synthesis , Hydrazones/chemical synthesis , Animals , Antineoplastic Agents/pharmacology , Chelating Agents/pharmacology , Drug Screening Assays, Antitumor , HL-60 Cells , Humans , Hydrazones/pharmacology , Leukemia P388/drug therapy , Ligands , Magnetic Resonance Spectroscopy , Mice , Spectrophotometry, Infrared , Tumor Cells, CulturedABSTRACT
Several 1-[quinolyl(4)]-1,2,3-triazoles were synthesized by 1,3-dipolar cycloaddition of 4-azidoquinolines with activated methylene compounds. The synthesized compounds, tested for antiinflammatory and analgesic activities, resulted moderately active as antiinflammatories, but with a very interesting analgesic activity, sometimes higher than that of indomethacin, used as reference drug. Some of the triazole derivatives were evaluated also as antimicrobial, but none of them exhibited activity.
Subject(s)
Analgesics/chemical synthesis , Anti-Inflammatory Agents, Non-Steroidal/chemical synthesis , Triazoles/chemical synthesis , Analgesics/pharmacology , Animals , Anti-Infective Agents/chemical synthesis , Anti-Infective Agents/pharmacology , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Male , Rats , Rats, Wistar , Structure-Activity Relationship , Triazoles/pharmacologyABSTRACT
The synthesis of new 1-[quinolyl(4)]-1,2,3-triazoles is reported. These have been obtained by reacting 4-azidoquinolines with ethyl p-nitrobenzoylacetate. The synthesized compounds, tested for antiinflammatory and analgesic activities, results moderately active as antiinflammatories, but with a very interesting analgesic activity, sometimes higher than that of indomethacin, used as reference drug.
Subject(s)
Analgesics/chemical synthesis , Anti-Inflammatory Agents, Non-Steroidal/chemical synthesis , Triazoles/chemical synthesis , Analgesics/adverse effects , Analgesics/pharmacology , Animals , Anti-Inflammatory Agents, Non-Steroidal/adverse effects , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Magnetic Resonance Spectroscopy , Male , Rats , Rats, Wistar , Spectrophotometry, Ultraviolet , Stomach Ulcer/chemically induced , Stomach Ulcer/prevention & control , Triazoles/adverse effects , Triazoles/pharmacologyABSTRACT
Two new series of 2-arylpyrazolo[4,3-c]quinolin-3-ones (6,8-difluoro- and 7,9-dichloro-derivatives) have been synthesized and tested for their ability to displace [3H]flunitrazepam from rat brain membranes. Several compounds possess comparable and sometimes higher affinity for central benzodiazepine receptors than that of diazepam. Some selected compounds were also tested in vivo in the anti-pentylenetetrazol test; some anticonvulsant activity resulted for the 6,8-difluoroderivatives only.
Subject(s)
Anticonvulsants/chemical synthesis , Pyrazoles/chemical synthesis , Quinolines/chemical synthesis , Receptors, GABA-A/metabolism , Animals , Anticonvulsants/chemistry , Anticonvulsants/pharmacology , Binding Sites , Binding, Competitive , Female , Flunitrazepam/metabolism , Mice , Pentylenetetrazole/antagonists & inhibitors , Pyrazoles/chemistry , Pyrazoles/pharmacology , Quinolines/chemistry , Quinolines/pharmacology , Rats , Receptors, GABA-A/drug effects , Structure-Activity RelationshipABSTRACT
The synthesis of new halogenated series of 4-anilinoquinoline-3-carboxylic acids, N-[3-carboxyquinolyl(4)]anthranilic acids and their corresponding esters is reported. These have been obtained by reacting 4-chloro-3-carbethoxy-quinolines with variously substituted anilines and methyl anthranilate respectively. The synthesized compounds were tested for antiinflammatory and analgesic activities; some of them showed a good analgesic activity, sometimes higher than that of indomethacin, used as reference drug.
Subject(s)
Aniline Compounds/chemical synthesis , Anti-Inflammatory Agents, Non-Steroidal/chemical synthesis , Quinolines/chemical synthesis , ortho-Aminobenzoates/chemical synthesis , Aniline Compounds/pharmacology , Aniline Compounds/toxicity , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Anti-Inflammatory Agents, Non-Steroidal/toxicity , Carrageenan , Chemical Phenomena , Chemistry, Physical , Edema/chemically induced , Edema/prevention & control , In Vitro Techniques , Indomethacin/pharmacology , Male , Pain Measurement/drug effects , Platelet Aggregation/drug effects , Quinolines/pharmacology , Quinolines/toxicity , Rabbits , Rats , Rats, Wistar , Stomach Ulcer/chemically induced , ortho-Aminobenzoates/pharmacology , ortho-Aminobenzoates/toxicityABSTRACT
Synthesis and pharmacological evaluation of a series of 4-quinolylazide derivatives are reported. These were screened against P388 lymphocitic leukemia in mice, but they resulted inactive. All the compounds were also tested for their antimicrobial activity against gram-positive, gram-negative strains and fungi; only three derivatives exhibited poor activity.
Subject(s)
Anti-Infective Agents/chemical synthesis , Antineoplastic Agents/chemical synthesis , Azides/chemical synthesis , Quinolines/chemical synthesis , Animals , Anti-Bacterial Agents , Anti-Infective Agents/pharmacology , Antineoplastic Agents/pharmacology , Azides/pharmacology , Bacteria/drug effects , Chemical Phenomena , Chemistry, Physical , Fungi/drug effects , Leukemia P388/drug therapy , Mice , Mice, Inbred Strains , Microbial Sensitivity Tests , Quinolines/pharmacologyABSTRACT
A series of 2-arylpyrazolo[4,3-c] quinolin-3-one derivatives, bearing different substituents in the two aromatic rings, were prepared and tested for their ability to displace [3H] flunitrazepam from rat brain membranes. Some compounds have shown an affinity for receptors comparable and sometimes higher than that of CGS series.
Subject(s)
GABA-A Receptor Antagonists , Pyrazoles/chemical synthesis , Quinolines/chemical synthesis , Animals , Binding, Competitive/drug effects , Brain/metabolism , Flunitrazepam/metabolism , In Vitro Techniques , Pyrazoles/pharmacology , Quinolines/pharmacology , Rats , Synaptosomes/drug effects , Synaptosomes/metabolismABSTRACT
The inhibitory activity of a series of 2- and 4-quinolinehydrazones on retroviral reverse transcriptase has been studied on enzymes from M-MuLV, RAV-2, and on a crude lysate of HIV-1, assuming the first two enzymes as potential models of the third. The highest activity is mainly found in lipophilic, water soluble 4-quinolinehydrazones. The inhibitory activity of these compounds decreases in changing from the M-MuLV to the RAV-2, and HIV-1 enzymes, in this order.
Subject(s)
Hydrazones/chemical synthesis , Quinolines/chemical synthesis , Retroviridae/enzymology , Reverse Transcriptase Inhibitors , Cell Survival/drug effects , Chemical Phenomena , Chemistry, Physical , Hydrazones/pharmacology , Quinolines/pharmacologyABSTRACT
New 4-anilinoquinoline-3-carboxylic acids, N-[3-carboxyquinolyl (4)]anthranilic acids and their corresponding esters were synthetized by reacting 4-chloro-3-carbethoxyquinolines with substituted anilines and methyl anthranilate respectively. All the compounds were tested for antiinflammatory and analgesic activities. Some derivatives showed a significant antiinflammatory activity comparable to that of indomethacin.
Subject(s)
Analgesics , Anti-Inflammatory Agents, Non-Steroidal , Quinolines/pharmacology , Animals , Carrageenan , Chemical Phenomena , Chemistry , Edema/chemically induced , Edema/drug therapy , Male , Pain/drug therapy , Pain/physiopathology , Quinolines/chemical synthesis , Rats , Rats, Inbred Strains , Reaction Time/drug effects , Sensory Thresholds/drug effectsABSTRACT
Tridentate chelating agents, as potential antitumor agents, were prepared by condensing 2-quinolylhydrazines, 2-pyridylhydrazine and 2-benzothiazolylhydrazine with pyridine-2-aldehyde, 6-methylpyridine-2-aldehyde, 2-acetylpyridine and 2-benzoylpyridine. All compounds were tested against Lymphocytic leukemia P388. The active pyridine-2-aldehyde-4-methyl-2-quinolylhydrazone [1-(4'-methyl-2'-quinolyl)-3-(2'-pyridyl)-1,2-diaza-2-propene] (I d) was also tested against other experimental tumors and proved inactive.
Subject(s)
Antineoplastic Agents , Chelating Agents , Animals , Antineoplastic Agents/administration & dosage , Antineoplastic Agents/chemical synthesis , Cell Line , Chelating Agents/administration & dosage , Chelating Agents/chemical synthesis , Chemical Phenomena , Chemistry , Female , Hydrazones/pharmacology , Leukemia P388/drug therapy , Male , MiceABSTRACT
Quinolinehydrazones prepared by condensation of hydrazinoquinolines with 1-phenyl-2,5-dimethyl-3-pyrrolcarboxaldehyde, 2-chloro-4-dimethylaminobenzaldehyde and 2,6-dichlorobenzaldehyde are described. All compounds were tested in vitro for antimicrobial activity, the results obtained are shown and discussed. The quinolinehydrazones of the 1-phenyl-2,5-dimethyl-3-pyrrolcarboxaldehyde were tested in vivo against Hymenolepis nana and Taenia taeniaeformis and proved inactive.
Subject(s)
Aminoquinolines/chemical synthesis , Anti-Bacterial Agents/chemical synthesis , Parasites/drug effects , Aminoquinolines/pharmacology , Animals , Antifungal Agents/chemical synthesis , Bacteria/drug effects , Chemical Phenomena , Chemistry , Hydrazones/chemical synthesis , Hydrazones/pharmacology , Hymenolepis/drug effects , Taenia/drug effectsABSTRACT
Benzaldehyde nitrogen mustard derivatives of hydrazinoquinolines, 9-hydrazinoacridine and 1,2,3,4-tetrahydro-9-hydrazinoacridine were synthesized; all compounds were tested against lymphocytic leukemia P388 and proved inactive.
Subject(s)
Antineoplastic Agents/chemical synthesis , Nitrogen Mustard Compounds/chemical synthesis , Quinolines/chemical synthesis , Animals , Antineoplastic Agents/pharmacology , Chemical Phenomena , Chemistry , Leukemia P388/drug therapy , Mice , Nitrogen Mustard Compounds/pharmacology , Quinolines/pharmacologyABSTRACT
The synthesis of some 1,3-dioxol[4,5-g]quinoline derivatives is described. The compounds show appreciable activity in vitro against some gram-positive bacteria and do not show any significant activity against P388 lymphocytic leukemia. Microbiological and antitumor results are presented.
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Antineoplastic Agents/chemical synthesis , Dioxoles/chemical synthesis , Quinolines/chemical synthesis , Animals , Anti-Bacterial Agents/pharmacology , Antineoplastic Agents/pharmacology , Antiprotozoal Agents/chemical synthesis , Antiprotozoal Agents/pharmacology , Bacteria/drug effects , Chemical Phenomena , Chemistry , Leukemia P388/drug therapy , MiceABSTRACT
One hundred and seven 4-quinolinehydrazones were synthesized and tested in vivo against the tapeworm Hymenolepis nana. Twenty-five derivatives showed significant cestocidal activity; structure-activity correlations were performed using Free-Wilson methodology. Two compounds, 2,6-dimethyl-4-[(3-pyridinylmethylene)hydrazino]quinoline and 2,6-dimethyl-4-[2p][(6-methyl)pyridinylmethylene]hydrazino)quinoline, predicted to be maximally active, effected 100% reduction of H. nana in mice at 200 mg/kg, p.o.