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Appl Environ Microbiol ; 62(9): 3466-9, 1996 Sep.
Article in English | MEDLINE | ID: mdl-8795239

ABSTRACT

Municipal sewage was screened for DNA encoding Shiga-like Toxin (SLT) II, a key protein involved in the virulence of enterohemorrhagic Escherichia coli. PCR analysis of sewage concentrates showed that DNA encoding SLT II was present in a single sample of untreated sewage and absent in all other samples tested (n = 6). Thermotolerant E. coli cultured from the sewage (n = 1,520) also tested negative for SLT II by colony hybridization.


Subject(s)
Bacterial Toxins/genetics , DNA, Bacterial/analysis , Escherichia coli/isolation & purification , Gastrointestinal Hemorrhage/microbiology , Sewage , Water Microbiology , Escherichia coli/pathogenicity , Shiga Toxin 1 , Shiga Toxin 2
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