1.
Appl Environ Microbiol
; 62(9): 3466-9, 1996 Sep.
Article
in English
| MEDLINE
| ID: mdl-8795239
ABSTRACT
Municipal sewage was screened for DNA encoding Shiga-like Toxin (SLT) II, a key protein involved in the virulence of enterohemorrhagic Escherichia coli. PCR analysis of sewage concentrates showed that DNA encoding SLT II was present in a single sample of untreated sewage and absent in all other samples tested (n = 6). Thermotolerant E. coli cultured from the sewage (n = 1,520) also tested negative for SLT II by colony hybridization.