Occurrence and quantification of culturable and viable but non-culturable (VBNC) pathogens in biofilm on different pipes from a metropolitan drinking water distribution system.

Waterborne pathogens have been found in biofilms grown in drinking water distribution system (DWDS). However, there is a lack of quantitative study on the culturability of pathogens in biofilms from metropolitan DWDS. In this study, we quantified culturable and viable but non-culturable (VBNC) Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa and Vibrio cholerae in biofilms collected from five kinds of pipes (galvanized steel pipe, steel pipe, stainless steel clad pipe, ductile cast iron pipe and polyethylene pipe) and associated drinking water at an actual chlorinated DWDS in use from China. The results of these comprehensive analyses revealed that pipe material is a significant factor influencing the culturability of pathogen and microbial communities. Network analysis of the culturable pathogens and 16S rRNA gene inferred potential interactions between microbiome and culturability of pathogens. Although the water quality met the Chinese national standard of drinking water, however, VBNC pathogens were detected in both biofilms and water from the DWDS. This investigation suggests that stainless steel clad pipe (SSCP) was a better choice for pathogen control compared with other metal pipes. To our knowledge, this is the first study on culturable and VBNC pathogens in biofilms of different pipe materials in metropolitan DWDS.

Change regularity of water quality parameters in leakage flow conditions and their relationship with iron release.

The long-term stagnation in metal water supply pipes, usually caused by intermittent consumption patterns, will cause significant iron release and water quality deterioration, especially at the terminus of pipelines. Another common phenomenon at the terminus of pipelines is leakage, which is considered helpful by allowing seepage of low-quality drinking water resulting from long-term stagnation. In this study, the effect of laminar flow on alleviating water quality deterioration under different leakage conditions was investigated, and the potential thresholds of the flow rate, which can affect the iron release process, were discussed. Based on a galvanized pipe and ductile cast iron pipe pilot platform, which was established at the terminus of pipelines, this research was carried out by setting a series of leakage rate gradients to analyze the influence of different leakage flow rates on iron release, as well as the relationship with chemical and biological parameters. The results showed that the water quality parameters were obviously influenced by the change in flow velocity. Water quality was gradually improved with an increase in flow velocity, but its change regularity reflected a diversity under different flow rates (p < 0.05). The iron release was remarkably correlated to the redox potential, dissolved oxygen, pH, iron-oxidized bacteria and sulfate-reducing bacteria. The cumulative total iron release (r = 0.587, p < 0.05) and total iron release rate (r = 0.71, p < 0.022) were significantly influenced by the changes in flow velocity. In short, they tended first to increase and then to decrease with an increasing flow velocity with the threshold as approximately 40% of the critical laminar flow velocity (1.16 × 10-3 m/s). For the pipes at the terminus of the drinking water distribution system, when the bulk water was at the critical laminar flow velocity, the concentration of total iron, the quantity and rate of total iron release remain relatively in an ideal and safe situation.