ABSTRACT
Sinperca chuatsi usually refuses eating dead bait or man-made feed due to its specific feeding habit. It was showed that some S. chuatsi could be induced to take lifeless bait with domestication gradually after long-term of cultivation. High cost, serious pollution, severe diseases, and other problems of S chuatsi cultivation can be solved effectively by selecting those liable to domestication via molecular marker and treating them with artificial feed on a large scale. Lipoprotein lipase, one of the key enzymes in lipoprotein metabolic processes, provides energy by catalyzing the oxidation of triglycerides lie in the core of chylomicron and very low density lipoprotein (VLDL) into glycerin and fatty acid, and saves energy. In order to search the distribution of the alleles and genotypes of lipoprotein lipase gene (lipoprotein LPL) gene in two feed habit domestication phenotype populations, SNP genetic polymorphisms in 6 and 7 introns and 6, 7, and 8 exons of LPL in S. chuatsi were analyzed by PCR product sequencing method. Three SNPs A25T, G26T, and C29G were detected, two of which were non-synonymous mutations. It was indicated by Chi-square test that the analysis between domesticated and undomesticated populations show no significant difference (P>0.05) between the three SNPs and feed habit. However, diplotype 2 in the two population showed significant difference (P<0.05) by assembling different genotypes of the three SNPs into five diplotypes. Polymorphic analysis of partial fragment of LPL genome in S. chuatsi was accomplished successfully. Therefore, LPL gene can be considered as a candidate gene and genetic marker for feed habit domestication in S. chuatsi, which lays the foundation for the work of molecular marker and selecting breed, which has extensive application prospect.
