Three new species of jumping spiders (Araneae, Salticidae) from Hunan, China.

Three new species of the genera Thiania C. L. Koch, 1846 and Yaginumaella Prószynski, 1979 are described and named as T.bamian sp. nov. (♂♀), T.flacata sp. nov. (♀) and Y.curvata sp. nov. (♂♀), from Hunan Province, China. Detailed descriptions, photos of somatic features and copulatory organs, as well as a distribution map are provided. Nucleotide data for the barcoding gene, cytochrome c oxidase subunit I (COI) of T.bamian sp. nov. (♂♀) and Y.curvata sp. nov. (♀) are provided.

The first record of Tricholathys Chamberlin & Ivie, 1935 (Araneae, Dictynidae) from China, with a new combination and descriptions of seven new species.

The genus Tricholathys, found for the first time in China, is surveyed and seven new species, T.burangensissp. nov. (♂♀, Thibet), T.chenzhenningisp. nov. (♂♀, Qinghai), T.hebeiensissp. nov. (♀, Hebei), T.lhunzeensissp. nov. (♂♀, Tibet), Tricholathysrelictoidessp. nov. (♂♀, Xinjiang), T.serratasp. nov. (♂♀, Tibet), and T.xizangensissp. nov. (♂♀, Tibet), are described. A new combination is proposed for Tricholathysalxa (Tang, 2011) comb. nov., ex. Argenna Thorell, 1870. Descriptions of all new species are provided, together with digital images, illustrations, and a distribution map. The DNA barcode information of four recently collected species is also provided.

Three new species of the genus Synagelides Strand, 1906 (Araneae, Salticidae) from Yunnan, China.

Three new species of Synagelides Strand, 1906 (Araneae, Salticidae) from Yunnan, China are described: S. furcatoides sp. nov. (♂♀), S. furcatus sp. nov. (♂) and S. montiformis sp. nov. (♂♀). Photographs of the habitus and copulatory organs as well as a distribution map are provided.

Two new species of the sac-spider genus Clubiona Latreille, 1804 (Araneae, Clubionidae) and the female of C. subcylindrata from China.

Two new species of the genus Clubiona Latreille, 1804 are described: C. tianpingshan sp. nov. (♂♀) and C. flammaforma sp. nov. (♂♀) from central and south China. The female of C. subcylindrata Wang et al., 2018 is described for the first time. Detailed descriptions, photographs of somatic features and copulatory organs, as well as a distribution map of these three species, are provided.

Five new species of Synagelides Strand, 1906 from China (Araneae, Salticidae).

Five new species of salticids were collected from China: Synagelidesemangou Liu, sp. nov. (♂, ♀) from Gansu province, and S.jinding Liu, sp. nov. (♂), S.serratus Liu, sp. nov. (♂, ♀), S.shuqiang Liu, sp. nov. (♂), and S.triangulatus Liu, sp. nov. (♀) from Jiangxi Province. All species are described and illustrated with photographs and SEM micrographs, and their distributions are also mapped.

Three new species of the genus Tetragnatha Latreille, 1804 (Araneae, Tetragnathidae) from China.

Three new species of Tetragnatha Latreille, 1804 are described from China: T.bifurcata Li & Liu, sp. nov. (♂♀) and T.tortilis Li & Liu, sp. nov. (♂♀) from Yunnan Province, and T.bimaculata Li & Liu, sp. nov. (♂♀) from Hubei and Hunan provinces. Detailed descriptions, photographs of somatic features and copulatory organs, and a distribution map of these three species are provided.

A survey of the genus Himalaphantes Tanasevitch, 1992 (Araneae, Linyphiidae) with description of three new species from Yunnan, China.

Three new species of Himalaphantes Tanasevitch, 1992 from Yunnan province, China, are described: H.arcuatus sp. nov. (♀), H.lingulatus sp. nov. (♂♀), and H.uncatus sp. nov. (♂♀). The diagnosis of the genus is clarified, and extended detailed descriptions, photographs of somatic features and copulatory organs, and a distribution map are provided.

Three new spider species of Araneus Clerck, 1757 (Araneae, Araneidae) from the Gaoligong Mountains of Yunnan, China.

Both sexes of three new species of the spider genus Araneus Clerck, 1757 are described and named as: A. breviscapus n. sp., A. floriformis n. sp. and A. pianmaensis n. sp. from the Yunnan province. Detailed descriptions, photos of somatic features and copulatory organs, as well as the map of collection localities are provided.

Three new species of the genus Asceua Thorell, 1887 (Araneae: Zodariidae) from Yunnan Province, China.

Three new species of the genus Asceua Thorell, 1887 are described and named as: A. calciformis sp. nov. (♂♀), A. digitata sp. nov. (♂) and A. forcipiformis sp. nov. (♂♀) from Yunnan province, China. Detailed descriptions, photos of somatic features and copulatory organs, as well as a distribution map are provided.

Four new species of the jumping spider genus Portia (Araneae, Salticidae) from China.

We diagnose and describe four new species of Portia Karsch, 1878 and describe for the first time the male of P.zhaoi Peng, Li & Chen, 2003 from China based on morphological characters. The females of Portiabawang sp. nov. have the narrowest epigyne orifice. The males of Portiaerlangping sp. nov. have the shortest embolus among all the species. The females of Portiafajing sp. nov. can be distinguished from other species by the anterior orifice margin, which is nearly parallel to the posterior orifice margin. The males of Portiaxishan sp. nov. can be identified by the tegular furrow which extends to form a membrane and by the tegular apophysis which is obscured; the females of Portiaxishan sp. nov. can be diagnosed by the slit-like epigynal orifice. The males of P.zhaoi have the longest embolus among all the species, and females can be diagnosed by the circular epigyne orifice and the longest copulatory ducts. To facilitate future identification, we also provide the GenBank accession codes of the DNA barcode gene, Cytochrome c oxidase subunit I (COI), for all the type specimens.

A new species of Denisiphantes Tu, Li & Rollard, 2005 (Araneae, Linyphiidae) from Yunnan, China.

Denisiphantes arcuatus sp. nov. (♂♀) is described from Yunnan, China. Detailed descriptions of somatic features, genitalic characters, photos of habitus of the new species as well as of copulatory organs of Denisiphantes denisi (Schenkel, 1963) (♂♀) are presented. A distribution map is also provided.

Characterization of cadmium-responsive transcription factors in wolf spider Pardosa pseudoannulata.

Transcription factors (TFs) act on the regulation of gene expression, which is prevalent in all organisms, and their characterization may provide important clues for understanding the regulatory mechanism of gene expression. In this research, inhibited growth (delayed developmental time and decreased body weight) and increased activities of antioxidant enzymes (peroxidase, superoxide dismutase, and catalase) were recorded in Pardosa pseudoannulata in response to cadmium burden. Expression profiles of TFs were analyzed based on the transcriptome profiling of P. pseudoannulata, and 1711 TFs genes were differentially expressed with 995 up-regulated and 716 down-regulated. Most of the differentially expressed TFs belonged to zf-C2H2, ZBTB, Homeobox, and bHLH families. Interestingly, hub genes smads, TCF7L2, EGR1, and GATA5 were identified to be the candidate Cd-responsive TFs related to growth of spider. The expression level of Sod2 (superoxide dismutase) was regulated by the up-regulated TF foxo3, implying its important role in the antioxidant defense of spider. Moreover, sequence analysis demonstrated that smads and foxo3 were conserved among spiders and insects. This study revealed for the first time the role of TFs in molecular response of P. pseudoannulata to Cd stress, providing the basis for the protection of tarantula under Cd stress.

Four new species of the spider genus Synagelides Strand, 1906 from South China (Araneae, Salticidae).

Four new species of the jumping spider genus Synagelides Strand, 1906 from Guizhou and Yunnan, China are described: Synagelidesangustus sp. nov. (♀), S.latus sp. nov. (♂♀), S.subagoriformis sp. nov. (♂♀), and S.triangulus sp. nov. (♀). Photographs of the habitus and copulatory organs and a distributional map are provided.

Dolichognatha bannaensis new species, a six-eyes tetragnathid spider from China (Araneae, Tetragnathidae).

Almost all of the tetragnathid spiders have eight eyes, while two Dolichognatha species, D. longiceps (Thorell, 1895) (Sunil Jose 2014: 474, figs 1A-B) and D. junlitjri (Barrion-Dupo Barrion, 2014) (Barrion-Dupo Barrion 2014: 196, figs 1A-B) have six eyes (PME absent). They were originally assigned to Prolochus Thorell, 1895, which was synonymized with Dolichognatha O. Pickard-Cambridge, 1869 by Levi (1981), and reaffirmed by Kallal Hormiga (2018) after a multi-loci phylogenetic analysis.

On two species of the spider genus Stertinius Simon, 1890 from China (Araneae: Salticidae).

Stertinius Simon, 1890 belongs in the subtribe Simaethina Simon, 1903 of the subfamily Salticinae Blackwall, 1841 (Maddison 2015), which represents a group of small spiders with flatted prosoma and conspicuous, sclerotized sigillae (Logunov et al. 1997; Logunov 2018). Currently, a total of 12 species have been described mainly from East and Southeast Asia, of which five species have only one sex described (World Spider Catalog 2020).

Mul-tiomics analysis of cadmium stress on the ovarian function of the wolf spider Pardosa pseudoannulata.

Cadmium (Cd) pollution is widespread in paddy filed soil in China. In this study, the toxicity of Cd with regard to the female reproductive system of paddy spider Pardosa pseudoannulata was investigated by means of multi-omics analyses (transcriptome, proteome, and miRNAs). Decreased activities of detoxifying enzymes including peroxidase (POD), Glutathione S-transferases (GST), and superoxide dismutase were detected in the ovary of P. pseudoannulata. Of these, GST and POD were consistently down-regulated at the transcriptional and translational levels. Vitellogenin content and fecundity of the spider were also reduced by Cd burden. Five vitellogenin encodes genes were down-regulated while only vitellogenin-6 protein was up-regulated. But protein lipovitellin-1, the main composition of vitellin, was down-regulated. In addition, the correlation between the mitogen-activated protein kinase (MAPK) signaling pathway and Cd stress was identified. A down-regulated gene that encoding connector of kinase to AP-1 in the MAPK signaling pathway was regulated by the up-regulated miRNA (miRNA id: miRNA dan-miR- 318>der-miR-318>dgr-miR-318>dme-miR-318-3p > dmo-miR-318>dpe-miR-318>dps-miR-318>dse-miR-318>dsi-miR-318>dvi-miR-318>dwi-miR-318>dya-miR-318). In conclusion, Cd stress possesses distinct female reproductive toxicity on P. pseudoannulata through impairing antioxidant system and synthesis of vitellin.

Six new species of Otacilia from southern China (Araneae: Phrurolithidae).

Six new ground sac spider species of the family Phrurolithidae were collected from southern China: Otacilia ailan sp. nov. (♀), O. daweishan sp. nov. (♂, ♀), O. fabiformis sp. nov. (♂, ♀) and O. jiandao sp. nov. (♀) from Hunan Province; and O. nonggang sp. nov. (♂, ♀) and O. yinae sp. nov. (♂) from Guangxi Zhuang Autonomous Region. All species are described and illustrated with photographs and line drawings.

Four new species of the spider genus Cicurina Menge, 1871 from China (Araneae: Dictynidae).

Four new species of the genus Cicurina are described: C. daweishanensis spec. nov. (female), C. kekei spec. nov. (male and female), C. lichuanensis spec. nov. (male and female) and C. yuelushanensis spec. nov. (male and female). Photos of body and copulatory organs, line drawings of copulatory organs, as well as the locality map are provided. The type specimens of Cicurina eburnata Wang, 1994 were reexamined for the comparison of allied species. Photos of C. calyciforma Wang Xu, 1989 are also presented in order to compare it with two new species.

Five new species of Otacilia Thorell, 1897 (Araneae: Phrurolithidae) from the Wuling Mountain Range, China.

Five new species of the genus Otacilia Thorell, 1897 from the Wuling Mountain Range in China are described here based on male and female specimens: O. acerosa sp. nov., O. lata sp. nov., O. palmata sp. nov., O. rulinensis sp. nov. and O. xingdoushanensis sp. nov.. Detailed morphological descriptions, photographs of the habitus and genital organs, line drawings, and locality maps are provided.

Redescription of Clubiona gongi Zhang, Yin, Bao amp; Kim, 1997 (Araneae: Clubionidae) with the first description of the male.

Clubiona Latreille, 1804 comprises 503 species across the world, of which 122 species were reported from China. Nearly one-third of Chinese species have been described with single-sex (World Spider Catalog, 2018). Twenty-eight species have been reported from Guizhou Province (Wang et al. 2015; Wu et al. 2015; Li Lin 2016; Yu et al. 2017; Wang et al. 2018; Zhang et al. 2018).