ABSTRACT
The complete mitochondrial genome of the Scarlet Tiger moth Callimorpha dominula (Insecta: Lepidoptera: Arctiidae) has been reconstructed from the whole-genome Illumina sequencing data. This circular genome is 15 496 bp in size, and contains 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one A + T-rich D-loop or control region. Most PCGs are initiated with the ATN codons, except for COX1 with the unusual CGA as its initiation codon. Four PCGs (COX1, COX2, ND3, and ND4) are terminated with incomplete codon T, ND4L uses TAG as its termination codon, while all the other eight PCGs employ the usual ATN codons. The nucleotide composition is highly asymmetric (40.1% A, 40.9% T, 7.6% G, and 11.4% C) with an overall A + T content of 81.0%. The phylogenetic analysis based on the neighbor-joining (NJ) method suggests that C. dominula is more phylogenetically related to its confamilial counterparts than to those from other families.
Subject(s)
Genome, Mitochondrial , Moths/genetics , Animals , DNA, Mitochondrial/genetics , Insect Proteins/genetics , Mitochondrial Proteins/genetics , Phylogeny , Whole Genome SequencingABSTRACT
The complete mitochondrial genome of the wild silk moth Bombyx huttoni has been reconstructed from the whole-genome Illumina sequencing data. The circular genome was determined to be 15,638 bp in length, consisting of 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one A+T-rich D-loop or control region. Twelve PCGs (COX2, COX3, CYTB, ND1, ND2, ND3, ND4, ND4L, ND5, ND6, ATP6, and ATP) are initiated with the typical ATN codons, whereas COX1 was tentatively determined to use the unusual CGA as its start codon. All PCGs are terminated with the TAA codon, except for ND1 and the other two PCGs (COX1 and COX2) with the TAG and the incomplete T stop codon, respectively. The nucleotide composition is highly asymmetric (43.8% A, 38.0% T, 7.1% G, and 11.1% C) with an overall A+T content of 81.8%.
Subject(s)
Bombyx/genetics , Butterflies/genetics , Genome, Mitochondrial/genetics , Lepidoptera/genetics , Mitochondria/genetics , Animals , Base Composition/genetics , Codon, Initiator/genetics , Codon, Terminator/genetics , Genome Size/genetics , RNA, Ribosomal/genetics , RNA, Transfer/genetics , Sequence Analysis, DNA/methodsABSTRACT
The complete mitochondrial genome of the Sara Longwing Heliconius sara has been reconstructed from the whole-genome Illumina sequencing data. The mitochondrial genome is 15,372 bp in size with the highly asymmetric overall A + T content of 80.6%. Annotation of mitochondrial genome revealed a total of 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs) and 1 D-loop region. Most PCGs are initiated with the ATN codons, while COX1 and ND1 start with CGA and TTG, respectively. COX1, COX2, and ND4 harbor the incomplete stop codon T, while all the others are terminated with the TAR codons. The complete mitochondrial genome of H. Sara would contribute to our further understanding of the phylogeny and evolution of the genus Heliconius and related taxa.
