ABSTRACT
Since temperature is one of the most important physical parameters, it is of great significance to measure temperature with high space resolution and accuracy. Herein, a series of π-conjugated triarylboron compounds with temperature sensitivity based on naphthalene aromatic groups were designed and synthesized. Their photophysical properties were studied in detail by steady state absorption, emission spectroscopy and time-dependent density functional theory calculation. As the temperature increased, their emission spectra exhibited obvious blue shifts in liquid organic solvent, making ratiometric fluorescence sensing of temperature achievable.
ABSTRACT
Currently, there are more than 200 fecal microbiota transplantation (FMT) clinical trials worldwide. However, our knowledge of this microbial therapy is still limited. Here we develop a strategy using sequential tagging with D-amino acid-based metabolic probes (STAMP) for assessing the viabilities of transplanted microbiotas. A fluorescent D-amino acid (FDAA) is first administered to donor mice to metabolically label the gut microbiotas in vivo. The labeled microbiotas are transplanted to recipient mice, which receive a second FDAA with a different color. The surviving transplants should incorporate both FDAAs and can be readily distinguished by presenting two colors simultaneously. Isolation of surviving bacteria and 16S rDNA sequencing identify several enriched genera, suggesting the importance of specific bacteria in FMT. In addition, using STAMP, we evaluate the effects on transplant survival of pre-treating recipients using different antibiotics. We propose STAMP as a versatile tool for deciphering the complex biology of FMT, and potentially improving its treatment efficacy.
