ABSTRACT
OBJECTIVE: To investigate the effect of X-ray radiation at the median lethal dose (LD50) on the outcome of a cluster of differentiation 133 (CD133)- cells in nasopharyngeal carcinoma. MATERIALS AND METHODS: CD133- cells were obtained from human nasopharyngeal carcinoma cells (CNE-1 and CNE-2) based on CD133-labeled fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS), respectively. Changes in invasion ability and in-vivo tumorigenicity of CD133- cells before and after X-ray radiation at LD50 were observed. Moreover, CD133, SRY-related HMG-box 2 (SOX2), and organic carnitine transporter 4 (OCT4) expression changes were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. RESULTS: The invasion ability and in-vivo tumorigenicity of CD133+ cell subsets were significantly stronger than those of CD133- cell subsets. After X-ray radiation at LD50, the invasion ability of CD133- cell subsets and in-vivo tumorigenicity were significantly increased. RT-PCR and Western blotting results manifested that the expression levels of CD133, SOX2, and OCT4 were remarkably up-regulated after radiation. CONCLUSIONS: X-ray radiation at LD50 can enhance the stemness potential by up-regulating the expression of stemness-related genes in nasopharyngeal carcinoma CD133- cells.
Subject(s)
AC133 Antigen/metabolism , Up-Regulation/radiation effects , X-Rays , Animals , Cell Line, Tumor , Cell Movement/drug effects , Cell Survival/drug effects , Humans , Lethal Dose 50 , Mice , Mice, Nude , Nasopharyngeal Carcinoma/metabolism , Nasopharyngeal Carcinoma/pathology , Nasopharyngeal Carcinoma/radiotherapy , Nasopharyngeal Neoplasms/metabolism , Nasopharyngeal Neoplasms/pathology , Nasopharyngeal Neoplasms/radiotherapy , Octamer Transcription Factor-3/metabolism , SOXB1 Transcription Factors/metabolism , Transplantation, HeterologousABSTRACT
It was found that Ginsenoside B (GSB) could reduce the myocardial systolic power and frequency of the isolated atrium of guinea pigs in a concentration-dependent way, and obviously prolong the functional refractory period of the left atrium. GSB could competitively antagonize the positive inotropic action of Iso, and non-competitively antagonize the positive inotropic action of CaCl2 on the isolated right atrium of guinea pigs.
Subject(s)
Calcium Channel Blockers/pharmacology , Ginsenosides , Heart Rate/drug effects , Myocardial Contraction/drug effects , Refractory Period, Electrophysiological/drug effects , Saponins/pharmacology , Animals , Calcium Chloride/antagonists & inhibitors , Depression, Chemical , Female , Guinea Pigs , Heart Atria , In Vitro Techniques , Isoproterenol/antagonists & inhibitors , MaleABSTRACT
The experimental results show that the Maren soft capsule can increase the amount and weight of the stool of normal mice and model mice with dry stool, promote the advanced percentage of charcoal powder in the small and large intestines of mice, enhance the movement of the smooth muscle of isolated ileum of guinea pigs under physiological conditions or low temperature, as well as strengthen the intestinal movement in rabbits.
