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1.
J Med Virol ; 91(12): 2125-2133, 2019 12.
Article in English | MEDLINE | ID: mdl-31429939

ABSTRACT

Immunosuppressive therapy causes severe impairment of host defense and diarrhea is a frequent complication in renal transplant recipients. This study aimed to describe the occurrence of Rotavirus A (RVA) and Human Bocavirus (HBoV) in fecal samples of immunosuppressed patients submitted to renal transplantation during posttransplant follow-up. A longitudinal study was carried out involving a 25-patient cohort, selected for kidney transplantation. A total of 126 fecal samples were collected between May 2014 and May 2016. Molecular techniques were used to detect and characterize circulating RVA and HBoV genotypes and statistical analysis were applied to verify the association between epidemiological and clinical characteristics. The prevalence of RVA and HBoV was 24% (6/25) and 40% (10/25), respectively. Among RVA and HBoV positive cases, the majority was female; did not conduct water treatment nor had adequate sewage facilities. The most detected genotypes were RVA G3 (62.5%) and HBoV-3 (95%). Phylogenetic analysis of HBoV strains indicated that studied samples were similar to those found in Asian and American countries. The present study point out the circulation of these viral agents among immunosuppressed individuals and these findings will enable the construction of new knowledge and care perspectives on the cause of diarrhea in this population.


Subject(s)
Feces/virology , Human bocavirus/isolation & purification , Immunocompromised Host , Kidney Transplantation/adverse effects , Rotavirus/isolation & purification , Adult , Brazil/epidemiology , Coinfection/epidemiology , Coinfection/virology , Female , Genotype , Human bocavirus/genetics , Humans , Longitudinal Studies , Male , Parvoviridae Infections/epidemiology , Parvoviridae Infections/virology , Phylogeny , Prevalence , Rotavirus/genetics , Rotavirus Infections/epidemiology , Rotavirus Infections/virology , Sequence Analysis, DNA , Transplant Recipients/statistics & numerical data
2.
J Med Virol ; 89(6): 974-981, 2017 06.
Article in English | MEDLINE | ID: mdl-27862014

ABSTRACT

The species A rotaviruses (RVA) are important gastroenteric pathogens that infect humans and animals. RVA genotype G3P[9] has been described in human-animal reassortment events, and the complexity of its hosts motivates the genetic investigation of this strain. Therefore, the aim of this study is to analyse a G3P[9] sample that was detected in a child with acute gastroenteritis. The 1A3739 sample featured the constellation G3P[9]-I18-R3-C3-Mx-A19-N3-T3-E3-H6. The sequence for VP3 gene was not obtained. The phylogeny showed a closer relationship among genes VP7, VP1, NSP3, NSP4, and NSP5 with genes of animal origin, such as chiropter, alpaca, equine, and simian. In addition, the genes VP6 and NSP1 belong to the new genotypes I18 and A19, respectively. The emergence of strains such as these can interfere with the effectiveness of the RVA vaccine, and continuous monitoring is therefore important. Additional studies are needed to determine the evolutionary source and to identify a possible reservoir of RVA in nature.


Subject(s)
Gastroenteritis/virology , Genotype , Reassortant Viruses/genetics , Rotavirus Infections/virology , Rotavirus/genetics , Child, Preschool , Evolution, Molecular , Female , Humans , Phylogeny , Recombination, Genetic , Rotavirus/isolation & purification , Sequence Analysis, DNA
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