ABSTRACT
OBJECTIVE: To determine the presence and concentration of CA-125 in periovulatory follicular fluid (FF) and serum after controlled ovarian hyperstimulation and to determine if the CA-125 in these two compartments could be related to granulosa cell markers such as inhibin or estradiol. STUDY DESIGN: Fifteen women undergoing controlled ovarian hyperstimulation for in-vitro fertilization-embryo transfer were studied. A transvaginal, ultrasound-guided follicular aspiration was performed. CA-125, inhibin, estradiol and FSH were measured in FF and serum. Pearson and Spearman's Rank Correlation tests were performed. RESULTS: CA-125 was measurable in 59% of follicles. Values ranged from undetectable to 3630 U/ml. Serum CA-125 ranged from undetectable to 126 U/ml. CA-125 and inhibin correlated negatively in FF and positively in serum. CONCLUSION: CA-125 was present in significant but variable concentrations in 59% of periovulatory follicles. A negative correlation was noted between CA-125 and inhibin or estradiol in the FF and a positive correlation with serum inhibin. No correlations were noted to oocyte retrieval or fertilization.
Subject(s)
CA-125 Antigen/metabolism , Follicular Fluid/metabolism , Granulosa Cells/metabolism , Hormones/metabolism , Ovulation Induction , Ovulation/physiology , Adult , CA-125 Antigen/blood , Embryo Transfer , Estradiol/blood , Estradiol/metabolism , Female , Fertilization in Vitro , Follicle Stimulating Hormone/blood , Follicle Stimulating Hormone/metabolism , Humans , Inhibins/blood , Inhibins/metabolismABSTRACT
An investigation of the hydrolysis of Tween 80 reagent by Branhamella catarrhalis and related organisms (Neisseria and Moraxella species) revealed that only B. catarrhalis gave a positive result. A total of 226 strains, including reference organisms and clinical isolates, were studied. B. catarrhalis changed the color of the reagent from amber to pink-red after overnight incubation. We recommend this simple and cost-effective test as an alternative procedure to DNase testing or tributyrin hydrolysis or as a supplemental procedure for the identification of B. catarrhalis in clinical specimens.
Subject(s)
Bacteriological Techniques , Moraxella catarrhalis/isolation & purification , Polysorbates , Evaluation Studies as Topic , Humans , Hydrolysis , Indicators and ReagentsABSTRACT
Routine autopsy studies of hearts with coronary thrombosis, collected over a period of eleven years, showed unique morphologic features of rupture of soft atheromatous plaques. These features include: (1) irregular luminar outline: angulation, invagination, upheaval, and/or wrinkles, (2) wavy outline of atheromatous cavity, (3) volcano-like rupture with seepage or gushing of semiliquid atheromatous contents into lumen, (4) cholesterol crystals arranged in rheologic vector direction, (5) rupture of deeper intimal fibrous tissue, (6) one or multiple intimal upheavals, (7) focal thickening of contracted media, (8) vestige of volcano-like eruption with organized thrombus, and (9) old thrombotic occlusion with wavy appearance of thick intimal fibrous tissue. The authors present a dynamic model of vasoconstriction or artery spasm that accounts for these features and the rupture of soft atheromatous plaques.
Subject(s)
Coronary Artery Disease/pathology , Coronary Vasospasm/pathology , Adult , Aged , Aged, 80 and over , Autopsy , Coronary Thrombosis/pathology , Coronary Vasospasm/complications , Female , Humans , Male , Middle Aged , Myocardial Infarction/etiology , Myocardial Infarction/pathology , Rupture, SpontaneousABSTRACT
Acute cytomegalovirus infection was associated with two episodes of morbilliform eruption in a patient receiving ampicillin therapy. Shortly after the eruption cleared, the patient died. Histologic examination of the skin indicated a cytomegalovirus infection of the vascular endothelium with luminal obliteration, stasis, vasculitis, and thrombus formation.
Subject(s)
Cytomegalovirus Infections/diagnosis , Skin Diseases, Infectious/diagnosis , Aged , Ampicillin/adverse effects , Cytomegalovirus Infections/complications , Erythema/etiology , Humans , Male , Skin/blood supply , Skin Diseases, Infectious/complications , Thrombosis/complications , Vasculitis/complicationsABSTRACT
Following massive resection of the small intestine, including the ileocecal valve in seven adult mongrel dogs, the remaining 20 centimeters of the proximal part of the jejunum was anastomised to the midtransverse portion of the colon. All dogs were given parenteral nutrition for four weeks. Six weeks after resection, the dogs underwent reexploration, and gross and microscopic examinations of the remaining part of the jejunum were carried out. Grossly, there was a 29.5 per cent increase in the length and a 31 per cent increase in the circumference of the intestine. Microscopically, there was a 23 per cent increase in the depth of the crypts, a 21 per cent increase in the number of villi and a 22 per cent increase in the width of the villi. No change was observed in the height of the villi.
