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1.
Neuroscience ; 147(1): 28-36, 2007 Jun 15.
Article in English | MEDLINE | ID: mdl-17499931

ABSTRACT

Alzheimer's disease is characterized by the presence of senile plaques in the brain, composed mainly of aggregated amyloid-beta peptide (Abeta), which plays a central role in the pathogenesis of Alzheimer's disease and is a potential target for therapeutic intervention. Amyloid plaques occur in an increasing number of brain structures during the progression of the disease, with a heavy load in regions of the temporal cortex in the early phases. Here, we investigated the cognitive deficits specifically associated with amyloid pathology in the entorhinal cortex. The amyloid peptide Abeta(1-42) was injected bilaterally into the entorhinal cortex of rats and behavioral performance was assessed between 10 and 17 days after injection. We found that parameters of motor behavior in an open-field as well as spatial working memory tested in an alternation task were normal. In contrast, compared with naive rats or control rats injected with saline, rats injected with Abeta(1-42) showed impaired recognition memory in an object recognition task and delayed acquisition in a spatial reference memory task in a water-maze, despite improved performance with training in this task and normal spatial memory in a probe test given 24 h after training. This profile of behavioral deficits after injection of Abeta(1-42) into the entorhinal cortex was similar to that observed in another group of rats injected with the excitotoxic drug, N-methyl-d-aspartate. Immunohistochemical analysis after behavioral testing revealed that Abeta(1-42) injection induced a reactive astroglial response and plaque-like deposits in the entorhinal cortex. These results show that experimentally-induced amyloid pathology in the entorhinal cortex induces selective cognitive deficits, resembling those observed in early phases of Alzheimer's disease. Therefore, injection of protofibrillar-fibrillar Abeta(1-42) into the entorhinal cortex constitutes a promising animal model for investigating selective aspects of Alzheimer's disease and for screening drug candidates designed against Abeta pathology.


Subject(s)
Alzheimer Disease/pathology , Amyloid beta-Peptides/metabolism , Disease Models, Animal , Entorhinal Cortex/pathology , Peptide Fragments/metabolism , Plaque, Amyloid/metabolism , Recognition, Psychology/physiology , Alzheimer Disease/complications , Alzheimer Disease/metabolism , Amyloid beta-Peptides/administration & dosage , Animals , Cognition Disorders/chemically induced , Cognition Disorders/complications , Cognition Disorders/metabolism , Cognition Disorders/pathology , Entorhinal Cortex/metabolism , Entorhinal Cortex/physiopathology , Exploratory Behavior/physiology , Gliosis/chemically induced , Gliosis/metabolism , Male , Maze Learning/physiology , Microinjections , N-Methylaspartate , Neurotoxins , Peptide Fragments/administration & dosage , Plaque, Amyloid/pathology , Rats , Rats, Wistar , Statistics, Nonparametric
2.
Neurobiol Dis ; 18(3): 499-508, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15755677

ABSTRACT

Short fragments and fragment analogues of beta-amyloid 1-42 peptide (Abeta1-42) display a protective effect against Abeta-mediated neurotoxicity. After consideration of our earlier results with in vitro bioassay of synthetic Abeta-recognition peptides and toxic fibrillar amyloids, five pentapeptides were selected as putative neuroprotective agents: Phe-Arg-His-Asp-Ser amide (Abeta4-8) and Gly-Arg-His-Asp-Ser amide (an analogue of Abeta4-8), Leu-Pro-Tyr-Phe-Asp amide (an analogue of Abeta17-21), Arg-Ile-Ile-Gly-Leu amide (an analogue of Abeta30-34), and Arg-Val-Val-Ile-Ala amide (an analogue of Abeta38-42). In vitro electrophysiological experiments on rat brain slices demonstrated that four of these peptides counteracted with the field excitatory postsynaptic potential-attenuating effect of Abeta1-42; only Arg-Val-Val-Ile-Ala amide proved inactive. In in vivo experiments using extracellular single-unit recordings combined with iontophoresis, all these pentapeptides except Arg-Val-Val-Ile-Ala amide protected neurons from the NMDA response-enhancing effect of Abeta1-42 in the hippocampal CA1 region. These results suggest that Abeta recognition sequences may serve as leads for the design of novel neuroprotective compounds.


Subject(s)
Amyloid beta-Peptides/physiology , Amyloid/physiology , Neurons/physiology , Neuroprotective Agents/pharmacology , Oligopeptides/physiology , Peptide Fragments/physiology , Action Potentials/drug effects , Action Potentials/physiology , Amyloid beta-Peptides/ultrastructure , Animals , Electrophysiology , Male , N-Methylaspartate/pharmacology , Neurons/drug effects , Neurons/ultrastructure , Neuroprotective Agents/isolation & purification , Neuroprotective Agents/metabolism , Oligopeptides/isolation & purification , Peptide Fragments/ultrastructure , Rats , Rats, Wistar
3.
Neuropharmacology ; 42(3): 421-7, 2002 Mar.
Article in English | MEDLINE | ID: mdl-11897120

ABSTRACT

Neonatal maternal deprivation permanently modifies the hypothalamo-pituitary-adrenal (HPA) axis and other neurobiological and behavioural parameters in rats. The HPA axis plays a central role in the control of feeding, and participates in the anorexigenic action of dexfenfluramine and restraint stress, and in the orexigenic action of a cafeteria diet. Therefore, we investigated whether maternal deprivation modifies feeding responses to these factors. Experimental pups were separated for 24h from the mother 5 or 14 days after birth. The anorexigenic response to both dexfenfluramine and restraint stress was increased, and body weight as well as subcutaneous adipose tissue gain induced by cafeteria diet was higher in early deprived adult rats. However, these effects were dependent on the time of maternal deprivation. According to our predictions, the feeding response of maternally deprived rats to anorexigenic and orexigenic agents was altered, which is probably partly due to an altered HPA function, but the participation of the serotonergic, the opioid and/or the dopaminergic system cannot be ruled out. Additional studies are needed to detail precisely the neurobiological substrates of modified feeding behaviour of maternally deprived animals. This early stress paradigm altering feeding behaviour could become an interesting model for research into human eating disorders.


Subject(s)
Animals, Newborn/physiology , Feeding Behavior/drug effects , Feeding Behavior/physiology , Maternal Deprivation , Age Factors , Animals , Animals, Newborn/psychology , Behavior, Animal/drug effects , Behavior, Animal/physiology , Dexfenfluramine/pharmacology , Eating/drug effects , Eating/physiology , Eating/psychology , Feeding Behavior/psychology , Male , Rats , Rats, Long-Evans , Restraint, Physical/methods , Serotonin Receptor Agonists/pharmacology , Stress, Psychological/physiopathology
4.
Eur J Neurosci ; 14(4): 747-55, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11556899

ABSTRACT

The hypothalamo-pituitary-adrenal (HPA) axis plays a central role both in the regulation of the stress response, and in the control of feeding behaviour. Sensitivity of the HPA axis to respond to stress varies both during ontogeny and between individuals, and can be altered by neonatal events. The aim of our experiments was to determine whether early events that affect the HPA axis could also induce persistent modifications in food intake (quantitatively and qualitatively), as well as alterations of anxiety-related behaviour. Twenty-four-hour maternal deprivation was introduced at two different periods of HPA maturation, on day 5 (DEP5) or day 14 (DEP14) after birth. Sequential measurements of plasma levels of adrenocorticotropin hormone (ACTH) and corticosterone showed that this deprivation altered the HPA axis of adults; the response to restraint stress was prolonged in DEP5 and a higher ACTH peak appeared in DEP14. The neonatal stress also produced long-lasting modifications of rat behaviour, as DEP14 adults became more anxious. Standard food intake decreased in both groups of deprived rats. Diet preferences also changed, as carbohydrate intake decreased in DEP5 rats. Corticosteroid receptor binding did not vary in the hippocampus of the deprived rats. The modifications of the stress response and the behaviour parameters could be due to the alteration of corticosteroid receptors in the hypothalamic paraventricular nucleus and/or corticotropin-releasing hormone or vasopressin function, but these parameters have yet to be determined. This early stress paradigm altering feeding behaviour could become an interesting model for research into human eating disorders.


Subject(s)
Behavior, Animal/physiology , Feeding Behavior/physiology , Hypothalamo-Hypophyseal System/growth & development , Maternal Deprivation , Pituitary-Adrenal System/growth & development , Rats, Long-Evans/physiology , Stress, Physiological/physiopathology , Adrenocorticotropic Hormone/blood , Animals , Animals, Newborn , Body Weight/physiology , Corticosterone/blood , Disease Models, Animal , Eating/physiology , Female , Hippocampus/metabolism , Hypothalamo-Hypophyseal System/metabolism , Male , Motor Activity/physiology , Nutritional Physiological Phenomena/physiology , Pituitary-Adrenal System/metabolism , Rats , Receptors, Glucocorticoid/drug effects , Receptors, Glucocorticoid/metabolism , Receptors, Mineralocorticoid/drug effects , Receptors, Mineralocorticoid/metabolism , Receptors, Steroid/metabolism , Restraint, Physical/adverse effects
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