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1.

Purification of polyribosomes, 70S, monomers or ribosomal subunits by gel filtration.

García-Patrone, M; Perazzolo, C A; Algranati, I D.

Acta Physiol Lat Am ; 26(5): 330-6, 1976.

Article in English | MEDLINE | ID: mdl-802625

ABSTRACT

Chromatography through Bio-Gel columns is a simple and rapid method for the purification of different ribosomal particles. This mild procedure, easily adapted to many purposes with good yields, can substitute the conventional sucrose gradient centrifugations, especially when the integrity of labile particles or complexes must be preserved.

Subject(s)

Polyribosomes/analysis , Cell Fractionation , Chromatography, Gel/methods , Escherichia coli/ultrastructure

2.

Purification of polyribosomes, 70S, monomers or ribosomal subunits by gel filtration.

García-Patrone, M; Perazzolo, C A; Algranati, I D.

Acta Physiol Lat Am ; 26(5): 330-6, 1976.

Article in English | BINACIS | ID: bin-48008

ABSTRACT

Chromatography through Bio-Gel columns is a simple and rapid method for the purification of different ribosomal particles. This mild procedure, easily adapted to many purposes with good yields, can substitute the conventional sucrose gradient centrifugations, especially when the integrity of labile particles or complexes must be preserved.

3.

Purification of polyribosomes, 70S, monomers or ribosomal subunits by gel filtration

García-Patrone, M; Perazzolo, C A; Algranati, I D.

Acta physiol. latinoam ; 26(5): 330-6, 1976.

Article in Spanish | LILACS-Express | BINACIS | ID: biblio-1158495

ABSTRACT

Chromatography through Bio-Gel columns is a simple and rapid method for the purification of different ribosomal particles. This mild procedure, easily adapted to many purposes with good yields, can substitute the conventional sucrose gradient centrifugations, especially when the integrity of labile particles or complexes must be preserved.

4.

Termination of polypeptide synthesis in bacteria and the exchange of ribosomal subunits.

Perazzolo, C A; Azzam, M E; Algranati, I D.

Eur J Biochem ; 34(3): 467-73, 1973 May 02.

Article in English | MEDLINE | ID: mdl-4577196

Subject(s)

Escherichia coli/metabolism , Peptide Chain Termination, Translational , Ribosomes/metabolism , Bacterial Proteins/biosynthesis , Carbon Isotopes , Cell Fractionation , Cell-Free System , Macromolecular Substances , Peptide Chain Elongation, Translational , Phosphorus Isotopes , Polyribosomes/analysis , RNA, Ribosomal/analysis , Spermidine

5.

The ribosome cycle in bacteria.

Algranati, I D; González, N S; García-Patrone, M; Perazzolo, C A; Azzam, M E.

Basic Life Sci ; 1: 327-38, 1973.

Article in English | MEDLINE | ID: mdl-4589685

Subject(s)

Bacterial Proteins/biosynthesis , Ribosomes/metabolism , Anti-Bacterial Agents/pharmacology , Cell Division , Centrifugation, Density Gradient , Escherichia coli/cytology , Escherichia coli/metabolism , Kinetics , Magnesium , Methionine , Models, Biological , Peptide Chain Termination, Translational , Peptides , Phosphorus Radioisotopes , Polyribosomes/metabolism , RNA, Bacterial/metabolism , RNA, Messenger/metabolism , RNA, Transfer/metabolism , Ribonucleosides/pharmacology , Ribosomes/drug effects , Species Specificity , Spectrophotometry, Ultraviolet , Spermidine/pharmacology , Time Factors , Ultracentrifugation

6.

Polypeptide synthesis in Escherichia coli extracts: Effect of spermidine on the exchange of ribosomal subunits.

Azzam, M E.; Perazzolo, C A.; Algranati, I D..

FEBS Lett ; 21(2): 165-168, 1972 Mar 15.

Article in English | MEDLINE | ID: mdl-11946501

7.

Studies on dissociation factor of bacterial ribosomes: effect of antibiotics.

García-Patrone, M; Perazzolo, C A; Baralle, F; González, N S; Algranati, I D.

Biochim Biophys Acta ; 246(2): 291-9, 1971 Aug 26.

Article in English | MEDLINE | ID: mdl-4943719

Subject(s)

Anti-Bacterial Agents/pharmacology , Bacillus/drug effects , Escherichia coli/drug effects , Ribosomes/drug effects , Antibiotics, Antineoplastic/pharmacology , Bacillus/cytology , Bacterial Proteins/pharmacology , Centrifugation, Density Gradient , Chloramphenicol/pharmacology , Chlortetracycline/pharmacology , Depression, Chemical , Drug Antagonism , Erythromycin/pharmacology , Escherichia coli/cytology , Fusidic Acid/pharmacology , Guanosine Triphosphate/antagonists & inhibitors , Guanosine Triphosphate/pharmacology , Kanamycin/pharmacology , Lincomycin/pharmacology , Macromolecular Substances , Neomycin/pharmacology , Puromycin/pharmacology , Stimulation, Chemical , Streptomycin/pharmacology , Tetracycline/pharmacology

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