Knee synergism during gait remain altered one year after acl reconstruction

OBJECTIVE: To compare the activation of the vastus lateralis (VL) and biceps femoris (BF) muscles during gait, as well VL/BF muscular co-contraction (MCC) between healthy (CG) and anterior cruciate ligament reconstructed (ACL-R) subjects. METHODS: Nineteen subjects, ten controls and nine ACL-R patients had a VL and BF electromyogram (EMG) captured to calculate the MCC ratio. A Principal Component (PC) Analysis was applied to reduce the dimensionality effect of each of the MCC, VL and BF curves for both healthy and ACL reconstructed groups. The PC scores were used to calculate the standard distance (SD). SD values were employed in order to compare each dependent variable (MCC, VL and BF) between the two groups using unpaired t-test. RESULTS: ACL-R group presented a lower VL activation at the beginning and at the end of the gait cycle, as compared to the control group. However, no difference was found for BF or VL/BF MCC. CONCLUSION: The gait analysis of ACL reconstructed patients demonstrated a persistent deficit in VL activation when compared to the control group, even one year after surgery. Level of Evidence III. Case Control Study

KNEE SYNERGISM DURING GAIT REMAIN ALTERED ONE YEAR AFTER ACL RECONSTRUCTION.

OBJECTIVE: To compare the activation of the vastus lateralis (VL) and biceps femoris (BF) muscles during gait, as well VL/BF muscular co-contraction (MCC) between healthy (CG) and anterior cruciate ligament reconstructed (ACL-R) subjects. METHODS: Nineteen subjects, ten controls and nine ACL-R patients had a VL and BF electromyogram (EMG) captured to calculate the MCC ratio. A Principal Component (PC) Analysis was applied to reduce the dimensionality effect of each of the MCC, VL and BF curves for both healthy and ACL reconstructed groups. The PC scores were used to calculate the standard distance (SD). SD values were employed in order to compare each dependent variable (MCC, VL and BF) between the two groups using unpaired t-test. RESULTS: ACL-R group presented a lower VL activation at the beginning and at the end of the gait cycle, as compared to the control group. However, no difference was found for BF or VL/BF MCC. CONCLUSION: The gait analysis of ACL reconstructed patients demonstrated a persistent deficit in VL activation when compared to the control group, even one year after surgery. Level of Evidence III. Case Control Study.

Uso de medidas espaço-temporais da marcha para determinar o retorno ao esporte pós-reconstrução do LCA / Use of spatiotemporal gait parameters to determine return to sports after ACL reconstruction

Objetivo: Comparar os parâmetros espaço temporais da marcha de sujeitos hígidos e pacientes submetidos à reconstrução do LCA, classificando o status de normalidade. Método: Quatorze sujeitos hígidos e oito com reconstrução do LCA há aproximadamente um ano caminharam enquanto o movimento era capturado por um sistema de câmeras infravermelhas. Os instantes de contato inicial e retirada do pé do solo foram determinados e as seguintes variáveis dependentes, as quais foram comparadas entre os grupos por meio do teste Mann-Whitney(α=0,05), foram calculadas: percentual de tempo no apoio duplo inicial, percentual de tempo no apoio simples, percentual de tempo no apoio duplo terminal, comprimento da passada e velocidade da marcha. Inicialmente, foi aplicada uma regressão logística a todas as variáveis dependentes para determinar os sujeitos hígidos e aqueles com reconstrução do LCA. Resultados: Os dois grupos não apresentaram diferenças em nenhum parâmetro espaço temporal da marcha(p > 0,05), apesar da cinemática angular do joelho permanecer alterada,como evidenciado por um estudo anterior com a amostra similar.Conclusão: A regressão classificou todos os sujeitos como hígidos,inclusive aqueles do grupo com reconstrução do LCA, sugerindo que as variáveis espaço temporais aplicadas nesse estudo não devem ser usadas como critério isolado de retorno incondicional às atividades esportivas. Nível de Evidência III, Estudo de Caso Controle.

Objective: To compare gait spatiotemporal parameters of healthyand ACL reconstructed subjects in order to classify the status of gaitnormality. Methods: Fourteen healthy subjects and eight patientssubmitted to ACL reconstruction walked along a walkway while thelower limbs movement was captured by an infrared camera system.The frames where the initial contact and toe-off took place were determinedand the following dependent variables, which were comparedbetween groups through the Mann-Whitney test (α=0.05) were calculated:percentage of time in initial double stance, percentage of timein single stance, percentage of time in terminal double stance, stridelength and gait velocity. Initially, all variables were compared betweengroups using a Mann-Whitney test. A logistic regression was applied,including all dependent variables, to create a model that could differentiatehealthy and ACL reconstructed subjects. Results: ACLreconstructed group showed no differences in any spatiotemporalparameter of gait (p > 0.05) in relation to the control group, althoughthe angular kinematic differences of the knee remained altered, asevidenced in a study with a similar sample. Conclusion: The regressionclassified all subjects as healthy, including the ACL reconstructedgroup, suggesting the spatiotemporal variables should not be usedas the sole criterion of return to sports activities at the same level asprior to injury. Level of Evidence III, Case Control Study.

USE OF SPATIOTEMPORAL GAIT PARAMETERS TO DETERMINE RETURN TO SPORTS AFTER ACL RECONSTRUCTION.

OBJECTIVE: : To compare gait spatiotemporal parameters of healthy and ACL reconstructed subjects in order to classify the status of gait normality. METHODS: : Fourteen healthy subjects and eight patients submitted to ACL reconstruction walked along a walkway while the lower limbs movement was captured by an infrared camera system. The frames where the initial contact and toe-off took place were determined and the following dependent variables, which were compared between groups through the Mann-Whitney test (a=0.05) were calculated: percentage of time in initial double stance, percentage of time in single stance, percentage of time in terminal double stance, stride length and gait velocity. Initially, all variables were compared between groups using a Mann-Whitney test. A logistic regression was applied, including all dependent variables, to create a model that could differentiate healthy and ACL reconstructed subjects. RESULTS: : ACL reconstructed group showed no differences in any spatiotemporal parameter of gait (p > 0.05) in relation to the control group, although the angular kinematic differences of the knee remained altered, as evidenced in a study with a similar sample. CONCLUSION: : The regression classified all subjects as healthy, including the ACL reconstructed group, suggesting the spatiotemporal variables should not be used as the sole criterion of return to sports activities at the same level as prior to injury. Level of Evidence III, Case Control Study.

Teste de força de preensão manual: estudo da fadiga mioelétrica do flexor radial do carpo e flexor superficial dos dedos / Handgrip strength test: a study of myoelectric fatigue of flexor carpi radialis and the flexor digitorum superficialis

Um protocolo de força de preensão manual (FPM) em degraus de intensidade foi empregado para estudo da fadiga dos músculos flexor radial do carpo (FRC) e flexor superficial dos dedos (FSD) por meio do registro da força de preensão sustentada. Foi feita a análise do sinal eletromiográfico de superfície destes músculos no domínio do tempo e da frequência de 2kHz. Foi utilizado um dinamômetro eletrônico e um conversor analógico-digital de 16 bits. Participaram deste estudo 12 indivíduos saudáveis, ativos e destros, com média de idade 21,53 ± 1,26 anos, percentual de gordura 7,76 ± 3,53%, peso corporal 74,9 ± 10,36 kg e estatura 180,69 ± 7,14 cm. Os indivíduos realizaram o teste de contração isométrica voluntária máxima (CIVM) da mão dominante seguido do protocolo de degraus submáximos em 20%, 40% e 60% da CIVM por 10 segundos cada. O processamento dos sinais envolveu a filtragem passa banda e o cálculo dos valores de raiz média quadrática (RMS) e frequência mediana (FM) em cada degrau de contração submáxima. A análise de variância "two-way" foi aplicada para os valores de RMS e FM. O teste proposto não gerou queda do rendimento de força nos degraus submáximos estabelecidos e a instauração do processo de fadiga do FSD. Por outro lado, o FRC apresentou sinais de fadiga mioelétrica sugerindo o processo de falência da FPM. Estes dados sugerem que a fadiga mioelétrica dos flexores dos dedos durante a FPM é um processo tardio à fadiga dos estabilizadores do punho. O protocolo em degrau de 60% parece desencadear o processo de fadiga mioelétrica do músculo FRC, mas não do FSD, baseado na análise da ativação muscular nos domínios do tempo e frequência...

A test protocol of handgrip strength in steps was used in order to study the fatigue of the flexor carpi radialis (FRC) and the flexor digitorum superficialis (FSD) muscles with signal processing in the time and frequency domains. It was used an electronic hand grip dynamometer, and a 16-bits analog-digital converter. The sampling frequency was 2 kHz. The study included 12 healthy subjects, active and right-handed, mean age 21.53 ± 1.26 years, body fat percentage 7.76 ± 3.53%, weight 74.9 ± 10.36 kg and height 180. 69 ± 7.14 cm. The subjects performed the test of maximal voluntary contraction (CIVM) of the dominant hand and then, submaximal protocol of ten seconds steps of 20%, 40% and 60% of CIVM. The proposed test didn't generate a drop on strength performance on the established submaximal steps, as well as the fatigue process establishment of FSD. On the other hand, the FRC showed myoelectric fatigue signs, suggesting stabilizing wrist collapse. These data suggest that myoelectric fatigue of the finger flexors during handgrip is a late process compared to wrist stabilizing fatigue. The protocol in 60% step seems to trigger the myoelectric fatigue muscle process of the FRC but not the FSD, based on analysis of muscle activation in time and frequency domains...

Reducing cross terms effects in the Choi-Williams transform of mioelectric signals.

This study aims at investigating the effect of removing the negative values of Choi-Wiliams distribution (CWD) related to the electromyogram (EMG) for visualization and instantaneous median frequency (IMF) estimation. Beyond the EMG signals from triceps surae and biceps brachialis, the CWD was applied in a simulated sinusoidal signal as like in stationary and non-stationary simulated EMG signals (SES). The CWD negative values of all simulated and EMG signals were removed. The IMF values were obtained for SES and EMG. The CWD IMF values from SES and EMG were thus compared with the IMF values from short time Fourier transform (STFT) by means of correlation. The suppression of negative values from the CWD reduced cross terms influence and improved visualization, as shown by the increased correlation coefficient between the IMF values. Before this suppression, the extracted IMF values showed large oscillation along the time, with various spurious values beyond 500Hz, which disappeared after the suppression. Moreover, this procedure seems to be especially useful for non-stationary signals.

Influence of a preventive training program on lower limb kinematics and vertical jump height of male volleyball athletes.

OBJECTIVE: To examine the influence of a preventative training program (PTP) on sagittal plane kinematics during different landing tasks and vertical jump height (VJH) in males. DESIGN: Six weeks prospective exercise intervention. PARTICIPANTS: Fifteen male volleyball athletes (13 ± 0.7 years, 1.70 ± 0.12 m, 60 ± 12 kg). INTERVENTIONS: PTP consisting of plyometric, balance and core stability exercises three times per week for six weeks. Bilateral vertical jumps with double leg (DL) and single leg (SL) landings were performed to measure the effects of training. MAIN OUTCOME MEASUREMENTS: Kinematics of the knee and hip before and after training and VJH attained during both tasks after training. The hypothesis was that the PTP would produce improvements in VJH, but would not generate great changes in biomechanical behavior. RESULTS: The only change identified for the SL was the longest duration of landing, which represents the time spent from initial ground contact to maximum knee flexion, after training, while increased angular displacement of the knee was observed during DL. The training did not significantly alter the VJH in either the SL (difference: 2.7 cm) or the DL conditions (difference: 3.5 cm). CONCLUSIONS: Despite the PTP's effectiveness in inducing some changes in kinematics, the changes were specific for each task, which highlights the importance of the specificity and individuality in selecting prevention injury exercises. Despite the absence of significant increases in the VJH, the absolute differences after training showed increases corroborating with the findings of statistically powerful studies that compared the results with control groups. The results suggest that short-term PTPs in low risk young male volleyball athletes may enhance performance and induce changes in some kinematic parameters.

Comparação da ativação mioelétrica do glúteo máximo e bíceps femoral entre os agachamentos paralelo e com passada à frente / Comparison of myoelectric activity of gluteus maximus and biceps femoris between parallel and lunge squat / Comparación de la activación mioeléctrica de los glúteos y bíceps femoral entre las sentadillas con los pies paralelos y los pies uno frente al otro

O objetivo desse estudo foi comparar a atividade mioelétrica (EMG) do glúteo máximo e bíceps femoral entre o agachamento com passada à frente e com pés paralelos. Sete indivíduos realizaram oito repetições do agachamento paralelo (AP) e com passada à frente (AF) deslocando sobrecarga relativa a 50% da massa corporal. O EMG do glúteo máximo e bíceps femoral foi capturado, filtrado por um filtro passa-banda Butterworth de 4ª ordem (20-400 Hz) e obtido valores RMS. Utilizou-se o teste de Wilcoxon para comparar o EMG normalizado entre os dois exercícios. Tanto o bíceps femoral (p = 0,041) quanto o glúteo máximo (p = 0,0059) apresentaram maior ativação no AF (30,9% para glúteo; 42,6% para bíceps) em comparação ao AP (21,2% para glúteo; 24,7% para bíceps). Apesar da ativação moderada durante a execução dos exercícios, girando em torno de 25% a 45%, a resposta foi maior, para os participantes do estudo, no AF.

The purpose of this study was to compare the EMG of the gluteus maximus and biceps femoris between the lunge and the parallel squat. Seven subjects, with experience in strength training, performed eight repetitions of the parallel squat (PS) and the lunge (LU) with an overload corresponding to 50% of body mass. The EMG of the gluteus maximus and biceps femoris was captured, filtered by a forth order Butterworth filter (20-400 Hz) and calculated RMS values. The Wilcoxon Ranked test was used to compare the normalized EMG of each muscle between the two exercises. Both the biceps femoris (p = 0.041) and the gluteus maximus (p = 0.0059) showed increased activation in LU compared to the PS. Despite the moderate activation in both exercises, ranging from 25% to 45%, the myoelectric response of the analyzed muscles was higher, for the participants, in the lunge exercise.

El objetivo de este estudio fue comparar la actividad mioeléctrica (EMG) del músculo glúteo mayor y el bíceps femoral entre la sentadilla con los pies uno frente al otro y los pies paralelos. Siete sujetos realizaron ocho repeticiones de la sentadilla paralela (AP) y con los pies uno frente al otro (FA) desplazando una masa igual a 50% del peso corporal. El EMG del músculo glúteo mayor y el bíceps femoral fueron capturados, filtrada por un filtro de banda de 4 º orden Butterworth (20-400 Hz) y los valores RMS obtenidos. Se utilizó el test de Wilcoxon para comparar la EMG normalizada entre los dos ejercicios. Tanto el bíceps femoral (p = 0,041) y el glúteo mayor (p = 0,0059) mostraron una mayor activación en la FA (30,9% para lo glúteo y 52% para lo bíceps) en comparación con el AP (21,2% para lo glúteo y 24,7% para lo bíceps). Aunque la activación moderada durante la ejecución de los ejercicios fue moderado, situándose en torno a un 25% a 45%, la respuesta fue mayor en el FA para los participantes del estudio.

Ativação muscular do quadril e do joelho em duas aterrissagens realizadas por atletas do sexo masculino / Activation of hip and knee muscles during two landing tasks performed by male volleyball athletes

OBJETIVO: Comparar a atividade muscular antes e após o contato com o solo entre as aterrissagens unilateral (AU) e bilateral (AB) em atletas do sexo masculino. PARTICIPANTES: Quinze atletas masculinos de voleibol sem sinais e sintomas de lesões nas extremidades inferiores (13 ± 1 ano, 1,70 ± 0,12m, 60 ± 12kg). MENSURAÇÕES:Os participantes realizaram dois saltos verticais, aterrissando unilateralmente e bilateralmente. A atividade mioelétrica do reto femoral (RF), bíceps femoral (BF), adutores de quadril (AQ) e a relação BF/RF foram comparados entre as duas aterrissagens e entre as fases caracterizadas por 100ms antes (PRE) e 100ms após (POS) o contato com o solo. RESULTADOS: Em ambas as aterrissagens, a ativação do RF foi maior na fase POS em relação à PRE. Na comparação entre as aterrissagens dentro da mesma fase não encontramos diferenças estatísticas. Apesar de o BF não ter apresentado diferenças entre as fases PRE e POS em cada aterrissagem, sua ativação foi maior na AU. Os AQ apresentaram maior ativação na fase POS durante a AU, no entanto não houve diferenças quando comparadas as duas aterrissagens. A relação BF/RF apresentou valores maiores em ambas as aterrissagens na fase PRE. No entanto, não encontramos diferenças entre as aterrissagens. CONCLUSÃO: Os resultados sugerem que cada músculo apresenta um papel diferente durante a fase de aterrissagem em homens. Enquanto que o RF possui como principal função a frenagem da articulação do joelho e do movimento descendente, caracterizada pelo aumento da ativação na fase pós-contato, o BF parece atenuar a tensão articular do joelho em atividades de maior impacto, mantendo-se mais ativo durante todo o ciclo da AU. Já a maior ativação dos AQ após o contato com solo na AU evidencia a importância da região lombo-pélvica na estabilização pélvica em situações de grande instabilidade. Estudos futuros são necessários para determinar os efeitos da ativação muscular apresentada na imposição de cargas mecânicas potencialmente lesivas no joelho em atletas do sexo masculino.

OBJECTIVE: To compare the myoelectric activity before and after ground contact between single leg (SL) and double leg (DL) landings in male athletes. PARTICIPANTS: Fifteen male volleyball athletes without signs and symptoms of lesions in the lower extremities, with a minimum of three years experience in the sport (13 ± 1 years, 1.70 ± 0.12 m, 60 ± 12 kg). MEASUREMENTS: Participants performed two vertical jumps, landing unilaterally and bilaterally. The myoelectric activity of the rectus femoris (RF), biceps femoris (BF), hip adductors (HA) and the BF/RF ratio were compared between the two landings and between the phases characterized by 100ms before (PRE) and after 100 ms (POST) ground contact using ANOVA two-way test with post hoc test of Bonferroni (á = 5 percent). RESULTS: In both landings activation of RF was higher in the POST in relation to the PRE (p <0.0001). Comparing the landings in the same phase statistical differences (p = 0.2212) were not found. Although the BF did not present significant differences between the PRE and POST in each landing (p = 0.2321), its activation was higher in SL (p = 0.0051). The HA showed greater activation in the POST during the SL (p = 0.0013), however there were no differences when comparing the two landings (p = 0.9233). The BF/RF ratio was higher in both landings during PRE (p = 0.0012). Nevertheless, no differences between the landings (p = 0.7037) were found. CONCLUSION: The results suggest that each muscle has a different role during landing tasks in men. While RF has the main function to decelerate the knee and the downward movement, characterized by increased activation in the POST, BF seems to attenuate the loads on the knee in activities of higher impact, staying more active throughout the cycle in the SL. The increased activation of HA after ground contact in the SL highlights the importance of core region in stabilizing the pelvis in situations of great instability. Further studies are needed to determine the effects of muscle activation at the imposition of mechanical load on the knee that are potentially harmful to male athletes.

Isometric fatigue patterns in time and time-frequency domains of triceps surae muscle in different knee positions.

The occurrence of fatigue in triceps surae (TS) muscles during sustained plantar flexion contraction is investigated by means of the RMS electromyogram (EMG) and the instantaneous median frequency (IMF) of the short time Fourier transform (STFT). Six male subjects realized a 40% maximal plantar flexion isometric voluntary contraction until fatigue in two knee positions. Electrodes were positioned on gastrocnemius medialis, gastrocnemius lateralis and soleus muscles. The torque (TO) and EMG signals were synchronized. The RMS and the median of the IMF values were obtained, respectively, for each 250 ms and 1s windows of signal. Each signal was segmented into 10 epochs, from which the mean values of IMF, RMS and TO were obtained and submitted to linear regressions to determine parameter trends. Friedman test with the Dunn's post hoc were used to test for differences among muscles activation for each knee position and among slopes of regression curves, as well as to observe changes in TS RMS values over time. The results indicate different activation strategies with the knee extended (KE) in contrast to knee flexed (KF). With the KE, the gastrocnemii showed typical fatigue behavior with significant (p<0.05) IMF reductions and RMS increases over time, while soleus showed concomitant RMS and IMF increases (p<0.05) suggesting an increased soleus contribution to the torque production. With KF, the gastrocnemii were under activated, increasing the role of soleus. Thus, time-frequency analysis represented an important tool for TS muscular fatigue evaluation, allowing differentiates the role of soleus muscle.

Differences in time-frequency representation of lower limbs myoelectric activity during single and double leg landing in male athletes.

This study compared the instantaneous median frequency (IMF) obtained by means of a Choi-Williams transform of an electromyogram of the lower-limb muscles during single-leg (SL) and double-leg (DL) landings performed by fifteen male athletes. The IMF values of the rectus femoris (RF), biceps femoris (BF) and hip adductors (HA) were compared between two landing tasks, within each landing, and before and after ground contact (GC). The IMF values of the RF did not change between landings in contrast to those of the BF, which presented from 20- to 40-ms higher SL values before GC and from 40 to 60 ms after GC. HA presented higher SL values during the 40-60 ms range before GC. Within each landing, the RF IMF decreased from 40 ms to 60 ms after GC in the SL. Similar results were found for the HA IMF, which decreased from 40ms to 80 ms after GC. The BF IMF showed no significant change. These results suggest muscle recruitment related to anterior cruciate ligament protection since the IMF values of the RF decreased in the SL, whereas the BF IMF increased. Results for the HA showed the importance of hip muscles in stabilizing the core region, allowing the activation of distal muscles with greater safety.

Diferenças na cinemática entre dois tipos de aterrissagens em atletas de voleibol masculinos / Difference in kinematical behavior between two landing tasks in male volleyball athletes

As lesões no ligamento cruzado anterior (LCA) são frequentes no âmbito esportivo. O estudo dos mecanismos de lesão demonstrou que a maioria das lesões é oriunda tanto de aterrissagens unilaterais quanto bilaterais. Apesar de estudos terem demonstrado diferenças no comportamento cinemático de membros inferiores entre homens e mulheres, não há evidências de como homens se comportam em diferentes condutas. O objetivo neste estudo foi comparar a cinemática angular e temporal dos membros inferiores, manifestaem aterrissagens de saltos com propulsão bilateral, realizadas sobre um e dois membros inferiores. Aterrissagens unilaterais e bilaterais no plano frontal e sagital de quinze atletas masculinos de voleibol foram registradas e processadas por meio de técnicas videogramétricas. Os resultados indicam que, na aterrissagem unilateral, os atletas apresentaram menor flexão do quadril e joelho, assim como maior valgismo do joelho, quando comparada coma aterrissagem bilateral. A diferença no tempo de aterrissagem entre as duas condutas não foi estatisticamente significante. Em conclusão, os resultados deste estudo apóiam a premissa de que a cinemática dos membros inferiores se altera em função da configuração da conduta motora realizada em homens. Estudos futuros são necessários para explorar o impacto das diferenças cinemáticas encontradas na tensão ligamentar e relacioná-las comos mecanismos de lesões no LCA em homens.

Anterior cruciate ligament (ACL) injuries are common in sports. Studies investigating injury mechanisms have demonstrated that most injuries arise from landing tasks. Despite the demonstration of differences between male and female kinematics, there are no studies showing how males behave during different landing tasks. The objective of this study was to compare theangular and temporal kinematics of the lower limbs between two different landing tasks. Double leg and single leg landings were recorded in the frontal and sagittal plane in 15 male volleyball athletes by videogrammetry. Reduced hip and knee flexion and increased knee valgus were observed in the single leg landing task compared to the double leg landing task. No significantdifference in landing time was observed between the two tasks. In conclusion, the results support the premise that lower limb kinematics change according to the task performed. Further studies are necessary to explore the impact of these kinematic differences on knee loading and to relatethem to ACL injury mechanisms in men.

Influence of hip external rotation on hip adductor and rectus femoris myoelectric activity during a dynamic parallel squat.

This study sought to compare the myoelectric activity of the hip adductors (HAs) and rectus femoris (RF) when the hip was in a neutral position or externally rotated by 30° or 50° (H0, H30, and H50, respectively) during a parallel squat. Ten healthy subjects performed 10 repetitions of squats in each of the 3 hip positions and the myoelectric activities of the HAs and RF were recorded. The signal was then divided into categories representing concentric (C) and eccentric (E) contractions in the following ranges of motion: 0-30° (C1 and E1), 30-60° (C2 and E2), and 60-90° (C3 and E3) of knee flexion. From those signals, an root mean square (RMS) value for each range of motion in each hip position was obtained. All values were normalized to those obtained during maximum voluntary isometric contraction. We found that HAs showed a significant increase in myoelectric activity during C3 and E3 in the H30 and H50 positions, as compared with H0. Meanwhile, RF activity did not significantly differ between hip positions. Both muscles showed higher activation during 60-90° (C3 and E3) of knee flexion, as compared with 0-30° (C1 and E1) and 30-60° (C2 and E2). The results suggest that if the aim is to increase HA activity despite the low percentage of muscle activation, squats should be performed with 30° of external rotation and at least 90° of knee flexion.