ABSTRACT
Guided bone regeneration was studied to establish protocols and develop new biomaterials that revealed satisfactory results. The present study aimed to comparatively evaluate the efficiency of the bacterial cellulose membrane (Nanoskin®) and collagen membrane Bio-Gide® in the bone repair of 8-mm critical size defects in rat calvaria. Seventy-two adult male rats were divided into three experimental groups (n = 24): the CG-membrane-free control group (only blood clot, negative control), BG-porcine collagen membrane group (Bio-Guide®, positive control), and BC-bacterial cellulose membrane group (experimental group). The comparison periods were 7, 15, 30, and 60 days postoperatively. Histological, histometric, and immunohistochemical analyses were performed. The quantitative data were subjected to 2-way ANOVA and Tukey's post-test, and p < 0.05 was considered significant. At 30 and 60 days postoperatively, the BG group showed more healing of the surgical wound than the other groups, with a high amount of newly formed bone (p < 0.001), while the BC group showed mature connective tissue filling the defect. The inflammatory cell count at postoperative days 7 and 15 was higher in the BC group than in the BG group (Tukey's test, p = 0.006). At postoperative days 30 and 60, the area of new bone formed was greater in the BG group than in the other groups (p < 0.001). Immunohistochemical analysis showed moderate and intense immunolabeling of osteocalcin and osteopontin at postoperative day 60 in the BG and BC groups. Thus, despite the promising application of the BC membrane in soft-tissue repair, it did not induce bone repair in rat calvaria.
ABSTRACT
Central cemento-ossifying fibroma is characterized by the combined production of osteoid and cementoid tissue. Radiographically, this lesion is presented as an outlined cortical and variable radiopaque spots, also can be present complete radiolucent or different degrees of radiopacity. The recommended treatment is curettage or enucleation, and the recurrence rate is less than 5%. Considering that surgical treatment is invasive, mainly in large lesions, this study aims to report a patient in whom conservative treatment was carried out by involving the preservation of teeth, with a long-term follow-up. A 48-year-old black female patient, diagnosed with central cemento-ossifying fibroma in mandible, treated conservatively and a 2 years of follow-up. It was concluded that the conservative treatment with a long term of follow-up for maintaining teeth was satisfactory.
Subject(s)
Conservative Treatment , Fibroma, Ossifying/therapy , Mandibular Neoplasms/therapy , Female , Fibroma, Ossifying/diagnostic imaging , Humans , Mandibular Neoplasms/diagnostic imaging , Middle AgedABSTRACT
Este estudo objetivou avaliar a eficácia da membrana de celulose bacteriana (Nanoskin® ) no reparo ósseo de defeitos críticos de 8mm em calota de ratos machos adultos quando comparada à membrana colágena Bio-Gide® . Foram utilizados 72 ratos machos adultos divididos em três grupos experimentais: grupo coágulo (GC), grupo Bio-Gide® (BG) e grupo chá verde (CV). Os períodos de estudo foram de 7, 14 30 e 60 dias após a criação do defeito cirúrgico e aposição da membrana. Foram realizadas a análise histológica, histométrica e imunoistoquímica. Foi possível observar que aos 30 e 60 dias de reparo, o grupo BG apresentou melhor preenchimento do defeito cirúrgico, com maior quantidade de tecido ósseo. Já o grupo CV apresentou preenchimento do defeito com grande quantidade de tecido conjuntivo maduro. Os dados obtidos na análise histométrica revelam que aos 30 e 60 dias a área de osso neoformado teve grande discrepância para o grupo BG em relação ao CV e GC (Teste de Tukey - P<0,001). Por outro lado, os resultados referentes à análise por imunistoquímica evidenciam marcações moderada e intensa para as proteínas osteocalcina (OC) e osteopontina (OP) tanto para o grupo BG quanto para o CV aos 60 dias de reparo. Pode-se concluir que apesar de a membrana de chá verde apresentar aspectos promissores no reparo de tecido mole, neste estudo não permitiu o reparo ósseo em calotas de ratos, quando comparada à Bio-Gide®(AU)
The present study aimed to evaluate the efficiency of bacterial cellulose membrane (Nanoskin® ) in the bone repair of 8 mm critical-size defects in male adult rat calvaria compared to collagen membrane BioGide® . It was used 72 adult male rats divided into three experimental groups: Clot group (CG), Bio-Gide® group (BG) and bacterial cellulose group (BC). The study period were seven, 15, 30 and 60 days after the surgical defect and position of the membrane. It were performed the histologic, histometric and imunohistochemistry analysis and possible to observe that at 30 and 60 days, BG group showed higher filling of the surgical wound with higher amount of new formed bone. The BC group showed a mature connective tissue filling the defect. Data from histometric analysis showed that at 30 and 60 days the new bone formed area was discrepant for BG group compared with BC and CG group (Tukey test P<0,001). On the other hands, immunohistochemistry analysis showed a moderate and intense immunolabeling of osteocalcin and osteopontin at 60 days of bone repair for BG and BC group. It was possible to conclude that despite the BC membrane showing promising aspects in the soft tissue repair, did not allow bone repair in rats calvaria, compared to BioGide®(AU)
