ABSTRACT
OBJECTIVE: The purpose of this study was to analyze the influence of two different irradiation times with 85 mW/cm(2) 830 nm laser on the behavior of mouse odontoblast-like cells. BACKGROUND DATA: The use of low-level laser therapy (LLLT) to stimulate pulp tissue is a reality, but few reports relate odontoblastic responses to irradiation in in vitro models. METHODS: Odontoblast-like cells (MDPC-23) were cultivated and divided into three groups: control/nonirradiated (group 1); or irradiated with 85 mW/cm(2), 830 nm laser for 10 sec (0.8 J/cm(2)) (group 2); or for 50 sec (4.2 J/cm(2)) (group 3) with a wavelength of 830 nm. After 3, 7, and 10 days, it was analyzed: growth curve and cell viability, total protein content, alkaline phosphatase (ALP) activity, calcified nodules detection and quantification, collagen immunolocalization, vascular endothelial growth factor (VEGF) expression, and real-time polymerase chain reaction (PCR) for DMP1 gene. Data were analyzed by Kruskall-Wallis test (α=0.05). RESULTS: Cell growth was smaller in group 2 (p<0.01), whereas viability was similar in all groups and at all periods. Total protein content and ALP activity increased on the 10th day with 0.8 J/cm(2) (p<0.01), as well as the detection and quantification of mineralization nodules (p<0.05), collagen, and VEGF expression (p<0.01). The expression of DMP1 increased in all groups (p<0.05) compared with control at 3 days, except for 0.8 J/cm(2) at 3 days and control at 10 days. CONCLUSIONS: LLLT influenced the behavior of odontoblast-like cells; the shorter time/smallest energy density promoted the expression of odontoblastic phenotype in a more significant way.
Subject(s)
Cell Proliferation/radiation effects , Cell Survival/radiation effects , Low-Level Light Therapy/methods , Odontoblasts/radiation effects , Vascular Endothelial Growth Factor A/radiation effects , Animals , Cells, Cultured/radiation effects , Enzyme-Linked Immunosorbent Assay , In Vitro Techniques , Mice , Odontoblasts/physiology , Radiation Dosage , Random Allocation , Real-Time Polymerase Chain Reaction , Reference Values , Time Factors , Vascular Endothelial Growth Factor A/metabolismABSTRACT
Embora seja doença passível de prevenção, diagnóstico e tratamento adequados e geralmente acessíveis, a sífilis congênita ainda constitui significativo problema de saúde pública. Os autores apresentam um caso de sífilis congênita recente e fazem uma revisão do assunto. Trata-se de uma criança que nasceu com baixo peso, prematura e com manifestações clínicas em vários sistemas e aparelhos (insuficiência respiratória aguda, hepatoesplenomegalia, instabilidade hemodinêmica, icterícia, hipoglicemia, plaquetopenia, leucocitose com desvio para esquerda, hematúria e osteocondrite). Do ponto de vista dermatológico apresentava quadro cutâneo incomum (dermatite esfoliativa com presença de lesões ceratóticas)
