ABSTRACT
The production of cellulase-free endoxylanase by the thermophilic fungus Thermomyces lanuginosus was investigated in semisolid fermentation and liquid fermentation. Different process variables were investigated in semisolid fermentation, employing corncob as the carbon source. The best results were with the following conditions: grain size = 4.5 mm, solid:liquid ratio = 1:2, and inoculum size = 20% (v/v). Corncob, xylan, and xylose were the best inducers for endoxylanase production. Additionally, organic nitrogen sources were necessary for the production of high endoxylanase activities. The crude enzyme had optimum activity at pH 6.0 and 75 degrees C, displaying a high thermostability. The apparent Km and Vmax were 1.77 mg of xylan/mL and 21.5 U/mg of protein, respectively.
Subject(s)
Ascomycota/enzymology , Xylosidases/biosynthesis , Zea mays , Ascomycota/growth & development , Endo-1,4-beta Xylanases , Enzyme Stability , Fermentation , Hot Temperature , Kinetics , Thermodynamics , Xylans/metabolism , Xylose/metabolism , Xylosidases/chemistry , Xylosidases/metabolismABSTRACT
The present work aims to optimize the complex medium for the production of actinomycin-D by S.parvulus. The quantitative influence of the medium components was investigated in order to maximize the antibiotic synthesis. In the original medium, concentrations of actinomycin-D not higher than 245 ug/ml were achieved. In contrast, with the optimized medium the antibiotic concentration reached a value of approximately 530 ug/ml
