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1.
Int J Tuberc Lung Dis ; 17(6): 782-6, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23676162

ABSTRACT

OBJECTIVE: To analyse the contribution of the Ogawa-Kudoh (O-K) swab culture method to the diagnosis of pulmonary tuberculosis (PTB) in four different regions of Brazil. DESIGN: This study was carried out in two phases. Phase 1 was designed to compare the direct swab culture method (O-K) with the culture concentrated method (N-acetyl-L-cysteine-sodium hydroxide [NALC-NaOH]); for this purpose, 569 sputum samples were cultured by both methods. Phase 2 was carried out to assess the contribution of the O-K method to the diagnosis of PTB in four different regions in Brazil, based on the evaluation of 19,163 sputum samples. RESULTS: In the first phase of the study, O-K culture had a sensitivity of 94.8% and specificity of 99.8% in cases confirmed by NALC-NaOH/Löwenstein-Jensen (LJ) culture. In the second phase of the study, the overall contribution of O-K culture compared to acid-fast bacilli (AFB) examination (AFB-/culture+) to the diagnosis of PTB was 29.8%. CONCLUSION: O-K culture contributes significantly to the diagnosis of smear-negative PTB. Importantly, this method allows the recovery of clinical isolates in areas where use of the standard culture centrifuge is impossible, indicating that the O-K swab culture method should become a standard method for TB diagnosis in these regions.


Subject(s)
Sputum/microbiology , Tuberculosis, Pulmonary/diagnosis , Acetylcysteine/chemistry , Adolescent , Adult , Aged , Aged, 80 and over , Bacteriological Techniques/methods , Brazil/epidemiology , Humans , Middle Aged , Prospective Studies , Retrospective Studies , Sensitivity and Specificity , Sodium Hydroxide/chemistry , Tuberculosis, Pulmonary/epidemiology , Young Adult
2.
Int J Tuberc Lung Dis ; 17(2): 225-8, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23317958

ABSTRACT

OBJECTIVE: To investigate risk factors for delayed sputum culture conversion to negative during anti-tuberculosis treatment, with an emphasis on smoking. DESIGN: Nested case-control study of adults with non-cavitary, culture-confirmed pulmonary tuberculosis (TB) participating in an anti-tuberculosis treatment trial in Brazil. A case of delayed culture conversion was a patient who remained culture-positive after 2 months of treatment. Odds ratios with 95% confidence intervals were calculated. RESULTS: Fifty-three cases and 240 control patients were analyzed. Smokers had three-fold greater odds of remaining culture-positive after 2 months of treatment (P = 0.007) than non-smokers, while smokers and ex-smokers who smoked >20 cigarettes a day had two-fold greater odds of remaining culture-positive after 2 months of treatment (P = 0.045). CONCLUSION: Cigarette smoking adversely affects culture conversion during anti-tuberculosis treatment. Support for smoking cessation should be considered to improve outcomes in TB control programs.


Subject(s)
Mycobacterium tuberculosis/isolation & purification , Smoking Cessation , Smoking/adverse effects , Sputum/microbiology , Tuberculosis/drug therapy , Adolescent , Adult , Antitubercular Agents/therapeutic use , Brazil/epidemiology , Confidence Intervals , Follow-Up Studies , Humans , Middle Aged , Mycobacterium tuberculosis/drug effects , Odds Ratio , Prevalence , Retrospective Studies , Risk Factors , Smoking/epidemiology , Smoking Prevention , Treatment Outcome , Tuberculosis/epidemiology , Tuberculosis/microbiology , Young Adult
3.
Int J Tuberc Lung Dis ; 15(2): 281-3, i, 2011 Feb.
Article in English | MEDLINE | ID: mdl-21219695

ABSTRACT

We assessed the effect of a double concentration of supplemental polymyxin B, amphotericin B, nalidixic acid, trimethoprim and azlocillin (PANTA) added to the Mycobacterial Growth Indicator Tube (MGIT) on contamination and positivity rates in 216 sputum cultures. Contamination rates were respectively 12.9% and 5.5% for samples processed using standard and double PANTA concentrations (P = 0.0001, McNemar's test). Thirty-five per cent of cultures performed using standard PANTA and 36.5% of those performed using two-fold PANTA concentrations were positive for Mycobacterium tuberculosis, compared to 25.9% of cultures inoculated on Ogawa medium. These results suggest that the use of MGIT with 2× PANTA may be useful in reducing culture contamination without reducing the diagnostic yield.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteriological Techniques/instrumentation , Disposable Equipment/microbiology , Equipment Contamination/prevention & control , Mycobacterium tuberculosis/isolation & purification , Sputum/microbiology , Tuberculosis, Pulmonary/diagnosis , Amphotericin B/pharmacology , Azlocillin/pharmacology , Culture Media , Dose-Response Relationship, Drug , Humans , Mycobacterium tuberculosis/growth & development , Nalidixic Acid/pharmacology , Polymyxin B/pharmacology , Predictive Value of Tests , Prospective Studies , Trimethoprim/pharmacology , Tuberculosis, Pulmonary/microbiology
4.
Int J Tuberc Lung Dis ; 14(11): 1395-402, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20937178

ABSTRACT

OBJECTIVE: To investigate spatial patterns of the incidence of pulmonary tuberculosis (TB) and its relationship with socio-economic status in Vitoria, Espirito Santo, Brazil. DESIGN: In a 4-year, retrospective, territory-based surveillance study of all new pulmonary TB cases conducted in Vitoria between 2002 and 2006, spatial patterns of disease incidence were compared using spatial clustering statistics (Anselin's local indicators of spatial association [LISA] and Getis-Ord Gi* statistics), smoothed empirical Bayes estimates and model-predicted incidence rates. Spatial Poisson models were fit to examine the relationship between socio-economic status and TB incidence. RESULTS: A total of 651 TB cases were reported across 78 neighborhoods, with rates ranging from 0 to 129 cases per 100,000 population. Moran's I indicated strong spatial autocorrelation among incidence rates (0.399, P < 0.0001), and four areas of high incidence were identified by LISA and Gi* statistics. Smoothed spatial empirical Bayes estimates demonstrate that two of these areas range from 70 to 90 cases/100,000, while the other two range from 40 to 70 cases/100,000. TB incidence and socio-economic status had a significant curvilinear relationship (P = 0.02). CONCLUSIONS: Data derived from these spatial statistical tools will help TB control programs to allocate TB resources to those populations most at risk of increasing TB rates and to target areas where TB control efforts need to be concentrated.


Subject(s)
Models, Statistical , Tuberculosis, Pulmonary/epidemiology , Bayes Theorem , Brazil/epidemiology , Cluster Analysis , Humans , Incidence , Poisson Distribution , Retrospective Studies , Socioeconomic Factors
5.
Int J Tuberc Lung Dis ; 14(11): 1403-10, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20937179

ABSTRACT

SETTING: Primary health clinics in Vitoria, Espirito Santo, Brazil. OBJECTIVE: To identify risk factors associated with patient and health care delays among patients seeking care at primary health clinics. METHODS: A prospective study among tuberculosis (TB) patients diagnosed in Vitoria between 1 January 2003 and 30 December 2007. A questionnaire ascertained the date of onset and duration of TB symptoms and medical records were reviewed. Between-group distributions of delay were compared and multivariate logistic regression was performed. RESULTS: Of 304 patients, 296 (97%) reported at least one TB symptom presenting for the first time to a qualified health service; 244 (80%) reported cough > 3 weeks. Median health care delay was 30 days (range 5-68), and median total delay was 110 days (range 26-784). Multivariate analysis revealed any cough (OR(adj) 7.35, 95%CI 2.40-22.5) and weight at TB diagnosis < 60 kg (OR(adj) 5.92, 95%CI 1.83-19.1) to be associated with patient delay of ≥ 30 days. Factors increasing risk of prolonged delay (≥ 90 days) were age ≥ 30 years (OR(adj) 1.93, 95%CI 1.09-3.43) and chest pain (OR(adj) 2.42, 95%CI 1.29-4.53). CONCLUSION: Improving health care workers' education regarding TB symptoms and implementing active case finding in targeted populations may reduce delays.


Subject(s)
Cough/diagnosis , Delayed Diagnosis/statistics & numerical data , Tuberculosis, Pulmonary/diagnosis , Adult , Age Factors , Brazil/epidemiology , Chest Pain/diagnosis , Chest Pain/etiology , Cough/etiology , Female , Humans , Logistic Models , Male , Multivariate Analysis , Primary Health Care/statistics & numerical data , Prospective Studies , Risk Factors , Time Factors , Tuberculosis, Pulmonary/epidemiology
6.
Int J Tuberc Lung Dis ; 13(12): 1572-5, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19919781

ABSTRACT

This study compared the effect of using two different concentrations of sodium hydroxide (NaOH) in the N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) method for sputum decontamination on smear and culture positivity and the proportion of contaminated cultures: 14% of cultures were contaminated using the standard final 1% NaOH concentration during processing compared to 11% contaminated cultures using a final 1.25% NaOH concentration (P < 0.008). The proportion of cultures positive for mycobacteria decreased from 21% to 11% for sputum processed with 1% and 1.25% final NaOH concentrations, respectively (P < 0.001). Our findings suggest that a small reduction in culture contamination did not justify the considerable loss of positive cultures.


Subject(s)
Decontamination/methods , Sodium Hydroxide/chemistry , Sputum/microbiology , Acetylcysteine/chemistry , Bacteriological Techniques , Humans , Mycobacterium/isolation & purification , Prospective Studies , Specimen Handling , Tuberculosis/diagnosis , Tuberculosis/microbiology
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