Mutational analysis and genetic cloning of the agnostic locus, which regulates learning ability in Drosophila.

P-insertion mutations were obtained and localized by in situ methods at the agnostic gene (agn: 1-38.9; 11AB) in Drosophila. All agn mutants showed a wide spectrum of pleiotropic effects: an EMS-induced mutation of the agn-ts398 improved the ability to develop a conditioned defensive response and increased the activity of cAMP metabolic enzymes; spontaneous mutation of agnX1 showed morphological defects of the brain. P-insertion mutations were used to clone the gene; a restriction map of 80 kb in length was determined, and the insertion was localized to a fragment of 9 kb.

[Mutational analysis and genetic cloning of the agnostic locus controlling learning ability in Drosophila]. / Mutatsionnyi analiz i geneticheskoe klonirovanie lokusa agnostic, kontroliruiuschego sposobnost'k obucheniiu, u drozofily.

The ts-mutations of the agnostic gene either increased or decreased the activity of the AC and PDE. A chromosomal deficiency in the 11B region failed to complement with the agnostic behavioral defect like agn P29 and P40, led to an increased proportion of the PDE-1 in total PDE and to the structural defects in the central complex thus indicating that the P insertion is responsible for the mutant agnostic phenotype.

[The detection of the human immunodeficiency virus antigen in the lymphoid cells of seropositive subjects]. / Vyiavlenie antigena virusa immunodefitsita cheloveka v limfoidnykh kletkakh seropozitivnykh lits.

Cells from anti-HIV-positive persons were used in experiments for virus isolation. The RT-activity, viral antigen, nucleic acids, electron microscopic morphology, and infectivity were studied. The data presented allow a conclusion that the virus was isolated. These data confirmed the previous diagnosis of HIV infection.