ABSTRACT
The purified calf spleen extract (I fraction CM-cellulose), possessing a highly active natural inhibitor of DNAase I, increases the survival rate of gamma-irradiated animals as opposed to irradiated controls. A possible role of DNAase I inhibitor in the radioprotective effect is discussed.
Subject(s)
Endodeoxyribonucleases/antagonists & inhibitors , Radiation Injuries, Experimental/prevention & control , Radiation-Protective Agents/therapeutic use , Spleen/analysis , Tissue Extracts/therapeutic use , Animals , Cattle , Cobalt Radioisotopes , Cricetinae , Deoxyribonuclease I , Gamma Rays , Male , Mesocricetus , MiceABSTRACT
Fractions of calf spleen extracts possessing the increased potency to inhibit the activity of DNase 1 were obtained by chromatography on columns with Sephadex G-200 and CM-cellulose. The fractions containing the most powerful inhibitor of DNase 1 increased the survival rate of sublethally irradiated mice and hamsters. A possible role of the DNase 1 inhibitor in cure effects is discussed.
Subject(s)
Deoxyribonucleases/antagonists & inhibitors , Radiation Injuries, Experimental/prevention & control , Radiation-Protective Agents , Spleen/enzymology , Animals , Cattle , Chemical Fractionation , Cricetinae , Deoxyribonucleases/therapeutic use , Male , Mice , Spleen/analysis , Tissue Extracts/therapeutic useABSTRACT
An increase in the DNase I activity was revealed in the serum of mice after intraperitoneal injection of different antigens. This reaction is related to the antigen nature and is dissimilar in mice of different strains. The increased activity of the inhibitor in the spleen of DBA/2 and BALB/c mice was discovered after intravenous injection of bovine DNase I. A potential role of serum DNase I in the system DNase I-inhibitor is discussed.
Subject(s)
Deoxyribonucleases/blood , Endonucleases/blood , Spleen/enzymology , Animals , Deoxyribonuclease I , Deoxyribonucleases/antagonists & inhibitors , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Inbred DBASubject(s)
Adjuvants, Immunologic , Polymers/pharmacology , Proteins/pharmacology , Pyran Copolymer/pharmacology , Spleen/drug effects , Animals , Antibody Formation/drug effects , DNA/pharmacology , Deoxyribonucleases/antagonists & inhibitors , Drug Evaluation, Preclinical , Hematopoiesis/drug effects , Male , Mice , Mice, Inbred CBA , Radiation Injuries, Experimental/drug therapy , Time FactorsABSTRACT
Activity of the lysosomal hydrolases DNAase II, acid RNAase and acid phosphatase was studied in the mouse liver and spleen under developing Friend's viral leukemia. The activity of DNAase II in the liver was considerably increased from the 12th day after inoculation of virus-containing material and reached the maximum by the 20th day of the experiment. The activity of acid phosphatase was changed insignificantly while that of acid RNAase showed no deviations from the control level. A possible role of the lysosomal hydrolases in leukemia development is discussed.
Subject(s)
Acid Phosphatase/metabolism , Deoxyribonucleases/metabolism , Leukemia, Experimental/enzymology , Lysosomes/enzymology , Ribonucleases/metabolism , Animals , Friend murine leukemia virus , Liver/enzymology , Liver/ultrastructure , Male , Mice , Mice, Inbred DBA , Spleen/enzymologyABSTRACT
Synthetic polyanion pyran (copolimer of divinyl ether and maleic anhydride) injected to mice increases the titre of antibody to sheep red blood cells as well as the activity of serum DNAase I and DNAase I splenic inhibitor. Simultaneously a growth of the spleen weight takes place. A possible role of the DNAase I inhibitor system in the mechanism of the adjuvant action of synthetic polyanion is discussed.
Subject(s)
Actins/metabolism , Deoxyribonucleases/antagonists & inhibitors , Spleen/drug effects , Adjuvants, Immunologic , Animals , Anion Exchange Resins , Antibodies/analysis , Cattle , Enzyme Activation/drug effects , Erythrocytes/immunology , Immunization , Male , Mice , Mice, Inbred CBA , Polymers/pharmacology , Pyrans/pharmacology , Sheep/immunology , Spleen/metabolism , Time FactorsABSTRACT
An increase in the DNAase 1 activity in the serum and an increase in the DNAase inhibitor activity in the spleen in the development of virus Friend leukemia were demonstrated. Increased activity of the inhibitor in the spleen was also revealed after intravenous injection of exogenous DNAase 1 into mice. A potential role of serum DNAase 1 in the development of experimental leukemia is discussed.
Subject(s)
Deoxyribonucleases/blood , Leukemia, Experimental/etiology , Animals , Cattle , Deoxyribonucleases/antagonists & inhibitors , Enzyme Inhibitors/biosynthesis , Friend murine leukemia virus , Male , Mice , Spleen/enzymologyABSTRACT
A developing Friend's viral leukemia is accompanied by a 2,0--2,5-fold increase in the activity of lysosomal DNAse (DNAse II) in mouse liver as compared to normal. The increase in activity is observed on the 10--12th day after inoculation of virus-containing material and reaches its maximum on the 20th post-inoculation day. The increase in DNAse II activity is due to activation of the lysosomal system of Kupffer's and endothelial cells of the liver. The activity of mitochondrial DNAse (DNAse I) in the livers of leukemic mice showed no deviations from the normal level. A possible role of DNAse II in the protective response of the organism is discussed.
Subject(s)
Deoxyribonucleases/metabolism , Isoenzymes/metabolism , Leukemia, Experimental/enzymology , Animals , Endothelium/enzymology , Friend murine leukemia virus , Kupffer Cells/enzymology , Lysosomes/enzymology , Male , Mice , Mice, Inbred BALB C , Mice, Inbred Strains , Mitochondria, Liver/enzymologyABSTRACT
Bacillus subtilis transformation was conducted in the presence of dimethylsulfoxide and polyethyleneglycol. B. subtilis transformation was most frequent under the effect of 0.1% dimethylsulfoxide and was not altered significantly by polyethyleneglycol. As suggested, an increase of the transformation frequency was associated with the change of the membrane permeability under the influence of dimethylsulfoxide of with the altered activity of the membrane DNase.
Subject(s)
Bacillus subtilis/drug effects , Dimethyl Sulfoxide/pharmacology , Transformation, Bacterial/drug effects , Bacillus subtilis/genetics , Polyethylene Glycols/pharmacologyABSTRACT
Immune gamma-globulins containing antibodies to bovine DNAse I inhibit activities of bovine and mouse DNAse I both in vitro and in vivo. Bovine DNAse I was used as exogenous DNAse I in the in vivo studies and was injected to mice intraperitoneally in combination with gamma-globulins. The serous fluid of mice was used as a source of endogenous DNAse I. Both in in vitro and in vivo studies immune gamma-globulins caused a practically complete inhibition of bovine DNAse I activity, whereas the activity of mouse DNAse I (endogenous) was inhibited by 60-80%. Nonimmune gamma-globulins had no inhibitory effects whatsoever.
Subject(s)
Deoxyribonucleases/antagonists & inhibitors , gamma-Globulins , Animals , Antibody Specificity , Cattle , Mice , Species SpecificityABSTRACT
Bacillus subtilis transformation was conducted in the abdominal cavity of mice. The frequency of transformation was considerable decreased when bovine DNA-ase 1 (3-- 5microgram) was injected intraperitoneally to these animals. Immune rabbit gamma-globulins containing antibodies to bovine DNA-ase 1 inhibited in vivo the activity of DNA-ase 1, protected transforming DNA from the hydrolyzing effect of this enzyme. This model can be utilized in search for ways to preserve DNA injected into the animal organism for the purpose of genetical engineering.
